Proteomics Analysis for Identifying Proteins Responding to Low Nitrogen Application in Rice (Oryza sativa L.)
2
조회
0
다운로드
2
인용
0
참고문헌
초록
Abstract
Nitrogen (N) fertilization is essential for alleviating nutrient deficiencies of the world’s population by increasing rice production, one of the most important food crops of our time. Here we established an in vivo hydroponics rice seedling culture system to investigate the physio-biochemical and molecular responses of various rice genotypes to low nitrogen application. Yoshida’s nutrient solution (YS) was used to grow rice seedlings, and at three-week-old the seedlings manifested highly stable and reproducible symptoms, such as reduced shoot growth and length. Out of 12 genetically selected or tested genotypes, almost all (11 genotypes) showed varied degrees of growth reduction response to applied nitrogen (4 and 40 ppm N for treatment and control, respectively), but SR19663-B-B-34-3-3-3-1 showed similar growth as the control though its leaf width was smaller than the control. The leaves of a 11 representative low nitrogen-responsive genotype as BG90-2 were sampled for revealing the protein profiles between low and normal (control) nitrogen application by using two-dimensional gel electrophoresis (2-DGE) followed by staining of separated proteins with silver. Fifty differentially expressed silver stained protein spots were excised from 2-D gels and 41 proteins identified using high-throughput mass spectrometry (MS) using matrix-assisted laser desorption/ionization-time of flight-MS and nano electrospray ionization liquid chromatography tandem MS. These proteins could be assigned as major (energy metabolism, photosynthesis and oxidative stress) and minor functional categories, revealing many novel low N-responsive proteins, including those having energy/photosynthesis, and defense/stress, and iron homeostasis-related functions.
참고문헌 (22)
- Agrawal GK, "Proteome analysis of differentially displayed proteins as a tool for investigating ozone stress in rice seedlings", 2, pp.947~959 (2002)
- Agrawal G.K, "System, trends & perspectives of proteomics in dicot plants. Part I: Technologies in proteome establishment.", 815, pp.109~123 (2005)
- Agrawal G.K, "System, trends & perspectives of proteomics in dicot plants. Part II: Proteomes of the complex developmental stages", 815, pp.125~136 (2005)
- Agrawal G.K, "System, trends & perspectives of proteomics in dicot plants. Part III: Unraveling the proteomes influenced by the environment, and at the levels of function & genetic relationships", 815, pp.137~145 (2005)
- Agrawal G.K, "Rice proteomics: A cornerstone for cereal food crop proteomes", 25, pp.1~53 (2006)
- Broadbent EE, "Measurement of nitrogen utilization efficiency in rice genotypes", pp.786~791 (1987)
- Cui S, "A proteomic analysis of cold stress responses in rice seedlings", pp.3162~5 3172
- "Directorate of Rice Research", pp.290~299
- Goto F, "Iron fortification of rice seed by the soybean ferritin gene", 17, pp.282~286 (1999)
- Hoai NTT, "Accumulation of some nitrogen compounds in response to salt stress and their relationship with salt tolerance in rice (Oryza sativa L.) seedlings", 41, pp.159~164 (2003)
- Kim DW, "A hydroponic rice seedling culture model system for investigating proteome of salt stress in rice leaf", 26, pp.4521~4539 (2005)
- May MJ, "Glutathione homeostasis in plants implications for environmental sensing and plant development", 49, pp.649~667 (1998)
- Molik S, "The Rieske Fe", S Protein of the Cytochrome b6, pp.2001, 276[DOI]
- Oh MK, "Genotypic differences in nitrogen uptake and utilization for grain yield of rice", 37(4), pp.209~213 (2005)
- Park OK, "Proteomic studies in plants", 37, pp.133~138 (2004)
- Rakwal R, "Defense", stress responses elicited in rice seedlings exposed to the gaseous air pollutant sulfur dioxide, Environ, 2003, pp.49, 223~235
- Scandalios JG, "The rise of ROS", 27, pp.483~486 (2002)
- Singh KG, "Innovative nitrogen management strategies for lowland rice systems Fragile lives in fragile ecosystems", pp.786~791 (1995), International Rice Research Institute, Manila, Philippines
- Sugihara K, "Molecular characterization of cDNA encoding oxygen evolving enhancer protein 1 increased by salt treatment in the mangrove Bruguiera gymnorrhiza", 41, pp.1279~1285 (2000)
- In, "New trend in rice crop production enhancement of nutrient use efficiency New Frontiers in Rice Research"
- Wingler A, "Photorespiration: metabolic pathways and their role in stress protection", 1355, pp.1517~1529 (2000)
- Yoshida S, "Laboratory manual for physiological studies of rice", pp.61~65