추출 조건에 따른 대황(Eisenia bicyclis) 추출물의 항산화 및 항균 활성
Antioxidant and Antimicrobial Activities of Eisenia bicyclis Extract under Different Extraction Conditions
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Marine-derived natural resources are emerging as promising alternatives to synthetic additives due to their high bioactive compound content and rich biodiversity. Among them, Eisenia bicyclis, a bioactive compound rich brown alga, has emerged as a potential functional material. E. bicyclis has been found to contain various bioactive metabolites (e.g., phlorotannins and their derivatives), with an efficacy and stability that could be influenced by the extraction conditions. Therefore, in this study, we aimed to evaluate how extraction conditions could affect the antioxidant and antimicrobial activities of E. bicyclis extracts. We prepared ethanol extracts at 50, 70, and 90°C for 4, 8, 12, and 24 h, and determined the antioxidant capacity using 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis-3-ethylbenzothiazoline- 6-sulphonic acid radical scavenging assays. We evaluated the antimicrobial activity by determining the minimum inhibitory and bactericidal concentrations against foodborne pathogens. Based both on antioxidant and antimicrobial evaluations, extraction at 70°C for 8–12 h an the optimal condition for preserving bioactivity while ensuring extraction efficiency and industrial applicability. These findings suggest that E. bicyclis extract obtained under controlled conditions could be used as a natural antioxidant and antimicrobial agent in the food, pharmaceutical, or cosmetic industries.
Abstract
Marine-derived natural resources are emerging as promising alternatives to synthetic additives due to their high bioactive compound content and rich biodiversity. Among them, Eisenia bicyclis, a bioactive compound rich brown alga, has emerged as a potential functional material. E. bicyclis has been found to contain various bioactive metabolites (e.g., phlorotannins and their derivatives), with an efficacy and stability that could be influenced by the extraction conditions. Therefore, in this study, we aimed to evaluate how extraction conditions could affect the antioxidant and antimicrobial activities of E. bicyclis extracts. We prepared ethanol extracts at 50, 70, and 90°C for 4, 8, 12, and 24 h, and determined the antioxidant capacity using 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis-3-ethylbenzothiazoline- 6-sulphonic acid radical scavenging assays. We evaluated the antimicrobial activity by determining the minimum inhibitory and bactericidal concentrations against foodborne pathogens. Based both on antioxidant and antimicrobial evaluations, extraction at 70°C for 8–12 h an the optimal condition for preserving bioactivity while ensuring extraction efficiency and industrial applicability. These findings suggest that E. bicyclis extract obtained under controlled conditions could be used as a natural antioxidant and antimicrobial agent in the food, pharmaceutical, or cosmetic industries.