Association between Leukocyte Mitochondrial DNA Copy Number and Regular Exercise in Postmenopausal Women
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Background: Previous studies suggest that habitual exercise can improve skeletal mitochondrial function; however, to date, the association between exercise and mitochondrial function in peripheral leukocytes has not been report-ed. The aim of this study was to evaluate the relationship between regular exercise and mitochondrial function by measuring leukocyte mitochondrial DNA (mtDNA) copy number in postmenopausal women.Methods: This cross-sectional study included 144 relatively healthy, non-diabetic, non-smoking, postmenopausal women. Clinical parameters, including anthropometric measurements and cardio-metabolic parameters, were as-sessed. Regular exercise was defined as at least 150 minutes per week of moderate-intensity activity, or an equiva-lent combination of moderate and vigorous-intensity activity, over a duration of at least 6 months. Leukocyte mtD-NA copy numbers were measured using real-time polymerase chain reaction assays, and these were normalized to the -globin copy number to give the relative mtDNA copy number.Results: The mtDNA copy number of peripheral leukocytes was significantly greater in the exercise group (1.33±0.02) than in the no exercise group (1.05±0.02, P<0.01). Stepwise multiple regression analysis showed that regular exercise was independently associated with mtDNA copy number ( =0.25, P<0.01) after adjusting for the variables age, body mass index, waist-to-hip ratio, systolic and diastolic blood pressure, homeostasis model assess-ment of insulin resistance value, and levels of high-density lipoprotein cholesterol, triglycerides, and homocysteine.Conclusion: Regular exercise is associated with greater leukocyte mtDNA copy number in postmenopausal wom-en.
Abstract
Background: Previous studies suggest that habitual exercise can improve skeletal mitochondrial function; however, to date, the association between exercise and mitochondrial function in peripheral leukocytes has not been report-ed. The aim of this study was to evaluate the relationship between regular exercise and mitochondrial function by measuring leukocyte mitochondrial DNA (mtDNA) copy number in postmenopausal women.Methods: This cross-sectional study included 144 relatively healthy, non-diabetic, non-smoking, postmenopausal women. Clinical parameters, including anthropometric measurements and cardio-metabolic parameters, were as-sessed. Regular exercise was defined as at least 150 minutes per week of moderate-intensity activity, or an equiva-lent combination of moderate and vigorous-intensity activity, over a duration of at least 6 months. Leukocyte mtD-NA copy numbers were measured using real-time polymerase chain reaction assays, and these were normalized to the -globin copy number to give the relative mtDNA copy number.Results: The mtDNA copy number of peripheral leukocytes was significantly greater in the exercise group (1.33±0.02) than in the no exercise group (1.05±0.02, P<0.01). Stepwise multiple regression analysis showed that regular exercise was independently associated with mtDNA copy number ( =0.25, P<0.01) after adjusting for the variables age, body mass index, waist-to-hip ratio, systolic and diastolic blood pressure, homeostasis model assess-ment of insulin resistance value, and levels of high-density lipoprotein cholesterol, triglycerides, and homocysteine.Conclusion: Regular exercise is associated with greater leukocyte mtDNA copy number in postmenopausal wom-en.
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