Increased Tolerance to Furfural by Introduction of Polyhydroxybutyrate Synthetic Genes to Escherichia coli
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Polyhydroxybutyrate (PHB), the most well-known polyhydroxyalkanoate, is a bio-based, biodegradable polymer that has the potential to replace petroleum-based plastics. Lignocellulose hydrolysate, a non-edible resource, is a promising substrate for the sustainable, fermentative production of PHB. However, its application is limited by the generation of inhibitors during the pretreatment processes. In this study, we investigated the feasibility of PHB production in E. coli in the presence of inhibitors found in lignocellulose hydrolysates. Our results show that the introduction of PHB synthetic genes (bktB, phaB, and phaC from Ralstonia eutropha H16) improved cell growth in the presence of the inhibitors such as furfural, 4-hydroxybenzaldehyde, and vanillin, suggesting that PHB synthetic genes confer resistance to these inhibitors. In addition, increased PHB production was observed in the presence of furfural as opposed to the absence of furfural, suggesting that this compound could be used to stimulate PHB production. Our findings indicate that PHB production using lignocellulose hydrolysates in recombinant E. coli could be an innovative strategy for cost-effective PHB production, and PHB could be a good target product from lignocellulose hydrolysates, especially glucose.
Abstract
Polyhydroxybutyrate (PHB), the most well-known polyhydroxyalkanoate, is a bio-based, biodegradable polymer that has the potential to replace petroleum-based plastics. Lignocellulose hydrolysate, a non-edible resource, is a promising substrate for the sustainable, fermentative production of PHB. However, its application is limited by the generation of inhibitors during the pretreatment processes. In this study, we investigated the feasibility of PHB production in E. coli in the presence of inhibitors found in lignocellulose hydrolysates. Our results show that the introduction of PHB synthetic genes (bktB, phaB, and phaC from Ralstonia eutropha H16) improved cell growth in the presence of the inhibitors such as furfural, 4-hydroxybenzaldehyde, and vanillin, suggesting that PHB synthetic genes confer resistance to these inhibitors. In addition, increased PHB production was observed in the presence of furfural as opposed to the absence of furfural, suggesting that this compound could be used to stimulate PHB production. Our findings indicate that PHB production using lignocellulose hydrolysates in recombinant E. coli could be an innovative strategy for cost-effective PHB production, and PHB could be a good target product from lignocellulose hydrolysates, especially glucose.
참고문헌 (48)
- Ragauskas AJ, "The path forward for biofuels and biomaterials", Science, 311, pp.484~489 (2006)
- Lynd LR, "How biotech can transform biofuels", Nat. Biotechnol, 26, pp.169~172 (2008)
- Sims RE, "An overview of second generation biofuel technologies", Bioresour. Technol, 101, pp.1570~1580 (2010)
- Brodin M, "Lignocellulosics as sustainable resources for production of bioplastics-a review", J. Clean Prod, 162, pp.646~664 (2017)
- Sharma HK, "Biological pretreatment of lignocellulosic biomass for biofuels and bioproducts : an overview", Waste and Biomass Valorization, pp.1~17 (2017)
- Carroll A, "Cellulosic biofuels", Annu. Rev. Plant Biol, 60, pp.165~182 (2009)
- Weijde Tvd, "The potential of C4 grasses for cellulosic biofuel production", Front. Plant Sci, 4, pp.107~ (2013)
- Bhatia SK, "Bioconversion of plant biomass hydrolysate into bioplastic(polyhydroxyalkanoates)using Ralstonia eutropha 5119", Bioresour. Technol, 271, pp.306~315 (2019)
- Kumar P, "Methods for pretreatment of lignocellulosic biomass for efficient hydrolysis and biofuel production", Ind. Eng. Chem, 48, pp.3713~3729 (2009)
- Sun Y, "Hydrolysis of lignocellulosic materials for ethanol production : a review", Bioresour. Technol, 83, pp.1~11 (2002)
- Mills TY, "Cellulosic hydrolysate toxicity and tolerance mechanisms in Escherichia coli", Biotechnol. Biofuels, 2, pp.26~ (2009)
- Saha BC, "Hemicellulose bioconversion", J. Ind. Microbiol. Biotechnol, 30, pp.279~291 (2003)
- Gorsich S, "Tolerance to furfural-induced stress is associated with pentose phosphate pathway genes ZWF1, GND1, RPE1, and TKL1 in Saccharomyces cerevisiae", Appl. Microbiol. Biotechnol, 71, pp.339~349 (2006)
- Barciszewski J, "A mechanism for the in vivo formation of N6-furfuryladenine, kinetin, as a secondary oxidative damage product of DNA", FEBS Lett, 414, pp.457~460 (1997)
- Palmqvist E, "Fermentation of lignocellulosic hydrolysates, II: inhibitors and mechanisms of inhibition", Bioresour. Technol, 74, pp.25~33 (2000)
- Zaldivar J, "Effect of alcohol compounds found in hemicellulose hydrolysate on the growth and fermentation of ethanologenic Escherichia coli", Biotechnol. Bioeng, 68, pp.524~530 (2000)
- Song H-S, "Increase in furfural tolerance by combinatorial overexpression of NAD salvage pathway enzymes in engineered isobutanol-producing E, coli", Bioresour. Technol, 245, pp.1430~1435 (2017)
- Seo H-M, "Combinatorial application of two aldehyde oxidoreductases on isobutanol production in the presence of furfural", J. Ind. Microbiol. Biotechnol, 43, pp.37~44 (2016)
- Wang X, "Increased furfural tolerance due to overexpression of NADH-dependent oxidoreductase FucO in Escherichia coli strains engineered for the production of ethanol and lactate", Appl. Environ. Microbiol, 77, pp.5132~5140 (2011)
- Wang X, "Engineering furfural tolerance in Escherichia coli improves the fermentation of lignocellulosic sugars into renewable chemicals", Proc. Natl. Acad. Sci. USA, 110, pp.4021~4026 (2013)
- Madkour MH, "PHA recovery from bio mass", Biomacromolecules, 14, pp.2963~2972 (2013)
- Steinbuchel A, "Bacterial and other biological systems for polyester production", Trends. Biotechnol, 16, pp.419~427 (1998)
- Wen Q, "Effects of phosphorus and nitrogen limitation on PHA production in activated sludge", J. Environ. Sci, 22, pp.1602~1607 (2010)
- Johnson K, "Influence of the C", N ratio on the performance of polyhydroxybutyrate(PHB)producing sequencing batch reactors at short SRTs, 2010, pp.44, 2141~2152
- 안준모, "Effect of C", N ratio on polyhydroxyalkanoates (PHA) accumulation by Cupriavidus necator and its implication on the use of rice straw hydrolysates, 2015, pp.20(3), 246~253[DOI]
- Sudesh K, "Synthesis, structure and properties of polyhydroxyalkanoates : biological polyesters", Prog. Polym. Sci, 25, pp.1503~1555 (2000)
- Du C, "Polyhydroxyalkanoates production from low-cost sustainable raw materials", Curr. Chem. Biol, 6, pp.14~25 (2012)
- Production of Bio-ethylene−Technology Brief, IEA-ETSAP & IRENA, "Broeren M", International Renewable Energy Agency, ~ (2013)
- Cesário MT, "Enhanced bioproduction of poly-3-hydroxybutyrate from wheat straw lignocellulosic hydrolysates", N. Biotechnol, 31, pp.104~113 (2014)
- Yu J, "Microbial utilization and biopolyester synthesis of bagasse hydrolysates", Bioresour. Technol, 99, pp.8042~8048 (2008)
- Silva L, "Poly-3-hydroxybutyrate(P3HB)production by bacteria from xylose, glucose and sugarcane bagasse hydrolysate", J Ind. Microbiol. Biotechnol, 31, pp.245~254 (2004)
- Dietrich D, "Differential sensitivity of polyhydroxyalkanoate producing bacteria to fermentation inhibitors and comparison of polyhydroxybutyrate production from Burkholderia cepacia and Pseudomonas pseudoflava", BMC Res. Notes, 6, pp.219~ (2013)
- Wang W, "Connecting lignin-degradation pathway with pretreatment inhibitor sensitivity of Cupriavidus necator", Front. Microbiol, 5, pp.247~ (2014)
- Dietrich K, "Model study to assess softwood hemicellulose hydrolysates as the carbon source for PHB production in Paraburkholderia sacchari IPT 101", Biomacromolecules, 19, pp.188~200 (2017)
- Yang YH, "Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate)containing a predominant amount of 3-hydroxyvalerate by engineered Escherichia coli expressing propionate-CoA transferase", J. Appl. Microbiol, 113, pp.815~823 (2012)
- Jeon JM, "Application of acetyl-CoA acetyltransferase(AtoAD)in Escherichia coli to increase 3-hydroxyvalerate fraction in poly(3-hydroxybutyrate-co-3-hydroxyvalerate)", Bioproc. Biosyst. Eng, 40, pp.781~789 (2017)
- Mezzina MP, "A phasin with extra t alents : a po lyhydroxyalkanoate granule-associated protein has chaperone activity", Environ. Microbiol, 17, pp.1765~1776 (2015)
- Phylactides M, "Molecular biology series 3, Tools of molecular biology: gene cloning", Br. J. Hosp. Med, 57, pp.49~50 (1997)
- Braunegg G, "A rapid gas chromatographic method for the determination of poly-β-hydroxybutyric acid in microbial biomass", Appl. Microbiol. Biotechnol, 6, pp.29~37 (1978)
- Bhatia SK, "Microbial biodiesel production from oil palm biomass hydrolysate using marine Rhodococcus sp, YHY01", Bioresour. Technol, 233, pp.99~109 (2017)
- Zaldivar J, "Effect of organic acids on the growth and fermentation of ethanologenic Escherichia coli LY01", Biotechnol. Bioeng, 66, pp.203~210 (1999)
- 송헌석, "L-Glycine Alleviates Furfural-Induced Growth Inhibition during Isobutanol Production in Escherichia coli", Journal of Microbiology and Biotechnology, 27(12), pp.2165~2172 (2017), 한국미생물·생명공학회
- Nieves LM, "Engineering sugar utilization and microbial tolerance toward lignocellulose conversion", Front. Bioeng. Biotechnol, 3, pp.17~ (2015)
- Moon J, "Direct enzyme assay evidence confirms aldehyde reductase function of Ydr541cp and Ygl039wp from Saccharomyces cerevisiae", Yeast, 32, pp.399~407 (2015)
- Han M-J, "Proteome analysis of metabolically engineeredescherichia coli producing poly(3-hydroxybutyrate)", J Bacteriol, 183, pp.301~308 (2001)
- Wang Q, "Complete PHB mobilization in Escherichia coli enhances the s tress to lerance : a potential biotechnological application", Microb. Cell Fact, 8, pp.47~ (2009)
- James BW, "Poly-3-hydroxybutyrate in Legionella pneumophila, an energy source for survival in low-nutrient environments, Appl. Environ Molecular biology series 3. Tools of molecular biology: gene cloning", Microbiol, 65, pp.822~827 (1999)
- Yang YH, "Improved detergent-based recovery of polyhydroxyalkanoates(PHAs)", Biotechnology Lett, 33, pp.937~942 (2011)