지채(Triglochin maritimum)입식에 따른 바이오플락 사육수 내 세균 군집의 변화
Changes in the Biofloc Bacterial Community Caused by Planting of Triglochin maritimum
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
In this study, changes in biofloc-related and pathogenic bacteria in both low and high concentration biofloc breeding water planted with the halophyte (Triglochin maritimum were investigated). In the low-concentration biofloc breeding water, the ratio of bacteria related to the nitrogen cycle was initially 59.57% and, it decreased to 26.57% at the end of the experiment while other bacteria (excluding nitrogen-cycling bacteria and vibrios) increased from 38.75% to 73.43%. However, the planted experimental group maintained a relatively high ratio of nitrogen cycling bacteria at 58%. In the high-concentration experimental group, bacteria related to the initial nitrogen cycle, non-pathogenic vibrios, and pathogenic vibrios were 11.60, 36.28, and 20.14%, respectively. Finally, nitrogen-cycling bacteria were 36.47% in the control group and 37.55% in the planted group. The total number of vibrios decreased by 46.54% in the planted group and 48.01% in the control group, indicating a significant decrease in both experimental groups. However, the residual rate of pathogenic vibrios was 4.48% in the control group and 0.54% in the planted group. Overall, the planted group showed decreasing harmful bacteria and increasing useful bacteria.
Abstract
In this study, changes in biofloc-related and pathogenic bacteria in both low and high concentration biofloc breeding water planted with the halophyte (Triglochin maritimum were investigated). In the low-concentration biofloc breeding water, the ratio of bacteria related to the nitrogen cycle was initially 59.57% and, it decreased to 26.57% at the end of the experiment while other bacteria (excluding nitrogen-cycling bacteria and vibrios) increased from 38.75% to 73.43%. However, the planted experimental group maintained a relatively high ratio of nitrogen cycling bacteria at 58%. In the high-concentration experimental group, bacteria related to the initial nitrogen cycle, non-pathogenic vibrios, and pathogenic vibrios were 11.60, 36.28, and 20.14%, respectively. Finally, nitrogen-cycling bacteria were 36.47% in the control group and 37.55% in the planted group. The total number of vibrios decreased by 46.54% in the planted group and 48.01% in the control group, indicating a significant decrease in both experimental groups. However, the residual rate of pathogenic vibrios was 4.48% in the control group and 0.54% in the planted group. Overall, the planted group showed decreasing harmful bacteria and increasing useful bacteria.
참고문헌 (59)
- Godwin Abakari, "Dynamics of nitrogenous compounds and their control in biofloc technology (BFT) systems: A review", Aquaculture and Fisheries, 6, pp.441~447 (2020), Elsevier BV[DOI]
- Akram R. Alaboudi, "Detection, identification, and prevalence of pathogenic Vibrio parahaemolyticus in fish and coastal environment in Jordan", Journal of Food Science, 81(1), pp.M130~M134 (2015), Wiley[DOI]
- Carmen Amaro, "The fish pathogen Vibrio vulnificus biotype 2: Epidemiology, phylogeny, and virulence factors involved in warm-water vibriosis", Microbiology Spectrum, 3(3), pp.1~23 (2015), American Society for Microbiology[DOI]
- LR Aoki, "Restoration enhances denitrification and DNRA in subsurface sediments of Zostera marina seagrass meadows", Marine Ecology Progress Series, 602, pp.87~102 (2018), Inter-Research Science Center[DOI]
- Viswanathan Baskaran, "Microbial community profiling of ammonia and nitrite oxidizing bacterial enrichments from brackishwater ecosystems for mitigating nitrogen species", Scientific Reports, 10(1), pp.5201~ (2020), Springer Science and Business Media LLC[DOI]
- Christian Boestfleisch, "Changes in secondary metabolites in the halophytic putative crop species Crithmum maritimum L., Triglochin maritima L. and Halimione portulacoides (L.) Aellen as reaction to mild salinity", PLOS ONE, 12(4), pp.e0176303~ (2017), Public Library of Science (PLoS)[DOI]
- Luis Otavio Brito, "Water quality, Vibrio density and growth of Pacific white shrimp Litopenaeus vannamei (Boone) in an integrated biofloc system with red seaweed Gracilaria birdiae (Greville)", Aquaculture Research, 47(3), pp.940~950 (2014), Hindawi Limited[DOI]
- Shuanghu Cai, "AcfA is an essential regulator for pathogenesis of fish pathogen Vibrio alginolyticus", Veterinary Microbiology, 213, pp.35~41 (2018), Elsevier BV[DOI]
- A. Carraturo, "Inhibition of Vibrio parahaemolyticus by a bacteriocin-like inhibitory substance (BLIS) produced by Vibrio mediterranei 1", Journal of Applied Microbiology, 101(1), pp.234~241 (2006), Oxford University Press (OUP)[DOI]
- Carreira C, "Genomic organization, gene expression and activity profile of Marinobacter hydrocarbonoclasticus denitrification enzymes", PeerJ, 6, pp.e5603~ (2018)[DOI]
- 조재황, "대왕범바리 (Epinephelus fuscoguttatus ♀×E. lanceolatus ♂)의 아질산 급성노출에 따른 내성한계: 혈액성상 및 혈장성분의 변화", 환경생물, 38(1), pp.93~100 (2020), 한국환경생물학회[DOI]
- 조영록, "사육수 비교환방식을 이용한 포장 사육지에서의 흰다리새우, Litopenaeus vannamei (Boone, 1931)의 초고밀도 양식", 한국수산과학회지, 43(4), pp.331~339 (2010), 한국수산과학회
- Byron C. Crump, "Metatranscriptomics and Amplicon Sequencing Reveal Mutualisms in Seagrass Microbiomes", Frontiers in Microbiology, 9, pp.388~ (2018), Frontiers Media SA[DOI]
- Allyne Elins Moreira da Silva, "Effect of Brachionus plicatilis and Navicula sp. on Pacific white shrimp growth performance, Vibrio, immunological responses and resistance to white spot virus (WSSV) in nursery biofloc system", Aquaculture, 535, pp.736335~ (2021), Elsevier BV[DOI]
- Hettipala Arachchige Darshanee Ruwandeepika, "Pathogenesis, virulence factors and virulence regulation of vibrios belonging to the Harveyi clade", Reviews in Aquaculture, 4(2), pp.59~74 (2012), Wiley[DOI]
- Yale Deng, "Effect of stock density on the microbial community in biofloc water and Pacific white shrimp (Litopenaeus vannamei) gut microbiota", Applied Microbiology and Biotechnology, 103(10), pp.4241~4252 (2019), Springer Science and Business Media LLC[DOI]
- Le Dou, "Sulfide Removal Characteristics, Pathways and Potential Application of a Novel Chemolithotrophic Sulfide-Oxidizing Strain, Marinobacter Sp. Sdsws8", SSRN Electronic Journal, 212, pp.113176~ (2021), Elsevier BV[DOI]
- Jinming Duan, "Characterization of a halophilic heterotrophic nitrification–aerobic denitrification bacterium and its application on treatment of saline wastewater", Bioresource Technology, 179, pp.421~428 (2015), Elsevier BV[DOI]
- James M. Ebeling, "Engineering analysis of the stoichiometry of photoautotrophic, autotrophic, and heterotrophic removal of ammonia–nitrogen in aquaculture systems", Aquaculture, 257(1-4), pp.346~358 (2006), Elsevier BV[DOI]
- Hargreaves JA, "Biofloc Production Systems for Aquaculture", pp.1~12 (2013), Southern Regional Aquaculture Center
- Huang HH, "Bacterial composition and inferring function profiles in the biofloc system rearing Litopenaeus vannamei postlarvae at a low salinity", 2022, pp.474705~ (2022)[DOI]
- Hai-Hong Huang, "Water quality, shrimp growth performance and bacterial community in a reusing-water biofloc system for nursery of Penaeus vananmei rearing under a low salinity condition", Aquaculture Reports, 21, pp.100894~ (2021), Elsevier BV[DOI]
- Jeong JW, "Effect of halophyte extracts on skin moisturization and barrier function", M.S, pp.2020
- Kathia CM, "Probiotics used in biofloc system for fish and crustacean culture : A review", Int J Fish Aquat Sci, 5, pp.120~125 (2017)
- Yun Hee Kang, "A comparison of the bioremediation potential of five seaweed species in an integrated fish‐seaweed aquaculture system: implication for a multi‐species seaweed culture", Reviews in Aquaculture, 13(1), pp.353~364 (2020), Wiley[DOI]
- 김석렬, "바이오플락과 아쿠아포닉스를 이용한 도심형 양식시스템에서의 메기양식", 환경생물, 37(4), pp.545~553 (2019), 한국환경생물학회[DOI]
- 김수경, "흰다리새우 유생의 저염분 순치방법에 따른 생화학적 특성변화", 환경생물, 35(1), pp.6~12 (2017), 한국환경생물학회[DOI]
- Anna Klindworth, "Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies", Nucleic Acids Research, 41(1), pp.e1~ (2012), Oxford University Press (OUP)[DOI]
- Abnormal Climate Report, "KMA(Korea Meteorological Administration)", KMA, 1~190 (2020)
- David D. Kuhn, "Chronic toxicity of nitrate to Pacific white shrimp, Litopenaeus vannamei: Impacts on survival, growth, antennae length, and pathology", Aquaculture, 309(1-4), pp.109~114 (2010), Elsevier BV[DOI]
- Lee YK, "Isolation and structural analysis of bioactive compounds from two west coast halophyte species", M.S, pp.2018
- Li Y, "Rhizobacterial communities of five co-occurring desert halophytes", PeerJ, 6, pp.e5508~ (2018)[DOI]
- Guozhi Luo, "Nitrate accumulation in biofloc aquaculture systems", Aquaculture, 520, pp.734675~ (2020), Elsevier BV[DOI]
- Belinda C. Martin, "Low Light Availability Alters Root Exudation and Reduces Putative Beneficial Microorganisms in Seagrass Roots", Frontiers in Microbiology, 8, pp.2667~ (2018), Frontiers Media SA[DOI]
- W.T. Mook, "Removal of total ammonia nitrogen (TAN), nitrate and total organic carbon (TOC) from aquaculture wastewater using electrochemical technology: A review", Desalination, 285, pp.1~13 (2012), Elsevier BV[DOI]
- Nicholaus R, "The relationship between benthic nutrient fluxes and bacterial community in Aquaculture tail-water treatment systems", 2021, pp.456883~ (2021)[DOI]
- NIFS(National Institute of Fisheries Science), "Standard Manual of Olive Flounder Culture", NIFS, ~ (2016)
- NIFS(National Institute of Fisheries Science), "Technical Manual for the Mullet Culture", NIFS, ~ (2019)
- NIFS(National Institute of Fisheries Science), "Technical Manual for the Sea Bream Aquaculture", NIFS, ~ (2020)
- 박정준, "바이오플락 환경에서 넙치(Paralichthys olivaceus)의에드워드병에 대한 항생제 치료 효과", 한국어병학회지, 34(2), pp.225~231 (2021), 한국어병학회
- A. Panigrahi, "Bio-augmentation of heterotrophic bacteria in biofloc system improves growth, survival, and immunity of Indian white shrimp Penaeus indicus", Fish Shellfish Immunol, 98, pp.477~487 (2020), Elsevier BV[DOI]
- Si Pang, "Characterization of bacterial community in biofilm and sediments of wetlands dominated by aquatic macrophytes", Ecological Engineering, 97, pp.242~250 (2016), Elsevier BV[DOI]
- Andrew J. Ray, "Water quality dynamics and shrimp (Litopenaeus vannamei) production in intensive, mesohaline culture systems with two levels of biofloc management", Aquacultural Engineering, 45(3), pp.127~136 (2011), Elsevier BV[DOI]
- Andrew J. Ray, "Comparing a chemoautotrophic-based biofloc system and three heterotrophic-based systems receiving different carbohydrate sources", Aquacultural Engineering, 63, pp.54~61 (2014), Elsevier BV[DOI]
- Jilong Ren, "Characterization of a novel marine aerobic denitrifier Vibrio spp. AD2 for efficient nitrate reduction without nitrite accumulation", Environmental Science and Pollution Research, 28(24), pp.30807~30820 (2021), Springer Science and Business Media LLC[DOI]
- Rodrigo Schveitzer, "Effect of different biofloc levels on microbial activity, water quality and performance of Litopenaeus vannamei in a tank system operated with no water exchange", Aquacultural Engineering, 56, pp.59~70 (2013), Elsevier BV[DOI]
- Sook Kyung Shin, "Effects of stocking density on the productivity and nutrient removal of Agarophyton vermiculophyllum in Paralichthys olivaceus biofloc effluent", Journal of Applied Phycology, 32(4), pp.2605~2614 (2020), Springer Science and Business Media LLC[DOI]
- W.Y Shieh, "Denitrification in the rhizosphere of the two seagrasses Thalassia hemprichii (Ehrenb.) Aschers and Halodule uninervis (Forsk.) Aschers", Journal of Experimental Marine Biology and Ecology, 218(2), pp.229~241 (1997), Elsevier BV[DOI]
- Mona Tabarrok, "Comparison between recirculating aquaculture and biofloc systems for rearing juvenile common carp (Cyprinus carpio): Growth performance, haemato-immunological indices, water quality and microbial communities", Aquaculture Research, 51(12), pp.4881~4892 (2020), Hindawi Limited[DOI]
- Flavia Tarquinio, "The seagrass holobiont: understanding seagrass-bacteria interactions and their role in seagrass ecosystem functioning", FEMS Microbiology Letters, 366(6), pp.fnz057~ (2019), Oxford University Press (OUP)[DOI]
- Gladys Valencia-Castañeda, "Physiological changes in the hemolymph of juvenile shrimp Litopenaeus vannamei to sublethal nitrite and nitrate stress in low-salinity waters", Environmental Toxicology and Pharmacology, 80, pp.103472~ (2020), Elsevier BV[DOI]
- Gladys Valencia-Castañeda, "Acute Toxicity of Ammonia, Nitrite and Nitrate to Shrimp Litopenaeus vannamei Postlarvae in Low-Salinity Water", Bulletin of Environmental Contamination and Toxicology, 101(2), pp.229~234 (2018), Springer Science and Business Media LLC[DOI]
- S. Velmurugan, "Screening and characterization of antimicrobial secondary metabolites from Halomonas salifodinae MPM-TC and its in vivo antiviral influence on Indian white shrimp Fenneropenaeus indicus against WSSV challenge", Journal of King Saud University - Science, 25(3), pp.181~190 (2013), Elsevier BV[DOI]
- Ardhra Vijayan, "Nitrospira as versatile nitrifiers: Taxonomy, ecophysiology, genome characteristics, growth, and metabolic diversity", Journal of Basic Microbiology, 61(2), pp.88~109 (2021), Wiley[DOI]
- David F. Woods, "Microbiome and Functional Analysis of a Traditional Food Process: Isolation of a Novel Species (Vibrio hibernica) With Industrial Potential", Frontiers in Microbiology, 11, pp.647~ (2020), Frontiers Media SA[DOI]
- Zhilin Yuan, "Specialized Microbiome of a Halophyte and its Role in Helping Non-Host Plants to Withstand Salinity", Scientific Reports, 6(1), pp.32467~ (2016), Springer Science and Business Media LLC[DOI]
- Jiachen Yu, "Chronic nitrate exposure cause alteration of blood physiological parameters, redox status and apoptosis of juvenile turbot (Scophthalmus maximus)", Environmental Pollution, 283, pp.117103~ (2021), Elsevier BV[DOI]
- Ling Zhang, "Effects of dietary administration of probiotic Halomonas sp. B12 on the intestinal microflora, immunological parameters, and midgut histological structure of shrimp, Fenneropenaeus chinensis", Journal of the World Aquaculture Society, 40(1), pp.58~66 (2009), Wiley[DOI]
- Zhigang Zhao, "Effect of feed C", N ratio promoted bioflocs on water quality and production performance of bottom and filter feeder carp in minimum-water exchanged pond polyculture system, 2014, pp.434, 442~448[DOI]
