Locally delivered antioxidant gel as an adjunct to nonsurgical therapy improves measures of oxidative stress and periodontal disease
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Purpose: The present study has two aims; firstly, it attempts to verify the presence of oxidative stress by estimating the reactive oxygen species (ROS) levels in periodontal pockets ≥ 5 mm as compared to controls. The second aim is to evaluate the effect of lycopene as a locally delivered antioxidant gel on periodontal health and on the gingival crevicular fluid (GCF) levels of 8-hydroxydeoxyguanosine (8-OHdG), a marker of oxidative injury. Methods: Thirty-one subjects participated in this study. In the pretreatment phase, the ROS levels in pockets ≥ 5 mm were measured by flow cytometry. Three sites in each subject were randomly assigned into each of the following experimental groups:sham group, only scaling and root planing (SRP) was done; placebo group, local delivery of placebo gel after SRP; and lycopene group, local delivery of lycopene gel after SRP. Clinical parameters included recording site-specific measures of GCF 8-OHdG,plaque, gingivitis, probing depth, and clinical attachment level. Results: The gel, when delivered to the sites with oxidative stress, was effective in increasing clinical attachment and in reducing gingival inflammation, probing depth, and 8-OHdG levels as compared to the placebo and sham sites. Conclusions: From this trial conducted over a period of 6 months, it was found that locally delivered lycopene seems to be effective in reducing the measures of oxidative stress and periodontal disease.
Abstract
Purpose: The present study has two aims; firstly, it attempts to verify the presence of oxidative stress by estimating the reactive oxygen species (ROS) levels in periodontal pockets ≥ 5 mm as compared to controls. The second aim is to evaluate the effect of lycopene as a locally delivered antioxidant gel on periodontal health and on the gingival crevicular fluid (GCF) levels of 8-hydroxydeoxyguanosine (8-OHdG), a marker of oxidative injury. Methods: Thirty-one subjects participated in this study. In the pretreatment phase, the ROS levels in pockets ≥ 5 mm were measured by flow cytometry. Three sites in each subject were randomly assigned into each of the following experimental groups:sham group, only scaling and root planing (SRP) was done; placebo group, local delivery of placebo gel after SRP; and lycopene group, local delivery of lycopene gel after SRP. Clinical parameters included recording site-specific measures of GCF 8-OHdG,plaque, gingivitis, probing depth, and clinical attachment level. Results: The gel, when delivered to the sites with oxidative stress, was effective in increasing clinical attachment and in reducing gingival inflammation, probing depth, and 8-OHdG levels as compared to the placebo and sham sites. Conclusions: From this trial conducted over a period of 6 months, it was found that locally delivered lycopene seems to be effective in reducing the measures of oxidative stress and periodontal disease.
참고문헌 (40)
- Kiyoshima T, "Oxidative stress caused by a low concentration of hydrogen peroxide induces senescence-like changes in mouse gingival fibroblasts", Int J Mol Med, 30, pp.1007~1012 (2012)
- Battino M, "Oxidative injury and inflammatory periodontal diseases : the challenge of anti-oxidants to free radicals and reactive oxygen species", Crit Rev Oral Biol Med, 10, pp.458~476 (1999)
- Borges I Jr, "Proinflammatory and oxidative stress markers in patients with periodontal disease", Mediators Inflamm, 2007, pp.45794~ (2007)
- Masi S, "Oxidative stress, chronic inflammation, and telomere length in patients with periodontitis", Free Radic Biol Med, 50, pp.730~735 (2011)
- Marchetti E, "Periodontal disease : the influence of metabolic syndrome", Nutr Metab, 9, pp.88~ (2012)
- Govindaraj P, "Mitochondrial dysfunction and genetic heterogeneity in chronic periodontitis", Mitochondrion, 11, pp.504~512 (2011)
- Sugiyama S, "Gingival vascular functions are altered in type 2 diabetes mellitus model and", or periodontitis model, 2012, pp.51, 108~113
- Esen C, "The effects of chronic periodontitis and rheumatoid arthritis on serum and gingival crevicular fluid total antioxidant", oxidant status and oxidative stress index, 2012, pp.83, 773~779
- Kawaguchi K, "Effects of antioxidant polyphenols on TNF-alpha-related diseases", Curr Top Med Chem, 11, pp.1767~1779 (2011)
- Soory M, "Relevance of nutritional antioxidants in metabolic syndrome, ageing and cancer: potential for therapeutic targeting", Infect Disord Drug Targets, 9, pp.400~414 (2009)
- Bagaitkar J, "Tobacco-induced alterations to Porphyromonas gingivalis-host interactions", Environ Microbiol, 11, pp.1242~1253 (2009)
- Kornman KS, "The host response to the microbial challenge in periodontitis : assembling the players", Periodontol 2000, 14, pp.33~53 (1997)
- Darveau RP, "The microbial challenge in periodontitis", Periodontol 2000, 14, pp.12~32 (1997)
- Giannelli M, "Effect of chlorhexidine digluconate on different cell types : a molecular and ultrastructural investigation", Toxicol In Vitro, 22, pp.308~317 (2008)
- D’Aiuto F, "Oxidative stress, systemic inflammation, and severe periodontitis", J Dent Res, 89, pp.1241~1246 (2010)
- Wood N, "The relationship between tomato intake and congestive heart failure risk in periodontitis subjects", J Clin Periodontol, 31, pp.574~580 (2004)
- Chandra RV, "Efficacy of lycopene in the treatment of gingivitis: a randomised, placebo-controlled clinical trial", Oral Health Prev Dent, 5, pp.327~336 (2007)
- Chandra RV, "Efficacy of lycopene as a locally delivered gel in the treatment of chronic periodontitis : smokers vs nonsmokers", Quintessence Int, 43, pp.401~411 (2012)
- Maruyama T, "Supplementation of green tea catechins in dentifrices suppresses gingival oxidative stress and periodontal inflammation", Arch Oral Biol, 56, pp.48~53 (2011)
- Fuhrman B, "Hypocholesterolemic effect of lycopene and beta-carotene is related to suppression of cholesterol synthesis and augmentation of LDL receptor activity in macrophages", Biochem Biophys Res Commun, 233, pp.658~662 (1997)
- Dalcico R, "Protective Mechanisms of Simvastatin in Experimental Periodontal Disease", J Periodontol (2012)
- Kara A, "Immune modulatory and antioxidant effects of melatonin in experimental periodontitis in rats", Free Radic Biol Med, 55, pp.21~26 (2013)
- Ku SK, "Effects of calcium gluconate on experimental periodontitis and alveolar bone loss in rats", Basic Clin Pharmacol Toxicol, 108, pp.241~250 (2011)
- Di Mascio P, "Lycopene as the most efficient biological carotenoid singlet oxygen quencher", Arch Biochem Biophys, 274, pp.532~538 (1989)
- Canakci CF, "Increased levels of 8-hydroxydeoxyguanosine and malondialdehyde and its relationship with antioxidant enzymes in saliva of periodontitis patients", Eur J Dent, 3, pp.100~106 (2009)
- Konopka T, "Total antioxidant status and 8-hydroxy-2’-deoxyguanosine levels in gingival and peripheral blood of periodontitis patients", Arch Immunol Ther Exp, 55, pp.417~422 (2007)
- Turesky S, "Reduced plaque formation by the chloromethyl analogue of victamine C", J Periodontol, 41, pp.41~43 (1970)
- Lobene RR, "A modified gingival index for use in clinical trials", Clin Prev Dent, 8, pp.3~6 (1986)
- Patel SA, "Spectrophotometric methods for the estimation of cephalexin in tablet dosage forms", Indian J Pharma Sci, 68, pp.278~280 (2006)
- Tuter G, "Interleukin-1beta and thiobarbituric acid reactive substance(TBARS)levels after phase I periodontal therapy in patients with chronic periodontitis", J Periodontol, 72, pp.883~888 (2001)
- Battino M, "In vitro antioxidant activities of mouthrinses and their components", J Clin Periodontol, 29, pp.462~467 (2002)
- Neiva RF, "Effects of specific nutrients on periodontal disease onset, progression and treatment", J Clin Periodontol, 30, pp.579~589 (2003)
- Watts T, "Periodontal treatment and glycemic control in diabetic patients : the problem of a possible Hawthorne effect", J Dent Res, 85, pp.294~ (2006)
- Bansal K, "Development of satranidazole mucoadhesive gel for the treatment of periodontitis", AAPS PharmSciTech, 10, pp.716~723 (2009)
- Halliwell B, "Measuring reactive species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean?", Br J Pharmacol, 142, pp.231~255 (2004)
- Palozza P, "Role of lycopene in the control of ROS-mediated cell growth : implications in cancer prevention", Curr Med Chem, 18, pp.1846~1860 (2011)
- Dede FO, "8-hydroxy-deoxyguanosine levels in gingival crevicular fluid and saliva in patients with chronic periodontitis after initial periodontal treatment", J Periodontol, 84, pp.821~828 (2013)
- Jakubowski W, "2,7-dichlorofluorescin oxidation and reactive oxygen species: what does it measure?", Cell Biol Int, 24, pp.757~760 (2000)
- Wolfe KL, "Structure-activity relationships of flavonoids in the cellular antioxidant activity assay", J Agric Food Chem, 56, pp.8404~8411 (2008)
- Myhre O, "Evaluation of the probes 2′,7′-dichlorofluorescin diacetate, luminol, and lucigenin as indicators of reactive species formation", Biochem Pharmacol, 65, pp.1575~1582 (2003)