Ptecticus tenebrifer의 Life Cycle에 따른 생물학적 활용
The Biological Utilization Value of Ptecticus tenebrifer's Life Cycle
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본 연구는 동애등에(Ptecticus tenebrifer)의 life cycle 의 전반적인 활용 가치에 관한 연구이다. 동애등에 유충, 탈피각, 성충을 얻기 위해 전용 생육 장치를 고안및 제작하였다. 1령 유충을 밥, 소고기, 돼지비계, 양배추에 각각 넣고 분해 정도를 파악한 결과 양배추를 제외하고 분해가 잘되었으며, 생성된 분변토에서 RcBr의생장은 저해되었다. 탈피각을 채취하여 폐건전지 수용액의 중금속 흡착 정도를 SEM-EDS로 측정한 결과 다공성 구조로 Zn2+, Mn2+을 효과적으로 흡착한다는 것을확인하였다. 또한 탈피각을 섞은 배양토에서 RcBr의 생장률이 높았으며, 중금속을 처리하였을 경우 생장률은감소하였지만, RcBr의 안토시아닌 색소 발현량이 증가하였다. 탈피각으로 합성한 키토산을 엽면시비하여RcBr의 줄기 길이와 엽면 너비를 확인한 결과 생장량이증가하였다. 성충을 통해 바이오 디젤을 제작하였으며, 약 63.3%의 수득률을 얻을 수 있었고, 제작한 바이오디젤의 열량은 0.35kcal로 향우 에너지원으로서의 활용은 어려울 것으로 판단된다.
Abstract
This study is about the overall utilization value of the life cycle of Ptecticus tenebrifer. A dedicated growth device was designed and manufactured to obtain larvae, molted shells, and adults of Ptecticus tenebrifer. The 1st instar larvae were placed in rice, beef, pork fat, and cabbage, respectively, and the degree of organic matter degradation was evaluated. As a result, all substrates except cabbage were efficiently decomposed by the larvae, and the growth of Rapid cycling Brassica rapa(RcBr) in the resulting feces-soil mixture was inhibited. The molted shells were collected and the degree of heavy metal adsorption in the spent battery solution was measured by Scanning electron microscope-Energy dispersive spectrometer (SEM-EDS). As a result, it was confirmed that Zn2+ and Mn2+ were effectively adsorbed by the porous structure. In addition, the growth rate of RcBr was high in the culture soil mixed with the molted shells, and when heavy metals were treated, the growth rate decreased, but the expression of anthocyanin pigment of RcBr increased. The growth amount was increased when the stem length and leaf width of RcBr were investigated by foliar application of chitosan synthesized from the molted shells. Biodiesel was produced through adult insects, and a yield of approximately 63.3% was obtained. The calorific value of the biodiesel produced is 0.35 kcal, making it difficult to use as an energy source in the future.