Isolation and characterization of two unrecorded yeast species in the order Filobasidiales
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초록
The purpose of this study was to isolate and identify wild yeasts from soil samples collected in Daegu and Cheongju city, Republic of Korea. To identify the wild yeast strains, pairwise sequence comparisons of D1/D2 region of the 26S rRNA gene sequence were done using Basic Local Alignment Search Tool (BLAST). The cell morphologies were observed by phase contrast microscope and assimilation test are done using API 20C AUX kit. All strains were assigned to the phylum Basidiomycota. Among 13 strains, 11 strains were previously reported, but two strains were unreported from the Republic of Korea. The two unrecorded yeast strains, GW1-3 and PG1-1-10C, belong to the genus Solicoccozyma (family Piskurozymaceae, order Filobasidiales, class Tremellomycetes). The two strains had oval-shaped and polar budding cells. This research showed the morphological and biochemical properties of the two unreported yeast species that had not officially reported in Korea.
Abstract
The purpose of this study was to isolate and identify wild yeasts from soil samples collected in Daegu and Cheongju city, Republic of Korea. To identify the wild yeast strains, pairwise sequence comparisons of D1/D2 region of the 26S rRNA gene sequence were done using Basic Local Alignment Search Tool (BLAST). The cell morphologies were observed by phase contrast microscope and assimilation test are done using API 20C AUX kit. All strains were assigned to the phylum Basidiomycota. Among 13 strains, 11 strains were previously reported, but two strains were unreported from the Republic of Korea. The two unrecorded yeast strains, GW1-3 and PG1-1-10C, belong to the genus Solicoccozyma (family Piskurozymaceae, order Filobasidiales, class Tremellomycetes). The two strains had oval-shaped and polar budding cells. This research showed the morphological and biochemical properties of the two unreported yeast species that had not officially reported in Korea.
참고문헌 (7)
- Altschul, S. F., "Gapped BLAST and PSI-BLAST : a new generation of protein database search programs", Nucleic Acids Res, 25(17), pp.3389~3402 (1997)
- Felsenstein, J., "Confidence limits on phylogenies : an approach using the bootstrap", Evolution, 39(4), pp.783~791 (1985)
- Kimura, M., "The neutral theory of molecular evolution" (1983), Cambridge University Press
- Kurtzman, C. P., "Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences", Antonie van Leeuwenhoek, 73(4), pp.331~371 (1998)
- Li, A. H., "Heterocephalacria sinensis sp. nov., Phaeotremella lacus sp. nov. and Solicoccozyma aquatica sp. nov., three novel basidiomycetous yeast species isolated from crater lakes", International Journal of Systematic and Evolutionary Microbiology, 69(12), pp.3728~3739 (2019)
- Liu, X. Z., "Towards an integrated phylogenetic classification of the Tremellomycetes", Studies in Mycology, 81(1), pp.85~147 (2015)
- Tamura, K., "MEGA11 : Mole-cular Evolutionary Genetics Analysis Version 11", Mol Biol Evol, 38, pp.3022~3027 (2021)