Isolation and characterization of unrecorded yeasts species in the family Metschnikowiaceae and Bulleribasidiaceae in Korea
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
The goal of this study was to isolate and identify wild yeasts from soil samples. The 15 wild yeast strains were isolated from the soil samples collected in Pocheon city, Gyeonggi Province, Korea. Among them, four yeast stains were unrecorded, and 11 yeast stains were previously recorded in Korea. To identify wild yeasts, microbiological characteristics were observed by API 20C AUX kit. Pairwise sequence comparisons of the D1/D2 domain of the 26S rRNA were performed using Basic Local Alignment Search Tool (BLAST). Cell morphology of yeast strains was examined by phase contrast microscope. All strains were oval-shaped and polar budding and positive for assimilation of glucose, 2-keto-d-gluconate, N-acetyl-d-glucosamine, d-maltose and d-saccharose (sucrose). There is no official report that describes these four yeast species: one strain of the genus Kodamaea in the family Metschnikowiaceae and three strains of the Hannaella in the family Bulleribasidiaceae. Kodamaea ohmeri YI7, Hannaella kunmingensis YP355, Hannaella luteola YP230 and Hannaella oryzae YP366 were recorded in Korea, for the first time.
Abstract
The goal of this study was to isolate and identify wild yeasts from soil samples. The 15 wild yeast strains were isolated from the soil samples collected in Pocheon city, Gyeonggi Province, Korea. Among them, four yeast stains were unrecorded, and 11 yeast stains were previously recorded in Korea. To identify wild yeasts, microbiological characteristics were observed by API 20C AUX kit. Pairwise sequence comparisons of the D1/D2 domain of the 26S rRNA were performed using Basic Local Alignment Search Tool (BLAST). Cell morphology of yeast strains was examined by phase contrast microscope. All strains were oval-shaped and polar budding and positive for assimilation of glucose, 2-keto-d-gluconate, N-acetyl-d-glucosamine, d-maltose and d-saccharose (sucrose). There is no official report that describes these four yeast species: one strain of the genus Kodamaea in the family Metschnikowiaceae and three strains of the Hannaella in the family Bulleribasidiaceae. Kodamaea ohmeri YI7, Hannaella kunmingensis YP355, Hannaella luteola YP230 and Hannaella oryzae YP366 were recorded in Korea, for the first time.
참고문헌 (14)
- Altschul, S. F., "Gapped BLAST and PSI-BLAST : a new generation of protein database search programs", Nucleic Acids Res, 25(17), pp.3389~3402 (1997)
- Felsenstein, J., "Confidence limits on phylogenies : an approach using the bootstrap", Evolution, 39(4), pp.783~791 (1985)
- Freitas, L. F., "Kodamaea transpacifica f. a., sp. nov., a yeast species isolated from ephemeral flowers and insects in the Galapagos Islands and Malaysia : further evidence for ancient human transpacific contacts", Int J Syst Evol Microbiol, 63(Pt 11), pp.4324~4329 (2013)
- Gao, W. L., "Kodamaea neixiangensis f.a., sp. nov. and Kodamaea jinghongensis f.a., sp. nov., two yeast species isolated from rotting wood", Int J Syst Evol Microbiol, 67(9), pp.3358~3362 (2017)
- Han, L., "Hannaella dianchiensis sp. nov., a basidiomycetous yeast species isolated from lake water", Int J Syst Evol Microbiol, 67(6), pp.2014~2018 (2017)
- Kaewwichian, R., "Hannaella siamensis sp. nov. and Hannaella phetchabunensis sp. nov., two new anamorphic basidiomycetous yeast species isolated from plants", Int J Syst Evol Microbiol, 65(Pt 4), pp.1297~1303 (2015)
- Kimura, M., "A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences", J Mol Evol, 16(2), pp.111~120 (1980)
- Kurtzman, C. P., "Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit(26S)ribosomal DNA partial sequences", Antonie van Leeuwenhoek, 73(4), pp.331~371 (1998)
- Lachance, M., "The Yeasts", pp.483~490 (1999)
- Landell, M. F., "Hannaella pagnoccae sp. nov., a tremellaceous yeast species isolated from plants and soil", Int J Syst Evol Microbiol, 64(Pt 6), pp.1970~1977 (2014)
- Larkin, M. A., "Clustal W and Clustal X version 2. 0", Bioinformatics, 23(21), pp.2947~2948 (2007)
- Saitou, N., "The neighbour-joining method : a new method for reconstructing phylogenetic trees", Mol Biol Evol, 4(4), pp.406~425 (1987)
- Surussawadee, J., "Hannaella phyllophila sp. nov., a basidiomycetous yeast species associated with plants in Thailand and Taiwan", Int J Syst Evol Microbiol, 65(7), pp.2135~2140 (2015)
- Wang, Q. M., "Molecular phylogeny of basidiomycetous yeasts in the Cryptococcus luteolus lineage(Tremellales)based on nuclear rRNA and mitochondrial cytochrome b gene sequence analyses : proposal of Derxomyces gen. nov. and Hannaella gen. nov., and description of eight novel Derxomyces species", FEMS Yeast Res, 8(5), pp.799~814 (2008)