Ovarian Tissue-Based Hormone Replacement Therapy Recovers Menopause-Related Signs in Mice
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Purpose: In women, menopause manifests with a variety of symptoms related to sex-hormone deficiency. Supplementing steroidhormones with pharmacological drugs has been widely practiced. However, considering the possible complications associatedwith artificial hormone therapy, studies have been conducted to find an alternative to pharmacological hormone replacementtherapy. Accordingly, this study aimed to evaluate the efficacy of tissue-based hormone replacement therapy (tHRT) for treatingpost-menopausal signs and symptoms. Materials and Methods: CD-1 mice were ovariectomized, and the ovaries were cryopreserved. Following artificial induction ofpost-menopausal osteoporosis, cryopreserved ovaries were subcutaneously autografted, and indexes related to bone health weremonitored for 12 weeks. Bone mineral density (BMD), bone mineral contents (BMC), total bone volume (BV), and body fat masswere measured by dual energy X-ray absorptiometry. Uterine atrophy was assessed histologically, and bone microstructures wereimaged by micro-computed tomography analysis. Results: Regardless of the number of grafted ovaries, the BMC, BMD, and BV values of mice that underwent ovary transplantationwere better than those that did not undergo transplantation. The uteruses in these mice were thicker and heavier after auto-trans plantation. Furthermore, the bone microstructure recovered after tHRT. Conclusion: Recovery of menopause-related bone loss and uterine atrophy was achieved through tHRT. Ovarian tissue cryo preservation and transplantation may be applicable not only in patients wanting to preserve fertility but also in sex hormone-de ficient post-menopausal women.
Abstract
Purpose: In women, menopause manifests with a variety of symptoms related to sex-hormone deficiency. Supplementing steroidhormones with pharmacological drugs has been widely practiced. However, considering the possible complications associatedwith artificial hormone therapy, studies have been conducted to find an alternative to pharmacological hormone replacementtherapy. Accordingly, this study aimed to evaluate the efficacy of tissue-based hormone replacement therapy (tHRT) for treatingpost-menopausal signs and symptoms. Materials and Methods: CD-1 mice were ovariectomized, and the ovaries were cryopreserved. Following artificial induction ofpost-menopausal osteoporosis, cryopreserved ovaries were subcutaneously autografted, and indexes related to bone health weremonitored for 12 weeks. Bone mineral density (BMD), bone mineral contents (BMC), total bone volume (BV), and body fat masswere measured by dual energy X-ray absorptiometry. Uterine atrophy was assessed histologically, and bone microstructures wereimaged by micro-computed tomography analysis. Results: Regardless of the number of grafted ovaries, the BMC, BMD, and BV values of mice that underwent ovary transplantationwere better than those that did not undergo transplantation. The uteruses in these mice were thicker and heavier after auto-trans plantation. Furthermore, the bone microstructure recovered after tHRT. Conclusion: Recovery of menopause-related bone loss and uterine atrophy was achieved through tHRT. Ovarian tissue cryo preservation and transplantation may be applicable not only in patients wanting to preserve fertility but also in sex hormone-de ficient post-menopausal women.
참고문헌 (35)
- Prasasya RD, "The ovary", pp.23~49 (2019), Academic Press
- Blume SW, "Medical costs of osteoporosis in the elderly medicare population", Osteoporos Int, 22, pp.1835~1844 (2011)
- Keen RW, "Burden of osteoporosis and fractures", Curr Osteoporos Rep, 1, pp.66~70 (2003)
- Voican A, "Pharmacology" (2012), IntechOpen
- Sittadjody S, "In vivo transplantation of 3D encapsulated ovarian constructs in rats corrects abnormalities of ovarian failure", Nat Commun, 8, pp.1858~ (2017)
- Donnez J, "Livebirth after orthotopic transplantation of cryopreserved ovarian tissue", Lancet, 364, pp.1405~1410 (2004)
- Donnez J, "Children born after autotransplantation of cryopreserved ovarian tissue. a review of 13 live births", Ann Med, 43, pp.437~450 (2011)
- Imbert R, "Safety and usefulness of cryopreservation of ovarian tissue to preserve fertility : a 12-year retrospective analysis", Hum Reprod, 29, pp.1931~1940 (2014)
- Kim SS, "Assessment of long term endocrine function after transplantation of frozen-thawed human ovarian tissue to the heterotopic site: 10 year longitudinal follow-up study", J Assist Reprod Genet, 29, pp.489~493 (2012)
- Silber S, "Duration of fertility after fresh and frozen ovary transplantation", Fertil Steril, 94, pp.2191~2196 (2010)
- Youm HW, "Optimal vitrification protocol for mouse ovarian tissue cryopreservation : effect of cryoprotective agents and in vitro culture on vitrified-warmed ovarian tissue survival", Hum Reprod, 29, pp.720~730 (2014)
- Silva LHFME, "Criopreserved ovarian tissue transplantation and bone restoration metabolism in castrated rats", Rev Col Bras Cir, 45, pp.e1577~ (2018)
- Lee J, "Effects of three different types of antifreeze proteins on mouse ovarian tissue cryopreservation and transplantation", PLoS One, 10, pp.e0126252~ (2015)
- Lee J, "Comparison of the oocyte quality derived from two-dimensional follicle culture methods and developmental competence of in vitro grown and matured oocytes", Biomed Res Int, 2018, pp.7907092~ (2018)
- Kim S, "Principles and practice of fertility preservation", pp.328~341 (2011), Cambridge University Press
- Anderson RA, "Ovarian tissue transplantation for hormone replacement", Reprod Biomed Online, 37, pp.251~252 (2018)
- Silber S, "Fresh and cryopreserved ovary transplantation and resting follicle recruitment", Reprod Biomed Online, 30, pp.643~650 (2015)
- Andersen ST, "Ovarian stimulation and assisted reproductive technology outcomes in women transplanted with cryopreserved ovarian tissue : a systematic review", Fertil Steril, 112, pp.908~921 (2019)
- Jensen AK, "Outcomes of transplantations of cryopreserved ovarian tissue to 41 women in Denmark", Hum Reprod, 30, pp.2838~2845 (2015)
- Agarwal S, "Hormone replacement therapy : would it be possible to replicate a functional ovary?", Int J Mol Sci, 19, pp.3160~ (2018)
- Kristensen SG, "Cryopreservation of ovarian tissue:opportunities beyond fertility preservation and a positive view into the future", Front Endocrinol (Lausanne), 9, pp.347~ (2018)
- Dolmans MM, "Risk of transferring malignant cells with transplanted frozen-thawed ovarian tissue", Fertil Steril, 99, pp.1514~1522 (2013)
- Zhang X, "Fertility preservation in BRCA mutation carriers-efficacy and safety issues : a review", Reprod Biol Endocrinol, 18, pp.11~ (2020)
- Soares M, "Eliminating malignant cells from cryopreserved ovarian tissue is possible in leukaemia patients", Br J Haematol, 178, pp.231~239 (2017)
- Shikanov A, "A method for ovarian follicle encapsulation and culture in a proteolytically degradable 3dimensional system", J Vis Exp, 49, pp.e2695~ (2011)
- Smith RM, "Fibrin-mediated delivery of an ovarian follicle pool in a mouse model of infertility", Tissue Eng Part A, 20, pp.3021~3030 (2014)
- Guo XX, "Prevention of osteoporosis in mice after ovariectomy via allograft of microencapsulated ovarian cells", Anat Rec(Hoboken), 293, pp.200~207 (2010)
- Liu C, "An ovarian cell microcapsule system simulating follicle structure for providing endogenous female hormones", Int J Pharm, 455, pp.312~319 (2013)
- Krotz SP, "In vitro maturation of oocytes via the pre-fabricated self-assembled artificial human ovary", J Assist Reprod Genet, 27, pp.743~750 (2010)
- Phetfong J, "Osteoporosis : the current status of mesenchymal stem cell-based therapy", Cell Mol Biol Lett, 21, pp.12~ (2016)
- Barkholt L, "Risk of tumorigenicity in mesenchymal stromal cell-based therapies--bridging scientific observations and regulatory viewpoints", Cytotherapy, 15, pp.753~759 (2013)
- Yoshida S, "Syngeneic mesenchymal stem cells reduce immune rejection after induced pluripotent stem cell-derived allogeneic cardiomyocyte transplantation", Sci Rep, 10, pp.4593~ (2020)
- Lee J, "A combination of simvastatin and methylprednisolone improves the quality of vitrified-warmed ovarian tissue after auto-transplantation", Hum Reprod, 30, pp.2627~2638 (2015)
- Lee J, "Ovarian injury during cryopreservation and transplantation in mice : a comparative study between cryoinjury and ischemic injury", Hum Reprod, 31, pp.1827~1837 (2016)
- 이정렬, "Effect of Antifreeze Protein on Mouse Ovarian Tissue Cryopreservation and Transplantation", Yonsei Medical Journal, 56(3), pp.778~784 (2015), 연세대학교의과대학[DOI]