A modified hydrostatic microfluidic pumpless device for in vitro murine ovarian tissue culture as research model for fertility preservation
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This study aimed to compare the efficacies of conventional and non-conventional (modified hydrostatic microfluidic pumpless device, MHPD) systems on ovarian tissue culture and in vitro follicle growth using a mouse model. A total of 56 ovarian cortical tissues retrieved from seven wild-type mice were divided into three groups: 1) fresh control, 2) conventional culture system (control), and 3) non-conventional system with MHPD. Ovarian tissues were cultured for 96 hours and evaluated for follicle morphology, developmental stage, intact follicle density, and relative gene expression levels (proliferating cell nuclear antigen, insulin like growth factor 1, BAX, and Bcl-2). Our major data demonstrated that the mean percentage of primary follicle development was increased by the MHPD (P<0.05). In addition, this device could maintain and support follicle development better than the conventional culture systems. However, the overall outcomes were not significantly improved by our first-design prototype. Consequently, nextgeneration platforms should be developed as alternative medical tools for fertility preservation research.
Abstract
This study aimed to compare the efficacies of conventional and non-conventional (modified hydrostatic microfluidic pumpless device, MHPD) systems on ovarian tissue culture and in vitro follicle growth using a mouse model. A total of 56 ovarian cortical tissues retrieved from seven wild-type mice were divided into three groups: 1) fresh control, 2) conventional culture system (control), and 3) non-conventional system with MHPD. Ovarian tissues were cultured for 96 hours and evaluated for follicle morphology, developmental stage, intact follicle density, and relative gene expression levels (proliferating cell nuclear antigen, insulin like growth factor 1, BAX, and Bcl-2). Our major data demonstrated that the mean percentage of primary follicle development was increased by the MHPD (P<0.05). In addition, this device could maintain and support follicle development better than the conventional culture systems. However, the overall outcomes were not significantly improved by our first-design prototype. Consequently, nextgeneration platforms should be developed as alternative medical tools for fertility preservation research.
참고문헌 (15)
- Ataman LM, "Creating a global community of practice for oncofertility", J Glob Oncol, 2, pp.83~96 (2016)
- Anderson RA, "Ovarian tissue cryopreservation for fertility preservation : clinical and research perspectives", Hum Reprod Open, 2017, pp.hox001~ (2017)
- Andreas Obermair, "Fertility-sparing treatment in early endometrial cancer: current state and future strategies", Obstetrics & Gynecology Science, 63(4), pp.417~431 (2020), 대한산부인과학회[DOI]
- Telfer EE, "Future developments : in vitro growth(IVG)of human ovarian follicles", Acta Obstet Gynecol Scand, 98, pp.653~658 (2019)
- Smitz J, "Current achievements and future research directions in ovarian tissue culture, in vitro follicle development and transplantation : implications for fertility preservation", Hum Reprod Update, 16, pp.395~414 (2010)
- Filatov MA, "Female fertility preservation strategies : cryopreservation and ovarian tissue in vitro culture, current state of the art and future perspectives", Zygote, 24, pp.635~653 (2016)
- Salama M, "From bench to bedside : current developments and future possibilities of artificial human ovary to restore fertility", Acta Obstet Gynecol Scand, 98, pp.659~664 (2019)
- Xiao S, "A microfluidic culture model of the human reproductive tract and 28-day menstrual cycle", Nat Commun, 8, pp.14584~ (2017)
- Komeya M, "Pumpless microfluidic system driven by hydrostatic pressure induces and maintains mouse spermatogenesis in vitro", Sci Rep, 7, pp.15459~ (2017)
- Sackmann EK, "The present and future role of microfluidics in biomedical research", Nature, 507, pp.181~189 (2014)
- Aziz AUR, "A microfluidic device for culturing an encapsulated ovarian follicle", Micromachines(Basel), 8, pp.335~ (2017)
- Lo BKM, "In vitro and in vivo mouse follicle development in ovaries and reaggregated ovaries", Reproduction, 157, pp.135~148 (2019)
- Sadeghzadeh Oskouei B, "Evaluation of mouse oocyte in vitro maturation developmental competency in dynamic culture systems by design and construction of a lab on a chip device and its comparison with conventional culture system", Cell J, 18, pp.205~213 (2016)
- Campos Marín A, "Flow perfusion rate modulates cell deposition onto scaffold substrate during cell seeding", Biomech Model Mechanobiol, 17, pp.675~687 (2018)
- Komeya M, "In vitro spermatogenesis in twodimensionally spread mouse testis tissues", Reprod Med Biol, 18, pp.362~369 (2019)