본문 바로가기
KERD
논문KCI 등재

Metabolic Engineering of Escherichia coli for Production of Polyhydroxyalkanoates with Hydroxyvaleric Acid Derived from Levulinic Acid

이성국|Kim Doyun
한국미생물·생명공학회 (The Korean Society For Microbiology And Biotechnology)|2022.01|Journal of Microbiology and Biotechnology|제32권 제1호|pp. 110-116|DOI 10.4014/jmb.2108.08016

2

조회

0

다운로드

0

인용

0

참고문헌

키워드

Escherichia coliLevulinic acid
주제분류생물학

초록

Polyhydroxyalkanoates (PHAs) are emerging as alternatives to plastics by replacing fossil fuels with renewable raw substrates. Herein, we present the construction of engineered Escherichia coli strains to produce short-chain-length PHAs (scl-PHAs), including the monomers 4-hydroxyvalerate (4HV) and 3-hydroxyvalerate (3HV) produced from levulinic acid (LA). First, an E. coli strain expressing genes (lvaEDABC) from the LA metabolic pathway of Pseudomonas putida KT2440 was constructed to generate 4HV-CoA and 3HV-CoA. Second, both PhaAB enzymes from Cupriavidus necator H16 were expressed to supply 3-hydroxybutyrate (3HB)-CoA from acetyl-CoA. Finally, PHA synthase (PhaCCv) from Chromobacterium violaceum was introduced for the subsequent polymerization of these three monomers. The resulting E. coli strains produced four PHAs (w/w% of dry cell weight): 9.1 wt% P(4HV), 1.7 wt% P(3HV-co-4HV), 24.2 wt% P(3HB-co-4HV), and 35.6 wt% P(3HB-co-3HV-co-4HV).

Abstract

Polyhydroxyalkanoates (PHAs) are emerging as alternatives to plastics by replacing fossil fuels with renewable raw substrates. Herein, we present the construction of engineered Escherichia coli strains to produce short-chain-length PHAs (scl-PHAs), including the monomers 4-hydroxyvalerate (4HV) and 3-hydroxyvalerate (3HV) produced from levulinic acid (LA). First, an E. coli strain expressing genes (lvaEDABC) from the LA metabolic pathway of Pseudomonas putida KT2440 was constructed to generate 4HV-CoA and 3HV-CoA. Second, both PhaAB enzymes from Cupriavidus necator H16 were expressed to supply 3-hydroxybutyrate (3HB)-CoA from acetyl-CoA. Finally, PHA synthase (PhaCCv) from Chromobacterium violaceum was introduced for the subsequent polymerization of these three monomers. The resulting E. coli strains produced four PHAs (w/w% of dry cell weight): 9.1 wt% P(4HV), 1.7 wt% P(3HV-co-4HV), 24.2 wt% P(3HB-co-4HV), and 35.6 wt% P(3HB-co-3HV-co-4HV).

참고문헌 (42)

  1. Reinecke F, "Ralstonia eutropha strain H16 as model organism for PHA metabolism and for biotechnological production of technically interesting biopolymers", Microb. Physiol, 16, pp.91~108 (2009)
  2. Jian Yu, "Microbial Production of Bioplastics from Renewable Resources", Bioprocessing for Value-Added Products from Renewable Resources, pp.585~610 (2007), Elsevier[DOI]
  3. Pettinari MJ, "Poly(3-hydroxybutyrate)synthesis genes in Azotobacter sp", strain FA8. Appl. Environ. Microbiol. 6, 7, pp.5331-5334~ (2001)
  4. Lütke-Eversloh T, "Microbial polythioesters", Macromol. Biosci, 4, pp.165~174 (2004)
  5. Napper IE, "Environmental deterioration of biodegradable, oxo-biodegradable, compostable, and conventional plastic carrier bags in the sea, soil, and open-air over a 3-year period", Environ. Sci. Technol, 53, pp.4775~4783 (2019)
  6. Bhatia SK, "Bioconversion of plant biomass hydrolysate into bioplastic(polyhydroxyalkanoates)using Ralstonia eutropha 5119", Bioresour. Technol, 271, pp.306~315 (2019)
  7. Lemoigne M, "Products of dehydration and of polymerization of β-hydroxybutyric acid", Bull. Chem. Soc. Japan, 8, pp.770~782 (1926)
  8. Muzaiyanah AR, "Studies on the microbial synthesis and characterization of polyhydroxyalkanoates containing 4-hydroxyvalerate using γ-valerolactone", Appl. Biochem. Biotechnol, 170, pp.1194~1215 (2013)
  9. Raza ZA, "Polyhydroxyalkanoates : Characteristics, production, recent developments and applications", Int. Biodeterior. Biodegradation, 126, pp.45~56 (2018)
  10. Możejko-Ciesielska J, "Bacterial polyhydroxyalkanoates : Still fabulous?", Microbiol. Res, 192, pp.271~282 (2016)
  11. Sheu D-S, "Cultivation temperature modulated the monomer composition and polymer properties of polyhydroxyalkanoate synthesized by Cupriavidus sp. L7L from levulinate as sole carbon source", Int. J. Biol. Macromol, 8, pp.1558~1564 (2018)
  12. Gahlawat G, "Valorization of waste glycerol for the production of poly(3-hydroxybutyrate)and poly(3-hydroxybutyrate-co-3-hydroxyvalerate)copolymer by Cupriavidus necator and extraction in a sustainable manner", Bioresour. Technol, 243, pp.492~501 (2017)
  13. Schmack G, "Biotechnological production and characterization of polyesters containing 4-hydroxyvaleric acid and medium-chain-length hydroxyalkanoic acids", Macromolecules, 31, pp.644~649 (1998)
  14. Lee W-H, "Biosynthesis of polyhydroxyalkanoate copolymers from mixtures of plant oils and 3-hydroxyvalerate precursors", Bioresour. Technol, 99, pp.6844~6851 (2008)
  15. Valentin HE, "Accumulation of poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid-co-4-hydroxyvaleric acid)by mutants and recombinant strains of Alcaligenes eutrophus", J. Environ. Polymer Degradation, 3, pp.169~175 (1995)
  16. Koller M, "Study on the effect of levulinic acid on whey-based biosynthesis of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)by Hydrogenophaga pseudoflava", Appl. Food Biotechnol, 4, pp.65~78 (2017)
  17. Novackova I, "Adaptation of Cupriavidus necator to levulinic acid for enhanced production of P(3HB-co-3HV)copolyesters", Biochem. Eng. J, 151, pp.107350~ (2019)
  18. Cha D, "Metabolic engineering of Pseudomonas putida for the production of various types of short-chain-length polyhydroxyalkanoates from levulinic acid", Bioresour. Technol, 309, pp.123332~ (2020)
  19. Bozell JJ, "Production of levulinic acid and use as a platform chemical for derived products", Resour. Conserv. Recycl, 28, pp.227~239 (2000)
  20. Favaro L, "Improving polyhydroxyalkanoate production from inexpensive carbon sources by genetic approaches : a review", Biofuels Bioprod. Biorefining, 13, pp.208~227 (2019)
  21. Kidwell J, "Regulated expression of the Alcaligenes eutrophus pha biosynthesis genes in Escherichia coli", Appl. Environ. Microbiol, 61, pp.1391~1398 (1995)
  22. Langenbach S, "Functional expression of the PHA synthase gene phaC1 from Pseudomonas aeruginosa in Escherichia coli results in poly(3-hydroxyalkanoate)synthesis", FEMS Microbiol. Lett, 150, pp.303~309 (1997)
  23. Ren Q, "Properties of engineered poly-3-hydroxyalkanoates produced in recombinant Escherichia coli strains", Appl. Environ. Microbiol, 66, pp.1311~1320 (2000)
  24. Ren Q, "Expression of PHA polymerase genes of Pseudomonas putida in Escherichia coli and its effect on PHA formation", Antonie Van Leeuwenhoek, 87, pp.91~100 (2005)
  25. Wang Q, "Complete PHB mobilization in Escherichia coli enhances the stress tolerance : a potential biotechnological application", Microb. Cell Fact, 8, pp.1~9 (2009)
  26. Rand JM, "A metabolic pathway for catabolizing levulinic acid in bacteria", Nat. Microbiol, 2, pp.1624~1634 (2017)
  27. Lee TS, "BglBrick vectors and datasheets : A synthetic biology platform for gene expression", J. Biol. Eng, 5, pp.12~ (2011)
  28. Gibson DG, "Enzymatic assembly of DNA molecules up to several hundred kilobases", Nat. Methods, 6, pp.343~345 (2009)
  29. Reis AC, "An automated model test system for systematic development and improvement of gene expression models", ACS Synth. Biol, 9, pp.3145~3156 (2020)
  30. Gorenflo V, "Development of a process for the biotechnological large-scale production of 4-hydroxyvalerate-containing polyesters and characterization of their physical and mechanical properties", Biomacromolecules, 2, pp.45~57 (2001)
  31. Juengert JR, "Determination of polyhydroxybutyrate(PHB)content in Ralstonia eutropha using gas chromatography and nile red staining", Bio Protoc, 8, pp.e2748~ (2018)
  32. Peña C, "Biotechnological strategies to improve production of microbial poly-(3-hydroxybutyrate) : a review of recent research work", Microb. Biotechnol, 7, pp.278~293 (2014)
  33. Matsumoto Ki, "A new pathway for poly(3-hydroxybutyrate)production in Escherichia coli and Corynebacterium glutamicum by functional expression of a new acetoacetyl-coenzyme A synthase", Biosci. Biotechnol. Biochem, 75, pp.364~366 (2011)
  34. Snell KD, "YfcX enables medium-chain-length poly(3-hydroxyalkanoate)formation from fatty acids in recombinant Escherichia coli fadB strains", J. Bacteriol, 184, pp.5696~5705 (2002)
  35. Liu S-J, "A novel genetically engineered pathway for synthesis of poly(hydroxyalkanoic acids)in Escherichia coli", Appl. Environ. Microbiol, 66, pp.739~743 (2000)
  36. Kim BS, "Production of poly(3-hydroxybutyrate)from inexpensive substrates", Enzyme Microb. Technol, 27, pp.774~777 (2000)
  37. Kim D, "High-level production of 4-hydroxyvalerate from levulinic acid via whole-cell biotransformation decoupled from cell metabolism", J. Agric. Food Chem, 67, pp.10678~10684 (2019)
  38. Steinbüchel A, "Synthesis and production of poly(3-hydroxyvaleric acid)homopolyester by Chromobacterium violaceum", Appl. Microbiol. Biotechnol, 39, pp.443~449 (1993)
  39. Tripathi L, "Pseudomonas putida KT2442 as a platform for the biosynthesis of polyhydroxyalkanoates with adjustable monomer contents and compositions", Bioresour. Technol, 142, pp.225~231 (2013)
  40. Aldor IS, "Process design for microbial plastic factories : metabolic engineering of polyhydroxyalkanoates", Curr. Opin. Biotechnol, 14, pp.475~483 (2003)
  41. Blattner FR, "The complete genome sequence of Escherichia coli K-12", Science, 277, pp.1453~1462 (1997)
  42. Durfee T, "The complete genome sequence of Escherichia coli DH10b : insights into the biology of a laboratory workhorse", J. Bacteriol, 190, pp.2597~2606 (2008)

관련 논문AI추천

Increased Tolerance to Furfural by Introduction of Polyhydroxybutyrate Synthetic Genes to Escherichia coli고병준, 문유미, 박예림, 박준영, 송헌석, 양수연, 양영헌, 이주희, 정혜림, 최태림, Ranjit Gurav, Shashi Kant Bhatia한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2019 · 제29권 제5호A Genetically Encoded Biosensor for the Detection of Levulinic Acid김희택, Kim Haseong, Kim Seong Keun, Kim Soo Jung, Kim Tae Hyun, Kwon Kil Koang, Lee Dae-Hee, Lee Hyewon, Lee Seung-Goo, Rha Eugene, Shin Jonghyeok, Sung Bong-Hyun, Woo Seung-Gyun, Yoo Byeong Hyeon한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2023 · 제33권 제4호Validating a Xylose Regulator to Increase Polyhydroxybutyrate Production for Utilizing Mixed Sugars from Lignocellulosic Biomass Using Escherichia coli양영헌, Bhatia Shashi Kant, Hwang Jeong Hyeon, Kim Hyun Jin, Lee Hong-Ju, Lee Sang-Ho, Oh Suk-Jin, Seo Seung-Oh, Shin Nara한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2024 · 제34권 제3호The Halophilic Bacterium Paracoccus haeundaensis for the Production of Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) from Single Carbon Sources남수완, 전용재, Burns Brendan, Jeong Gwi-Taek, Jin Deok Hyeon, Kim Seon Min, Lee Hye In, Nam Seung Won한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2024 · 제34권 제1호Pliot 규모에서의 재조합 대장균을 이용한(R)-3-Hydroxybutyric acid 생산이상엽, 이승환, 최성준, 최종일한국미생물·생명공학회 · 한국미생물·생명공학회지2004 · 제32권 제3호Engineering Escherichia coli for Anaerobic Succinate Fermentation Using Corn Stover Hydrolysate as a SubstrateDong Yali, Yang Haining한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2025 · 제35권

이 간행물의 다른 논문 (14건)

COVID-19 Drug Development김승택pp. 1-5A Brucella Omp16 Conditional Deletion Strain Is Attenuated in BALB/c MiceWang Aihua, Jin Yaping, Zhou Dong, Zhang Guangdong, Li Junmei, Zheng Weifang, Fang Jiaoyang, Zhi Feijiepp. 6-14A Newly Synthesized Flavone from Luteolin Escapes from COMT-Catalyzed Methylation and Inhibits Lipopolysaccharide-Induced Inflammation in RAW264.7 Macrophages via JNK, p38 and NF-κB Signaling PathwaysSun Zhi-qin, Xin Yang, Ye Lin, Wu Zhi-yuan, Sun Hai-jian, Huang De-jianpp. 15-26Novel Polyhydroxybutyrate-Degrading Activity of the Microbulbifer Genus as Confirmed by Microbulbifer sp. SOL03 from the Marine Environment양영헌, Ham Sion, Suha Min Ju, Kim Sang Hyun, Gurav Ranjit, Lee Hong-Ju, Cho Jang Yeon, Park Sol Lee, BHATIA SHASHI KANT, Kim Su Hyun, Park Kyungmoon, Park See-Hyoung, 김윤곤pp. 27-36Myricetin Disturbs the Cell Wall Integrity and Increases the Membrane Permeability of Candida albicans이흥식, 김연희pp. 37-45
원문 보기