Performance of Microflex LT Biotyper and VITEK MS for Routine Identification of Yeasts
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Dear Editor, The epidemiology of yeast infections is rapidly evolving, leading to the emergence of uncommon yeasts [1]. Rapid identification, followed by appropriate antimicrobial therapy, is associated with lower mortality [2]. Conventional phenotypic methods cannot differentiate certain yeast species accurately [3]. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been introduced in clinical microbiology to facilitate rapid yeast identification [3]. MALDI-TOF MS for yeast identification requires special preparation, similar to that for Mycobacterium species and gram-positive bacteria [4]. We compared the yeast identification capabilities of two MALDI-TOF systems—the Microflex LT Biotyper (Bruker Daltonics, Leipzig, Germany) and the VITEK MS (bioMerieux, Marcy-l’Étoile, France)—with respect to different sample preparation methods.
Abstract
Dear Editor, The epidemiology of yeast infections is rapidly evolving, leading to the emergence of uncommon yeasts [1]. Rapid identification, followed by appropriate antimicrobial therapy, is associated with lower mortality [2]. Conventional phenotypic methods cannot differentiate certain yeast species accurately [3]. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been introduced in clinical microbiology to facilitate rapid yeast identification [3]. MALDI-TOF MS for yeast identification requires special preparation, similar to that for Mycobacterium species and gram-positive bacteria [4]. We compared the yeast identification capabilities of two MALDI-TOF systems—the Microflex LT Biotyper (Bruker Daltonics, Leipzig, Germany) and the VITEK MS (bioMerieux, Marcy-l’Étoile, France)—with respect to different sample preparation methods.
참고문헌 (10)
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