흰쥐의 궁둥신경 압궤손상 후 운동훈련이 근육 재생에 미치는 영향에 관한 조직화학적 연구
Effect of Exercise Training on Histochemical Muscle Recovery after Sciatic Nerve Crush in Rat
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
말초신경병증에서 운동이 뼈대근육 재생에 미치는 영향에 대해 논란이 많다. 본 연구의 목적은 흰쥐의 궁둥신경에 압궤손상을 준 뒤 일정기간 트레드밀에서 달리기운동을 시켜 운동이 근육재생에 효과가 있는지를 밝힐 목적으로 시행하였다. 1개월(체중 150~180 g)된 수컷 Sprague-Dawley계 흰쥐의 궁둥신경을 노출시킨 다음 지혈집게로 3분간 압궤손상을 주었다. 각 실험군 별로 하루 60분 씩 주 5회 운동을 시켰으며, 트레드밀의 경사도는 10도, 속도는 20 m/분이었다. 궁둥신경 압궤손상 후 12일(5일간 운동), 19일(10일간 운동), 26일(15일간 운동), 33일(20일간 운동) 및 61일(40일간 운동)에 흰쥐를 희생시켜 장딴지근을 절취하여 냉동절편을 만들었으며 근육섬유에 대한 느린 미오신(slow myosin) 면역조직화학적 염색과 NADH-TR 효소조직화학 염색을 통상적인 절차에 따라 시행하였다. 압궤손상 후 12일째와 19일째에서 실험군의 흰쥐들이 대조군의 흰쥐보다 근육섬유의 위축이 심하였고, 33일째에서 실험군의 흰쥐들은 각진 모양의 근육 섬유들이 관찰되었으며, 61일째에서 정상근육에 가깝게 회복되었으나 대조군의 흰쥐는 그렇지 못했다. 압궤손상 후 26일째 운동군에서 II형 섬유의 중심에서 재신경지배가 되는 것이 관찰되었다. 손상 후 33일째에는 거대한 II형 섬유들이 관찰되었고, 재신경지배근육의 특성이라 할 수 있는 동일 근육섬유 군집현상이 나타났고, 61일째에는 정상근육에 가까운 근육섬유의 조성비를 보였다. 대조군에서는 손상 후 33일째에 거대한 II형 섬유들이 보이기 시작하였으나, 61일째에는 정상근육에 이르지는 못하였다. 이상의 결과로 흰쥐의 궁둥신경에 압궤손상을 준 후 트레드밀을 이용한 달리기 운동은 근육재생에 효과적인 도움을 준다고 생각된다.
Abstract
It is constant controversy that exercise influence muscle regeneration in peripheral neuropathy. The aim of this experiment is to show that treadmill running exercise under well-controlled conditions is to improve of regeneration in rat gastrocnemius muscles after sciatic nerve crushing injury. Male Sprague-Dawley rats (1 month old, weight 150~180 g) were submitted to bouts of exercise on a treadmill up a 10 degrees decline and speed is 20 m/min for 60 min per day and gastrocnemius muscles were analysed at different exercise periods (5, 10, 15, 20 and 40 days) by immunohistochemistry in comparison with injured non-exercised muscles. Rats were sacrificed at 12th (5 days exercise), 19th (10 days exercise), 26th (15 days exercise), 33rd day (20 days exercise), 61st day (40 days exercise) after sciatic nerve crushing injury. It showed that type II myofibers (target fibers) on center area had reinnervation at sciatic nerve crush injury at 26th day in exercise rats, as at 33rd day appeared giant type II myofibers, myofibers grouping observed in regenerative muscle character, component ratio of closed normal muscle showed at 61st day. Giant type II myofibers showed at 33rd day in non-exercise rats, however did not nearly normal muscle at 61st day. Therefore we concluded that treadmill running exercise is able to improve regeneration processes in gastrocnemius muscles after sciatic nerve crushing injury of rats.
참고문헌 (34)
- Ausoni S, "Expression of myosin heavy chain isoforms in stimulated fast and slow rat muscles", J Neurosci, 10, pp.153~160 (1990)
- Bedford TG, "Maximum oxygen consumption of rats and its changes with various experimental procedures", J Appl Physiol, 47, pp.1278~1283 (1979)
- Best TM, "Muscle injury and repair", Phys Med Rehabil Clin N Am, 11, pp.251~266 (2000)
- Bomemann A, "Satellite cells as players and targets in normal and diseased muscle", Neuropediatrics, 30, pp.167~175 (1999)
- Brooke MH, "Muscle fiber types: how many & what kind", Arch Neruol, 23, pp.369~379 (1970)
- Brownson C, "Reciprocal changes in myosin isoform mRNAs of rabbit skeletal muscle in response to the initiation and cessation of chronic electrical stimulation", Muscle Nerve, 15, pp.694~700 (1992)
- Carlson BM, "The functional morphology of regenerating and transplanted mammalian muscles", J Physiol, 231, pp.57~58 (1973)
- Carter GT, "Rehabilitation management of peripheral neuropathy", Semin Neurol, 25, pp.229~237 (2005)
- Chung WJ, "Histochemical studies of skeletal muscles after crushing injury on sciatic nerve in rats", Yonsei Med J, 16, pp.358~372 (1983)
- Costill DL, "Skeletal muscle enzymes and fiber composition in male and female track athletes", J Appl Physiol, 40, pp.149~154 (1976)
- De Koning P, "Methods for producing a reproducible crush in the sciatic and tibial nerve of the rat and rapid and precise testing of return of sensory function: Beneficial effects of melanocortins", J Neurol Sci, 74, pp.237~246 (1986)
- Dobkin BH, "Training and exercise to drive poststroke recovery", Nat Clin Pract Neurol, 4, pp.76~85 (2008)
- Dubowitz V, "Pathology of experimentally re-innervated skeletal muscle", J Neurol Neurosurg Psychiatry, 30, pp.99~110 (1967)
- Garvin TP, "Effect of short-term exercise training on angiogenic growth factor gene reponse in rats", J Appl Physiol, 90, pp.1219~1226 (2001)
- Grounds MD, "Identification of skeletal muscle precusor cells in vivo by use of MyoD1 and myogenin probes", Cell Tissue Res, 267, pp.99~104 (1992)
- Herbison GJ, "Effect of overwork during reinnervation of rat muscle", Exp Neurol, 41, pp.1~14 (1973)
- Herbison GJ, "Muscle weight and protein content of rat skeletal muscle following sciatic nerve crush", Arch Phys Med Rehabil, 55, pp.241~246 (1974)
- Herbison GT, "Reinnervating rat skeletal muscle: effect of 35% grade treadmil exereise", Arch Phys Med Rehabil, 63, pp.313~316 (1982)
- Hughes JT, "Pathology of muscle", Major Probl Pathol, 4, pp.1~218 (1974)
- Irintchev A, "Effects on recovery of soleus and extensor digitorum longus muscle of prolonged wheel running during a repeated nerve damage", Neuroscience, 44, pp.515~519 (1991)
- Irintchev A, "Muscle damage and repair in volun- tarily running mice: strain and muscle differences", Cell Tissue Res, 249, pp.509~521 (1987)
- Jansson E, "Changes in muscle fiber type distribution in man after physical training. A sign of fiber type transformation", Acta Physiol Scan, 104, pp.235~237 (1978)
- Jaweed MM, "Denervation and reinnervation of fast and slow muscles. A histochemical study in rats", J Histochem Cytochem, 23, pp.808~827 (1975)
- Kadi F, "Structural changes in male trapezius muscle work-related myalgia", Acta Neuropathol, 95, pp.352~360 (1998)
- Kapoukranidou D, "Assessment motorneuron death during development following neonatal nerve crush and Mg2+ treatment", Med Sci Monit, 11, pp.BR373~BR379 (2005)
- Maley MA, "Extracellular matrix, growth factors, genetics: their influence on cell proliferation and myotube formation in primary cultures of adult mouse skeletal muscle", Exp Cell Res, 219, pp.169~179 (1995)
- McAllister RM, "A review of effects of hypothyroidism on vascular transport in skeletal muscle during exercise", Can J Appl Physiol, 22, pp.1~10 (1997)
- Nakamura Y, "Delayed functional recovery by vincristine after sciatic nerve crush injury : a mouse model of vincristine neurotoxicity", Neurosci Lett, 304, pp.5~8 (2001)
- Satin B, "Skeletal muscle adaptability: significance for metabolism and performance. Handbook of Physiology", pp.555~631 (1983), Am Physiol Soc
- Seo TB, "Induction of Cdc2 protein by sciatic nerve injury and upregulation by exercise in rat", Society for Neurosci Abstr, 618, pp.8~ (2004)
- Stockert BW, "Peripheral neuropathies, 3rd ed., Neurological rehabilitaion", pp.360~374 (1995), Mosby
- Sunderland S, "The anatomy and physiology of nerve injury", Muscle Nerve, 13, pp.771~784 (1990)
- van Meeteren NL, "The effect of exercise training on functional recovery after sciatic nerve crush in the rat", J Peripher Nerv Syst, 3, pp.277~282 (1998)
- Waller A, "Experimental on the section of the glossopharyngeal and hypoglossal nerves of the frog, and observations of the alternations produced thereby in the structure of their primitive fiber", Philos Trans R Soc London, 140, pp.423~429 (1850)