황색포도알균의 철억제성 세포벽 단백과 세포외 단백의 면역반응성
Immunoreactivity of Iron-Repressible Cell Wall Proteins and Exoproteins ofStaphylococcus aureus
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저자들은 본 연구에서 황색포도알균에 의한 패혈증 환자의 회복기 혈청을 이용하여 황색포도알균의 철억제성 세포벽 단백들과 세포외 단백들의 면역반응성을 관찰하고자 하였다. 전기영동과 Western blot을 실시한 결과 철이 결핍된 배지에서 발현된 몇 가지 철억제성 세포벽 단백들은 회복기 환자혈청과 반응시켰을 때 강한 면역반응성을 보였으나 철이 풍부한 배지에서 발현된 대부분의 세포벽 단백들은 면역반응성을 보이지 않거나 상대적으로 약한 면역반응성을 보였다. 몇 가지 세포외 단백들은 철이 결핍된 배지에서만 발현되었으며 회복기 환자혈청과 반응시켰을 때 상대적으로 강한 면역반응성을 보였다. 이러한 결과를 통해 철억제성 세포벽 단백들과 세포외 단백들이 철이 상대적으로 결핍된 사람체내에서 발현될 뿐 아니라 면역원성을 가지고 있음을 알 수 있다. 따라서 포도알균에 의한 감염질환의 병인론 연구에 철결핍배지의 사용을 추천한다.
Abstract
In the present study, we tried to investigate immunoreactivity of iron-repressible cell wall proteins and exoproteins of Staphylococcus aureus when the convalescent serum obtained from the patient with septicemia was applied. On SDS-PAGE and western blot analysis, several iron-repressible cell wall proteins expressed in iron-deficient BHI showed strong immunoreactivity, whereas no or relatively weak immunoreactivity was shown in iron-sufficient BHI. Several exoproteins were expressed only in iron-deficient BHI and these exoproteins showed strong immunoreactivity. These results indicate that several iron-repressible cell wall proteins and exoproteins are expressed and are immunogenic in vivo. Since in vivo state is an relatively iron-restricted condition, we recommend the use of iron-deficient media for studies concerning pathogenicity of staphylococcal in human.
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