Expressional Comparison of Glucose Cotransporter Isoforms in the Rat Epididymis During Postnatal Development
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Glucose is a major source of metabolic fuel and lipid and protein syntheses. Transport of glucose into the cell is regulated by an action of glucose transport‐associated transporters, especially solute carriers 2A(Slc2a, protein symbol GLUT). The present study was focused on examination of mRNA expression of various Slc2a isoforms in the epididymis during postnatal development. Total RNAs isolated from different epididymal segments(caput, corpus, and caudal epididymis) were utilized for real-time polymerase chain reaction analyses. Results showed that Slc2a 1, 3, 4, 5, and 8 were expressed in the entire epididymal regions. In addition, the abundance of these Slc2a isoforms’ transcripts was different within each epididymal regions. Moreover, the present study showed differential expression of these Slc2a isoforms among different epididymal segments according to postnatal ages. The current study suggests that glucose transport in the epididymis via various Slc2a isoforms would be necessary for maintenance of the epididymal functions.
Abstract
Glucose is a major source of metabolic fuel and lipid and protein syntheses. Transport of glucose into the cell is regulated by an action of glucose transport‐associated transporters, especially solute carriers 2A(Slc2a, protein symbol GLUT). The present study was focused on examination of mRNA expression of various Slc2a isoforms in the epididymis during postnatal development. Total RNAs isolated from different epididymal segments(caput, corpus, and caudal epididymis) were utilized for real-time polymerase chain reaction analyses. Results showed that Slc2a 1, 3, 4, 5, and 8 were expressed in the entire epididymal regions. In addition, the abundance of these Slc2a isoforms’ transcripts was different within each epididymal regions. Moreover, the present study showed differential expression of these Slc2a isoforms among different epididymal segments according to postnatal ages. The current study suggests that glucose transport in the epididymis via various Slc2a isoforms would be necessary for maintenance of the epididymal functions.
참고문헌 (34)
- Abe, H, "Molecular cloning and mRNA expression of the bovine insulin-responsive glucose transporter(GLUT4)", J. Anim. Sci, 75, pp.182~188 (1997)
- Burant, C. F, "Fructose transporter in human spermatozoa and small intestine is GLUT5", J. Biol. Chem, 267, pp.14523~14526 (1992)
- Carosa, E, "Ontogenetic profile and thyroid hormone regulation of type-1 and type-8 glucose transporters in rat Sertoli cells", Int. J. Androl, 28, pp.99~106 (2005)
- Chen, Y, "Expression and regulation of glucose transporter 8 in rat Leydig cells", J Endocrinol, 179, pp.63~72 (2003)
- Cornwall, G. A, "New insights into epididymal biology and function", Hum. Reprod. Update, 15, pp.213~227 (2009)
- Corpe, C. P, "Cloning and functional characterization of the mouse fructose transporter, GLUT5", Biochim. Biophys. Acta, 1576, pp.191~197 (2002)
- Cosentino, M. J, "Structure and function of the epididymis", Urol. Res, 14, pp.229~240 (1986)
- Davidson, N. O, "Human intestinal glucose transporter expression and localization of GLUT5", Am. J. Physiol, 262, pp.795~800 (1992)
- Del Rio, A. G, "High-affinity binding of T3 to epididymis nuclei", Arch. Androl, 44, pp.187~191 (2000)
- Doege, H, "GLUT8, a novel member of the sugar transport facilitator family with glucose transport activity", J. Biol. Chem, 275, pp.16275~16280 (2000)
- Duhlmeier, R, "Mechanisms of insulin-dependent glucose transport into porcine and bovine skeletal muscle", Am. J. Physiol. Regul. Integr. Comp. Physiol, 289, pp.187~197 (2005)
- Galardo, M. N, "Regulation of expression of Sertoli cell glucose transporters 1 and 3 by FSH, IL1 beta, and bFGF at two different time- points in pubertal development", Cell Tissue Res, 334, pp.295~304 (2008)
- Gonzalez, E, "Insulin signaling diverges into Akt-dependent and–independent signals to regulate the recruitment", docking and the fusion of GLUT4 vesicles to the plasma membrane, 2006, pp.17, 4484~4493
- Haber, R. S, "Tissue distribution of the human GLUT3 glucose transporter", Endocrinology, 132, pp.2538~2543 (1993)
- Kajihara, T, "LPS-induced transient testicular dysfunction accompanied by apoptosis of testicular germ cells in mice", Med. Mol. Morphol, 39, pp.203~208 (2006)
- Kujala, M, "Expression of ion transport-associated proteins in human efferent and epididymal ducts", Reproduction, 133, pp.775~784 (2007)
- Lei, Z. M, "Epididymal phenotype in luteinizing hormone receptor knockout animals and its response to testosterone replacement therapy", Biol. Reprod, 68, pp.888~895 (2003)
- Li, Q, "Cloning and functional characterization of the human GLUT7 isoform SLC2A7 from the small intestine", Am. J. Physiol. Gastrointest. Liver Physiol, 287, pp.236~242 (2004)
- Mueckler, M, "Sequence and structure of a human glucose transporter", Science, 229, pp.941~945 (1985)
- Nualart, F, "Glucose transporters in sex steroid hormone related cancer" (2009), Curr. Vasc. Pharmacol
- Pietrement, C, "Distinct expression patterns of different subunit isoforms of the V-ATPase in the rat epididymis", Biol. Reprod, 74, pp.185~194 (2006)
- Rand, E. B, "Sequence, tissue distribution, and functional characterization of the rat fructose transporter GLUT5", Am. J. Physiol, 264, pp.1169~1176 (1993)
- Schürmann, A, "The glucose transport facilitator GLUT8 is predominantly associated with the acrosomal region of mature spermatozoa", Cell. Tissue Res, 307, pp.237~242 (2002)
- Setty, B. S, "Functional maturation of the epididymis in the rat", J. Reprod. Fert, 49, pp.317~322 (1977)
- Shepherd, P. R, "Distribution of GLUT3 glucose transporter protein in human tissues", Biochem. Biophys. Res. Commun, 188, pp.149~154 (1992)
- Stephens, J. M, "Differential regulation of glucose transporter gene expression in adipose tissue or septic rats", Biochem. Biophys. Res. Commun, 183, pp.417~422 (1992)
- Ulisse, S, "Thyroid hormone stimulates glucose transport and GLUT1 mRNA in rat Sertoli cells", Mol. Cell. Endocrinol, 87, pp.131~137 (1992)
- Widdas, W. F, "Old and new concepts of the membrane transport for glucose in cells", Biochem. Biophys. Acta, 947, pp.385~404 (1988)
- Wood, I. S, "Glucose transporters (GLUT and SGLT): expanded families of sugar transport proteins", Br. J. Nutr, 89, pp.3~9 (2003)
- Wright, E.M, "The sodium", glucose cotransport family SLC5, 2004, pp.447, 510~518
- Yamashita, S, "Localization of estrogen and androgen receptor in male reproductive tissues of mice and rats", Anat. Rec. A Discov. Mol. Cell. Evol. Biol, 279, pp.768~778 (2004)
- Zhao, F.-Q, "Distribution of mammalian facultative glucose transporter messenger RNA in bovine tissues", Int. J. Biochem, 25, pp.1897~1903 (1993)
- Zhao, F.-Q, "Functional properties and genomics of glucose transporters", Curr. Genomics, 8, pp.113~128 (2007)
- Zhao, F.-Q, "Bovine glucose transporter GLUT8: cloning, expression, and developmental regulation in mammary gland", Biochim, Biophys. Acta, 1680, pp.103~113 (2004)