Identification of Candida auris and Closely Related Species Using a New Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry, the ASTA MicroIDSys System
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Candida auris is a multidrug-resistant fungal pathogen emerging worldwide that is closely related to the C. haemulonii species complex. The ASTA MicroIDSys (ASTA, Korea) is a new matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) system developed for species-level identification of microorganisms. However, prior to the current study, the reference database of ASTA MicroIDSys did not include C. auris. We expanded the database by adding 20 reference strains of C. auris and three closely related species belonging to C. haemulonii species complex. Further, we compared the performance of the ASTA system using an expanded database (coreDB v1.27.02) to that of the Biotyper system (Bruker Daltonics, USA) using 91 well-characterized Candida isolates from a Korean collection. In addition, we evaluated the ability of the ASTA system to differentiate between clade II and non-clade II isolates of C. auris. The results revealed that both ASTA and Biotyper systems accurately identified all 73 C. auris isolates. Of the 18 isolates of closely related species (nine C. pseudohaemulonii, seven C. haemulonii, and two C. haemulonii var. vulnera), the ASTA and Biotyper systems correctly identified 16 and 14 isolates, respectively, to the species level. Neither system misidentified any of the 91 isolates. Cluster analyses of the ASTA spectra distinctly discriminated clade II Korean C. auris isolates from the non-clade II isolates obtained from other countries. Our results show that the ASTA system with an expanded database is a reliable platform for the identification of C. auris and closely related species.
Abstract
Candida auris is a multidrug-resistant fungal pathogen emerging worldwide that is closely related to the C. haemulonii species complex. The ASTA MicroIDSys (ASTA, Korea) is a new matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) system developed for species-level identification of microorganisms. However, prior to the current study, the reference database of ASTA MicroIDSys did not include C. auris. We expanded the database by adding 20 reference strains of C. auris and three closely related species belonging to C. haemulonii species complex. Further, we compared the performance of the ASTA system using an expanded database (coreDB v1.27.02) to that of the Biotyper system (Bruker Daltonics, USA) using 91 well-characterized Candida isolates from a Korean collection. In addition, we evaluated the ability of the ASTA system to differentiate between clade II and non-clade II isolates of C. auris. The results revealed that both ASTA and Biotyper systems accurately identified all 73 C. auris isolates. Of the 18 isolates of closely related species (nine C. pseudohaemulonii, seven C. haemulonii, and two C. haemulonii var. vulnera), the ASTA and Biotyper systems correctly identified 16 and 14 isolates, respectively, to the species level. Neither system misidentified any of the 91 isolates. Cluster analyses of the ASTA spectra distinctly discriminated clade II Korean C. auris isolates from the non-clade II isolates obtained from other countries. Our results show that the ASTA system with an expanded database is a reliable platform for the identification of C. auris and closely related species.
참고문헌 (20)
- 이양순, "Comparison of a New Matrix-Assisted Laser Desorption", Ionization Time-of-Flight Mass Spectrometry Platform, ASTA MicroIDSys, With Bruker Biotyper for Species Identification, 2017, pp.37(6), 531~535[DOI]
- Lee H, "Evaluation of a new matrix-assisted laser desorption", ionization time-of-fiight mass spectrometry system for the identiflcation of yeast isolation, 2019, pp.33, e22685~
- Kim D, "Performance evaluation of a new matrix-assisted laser desorption", ionization time-of-fiight mass spectrometry, ASTA MicroIDSys system, in bacterial identiflcation against clinical isolates of anaerobic bacteria, 2020, pp.61, 102131~
- Jung J, "Comparison of the ASTA MicroIDSys and VITEK MS matrix-assisted laser desorption", ionization time-of-fiight mass spectrometry systems for identiflcation of clinical bacteria and yeasts, 2020, pp.26, 1328~1333
- Satoh K, "Candida auris sp. nov., a novel ascomycetous yeast isolated from the external ear canal of an inpatient in a Japanese hospital", Microbiol Immunol, 53, pp.41~44 (2009)
- Kim MN, "Candida haemulonii and closely related species at 5 university hospitals in Korea : identiflcation, antifungal susceptibility, and clinical features", Clin Infect Dis, 48, pp.e57~e61 (2009)
- Lee WG, "First three reported cases of nosocomial fungemia caused by Candida auris", J Clin Microbiol, 49, pp.3139~3142 (2011)
- Abastabar M, "Candida auris otomycosis in Iran and review of recent literature", Mycoses, 62, pp.101~105 (2019)
- Gade L, "Understanding the emergence of multidrug-resistant Candida : using whole-genome sequencing to describe the population structure of Candida haemulonii species complex", Front Genet, 11, pp.554~ (2020)
- Chow NA, "Potential flfth clade of Candida auris, Iran, 2018", Emerg Infect Dis, 25, pp.1780~1781 (2019)
- Welsh RM, "Insights into the unique nature of the East Asian clade of the emerging pathogenic yeast Candida auris", J Clin Microbiol, 57, pp.e00007-19~ (2019)
- Kwon YJ, "Candida auris clinical isolates from South Korea : identiflcation, antifungal susceptibility, and genotyping", J Clin Microbiol, 57, pp.e01624-18~ (2019)
- Lima SL, "Increasing prevalence of multidrug-resistant Candida haemulonii species complex among all yeast cultures collected by a reference laboratory over the past 11 years", J Fungi(Basel), 6, pp.110~ (2020)
- 이현승, "Comparison of the Bruker Biotyper and VITEK MS Matrix- Assisted Laser Desorption", Ionization Time-of-Flight Mass Spectrometry Systems Using a Formic Acid Extraction Method to Identify Common and Uncommon Yeast Isolates, 2017, pp.37(3), 223~230[DOI]
- Pence MA, "Comparison and optimization of two MALDI-TOF MS platforms for the identiflcation of medically relevant yeast species", Eur J Clin Microbiol Infect Dis, 33, pp.1703~1712 (2014)
- Cassagne C, "Evaluation of four pretreatment procedures for MALDI-TOF MS yeast identiflcation in the routine clinical laboratory", Med Mycol, 51, pp.371~377 (2013)
- Khot PD, "Optimization of matrix-assisted laser desorption ionization–time of fiight mass spectrometry analysis for bacterial identification", J Clin Microbiol, 50, pp.3845~3852 (2012)
- Fraser M, "Rapid identiflcation of 6328 isolates of pathogenic yeasts using MALDI-ToF MS and a simplifled, rapid extraction procedure that is compatible with the Bruker Biotyper platform and database", Med Mycol, 54, pp.80~88 (2016)
- Mizusawa M, "Can multidrug-resistant Candida auris be reliably identifled in clinical microbiology laboratories?", J Clin Microbiol, 55, pp.638~640 (2017)
- Chow NA, "Multiple introductions and subsequent transmission of multidrug-resistant Candida auris in the USA : a molecular epidemiological survey", Lancet Infect Dis, 18, pp.1377~1384 (2018)
