Postprandial hypoglycemic effect of mulberry leaf in Goto-Kakizaki rats and counterpart control Wistar rats
4
조회
0
다운로드
7
인용
0
참고문헌
키워드
초록
Postprandial hypoglycemic effect of mulberry leaf (Morus alba L.) was compared in two animal models: Goto-Kakizaki (GK) rats, a spontaneousnon-obese animal model for type II diabetes, and their counterpart control Wistar rats. First, the effect of a single oral administration of mulberryleaf aqueous extract (MLE) on postprandial glucose responses was determined using maltose or glucose as substrate. With maltose-loading, MLEreduced peak responses of blood glucose significantly in both GK and Wistar rats (P < 0.05), supporting the inhibition of α-glucosidase by MLEin the small intestine. With glucose-loading, MLE also significantly reduced blood glucose concentrations, measured at 30 min, in both animalmodels (P < 0.01), proposing the inhibition of glucose transport by MLE. Next, dried mulberry leaf powder (MLP) was administered for 8 weeksby inclusion in the diet. By MLP administration, fasting blood glucose was significantly reduced at weeks 4 and 5 (P < 0.05), but then returnedto values that were similar to those of the control at the end of experimental period in GK rats. Insulin, HOMA-IR, C-reactive protein, and triglyceridestended to be decreased by MLP treatment in GK rats. All other biochemical parameters were not changed by MLP administration in GK rats. Collectively, these findings support that MLE has significant postprandial hypoglycemic effect in both non-obese diabetic and healthy animals, whichmay be beneficial as food supplement to manage postprandial blood glucose. Inhibitions of glucose transport as well as α-glucosidase in the smallintestine were suggested as possible mechanisms related with the postprandial hypoglycemic effect of MLE.
Abstract
Postprandial hypoglycemic effect of mulberry leaf (Morus alba L.) was compared in two animal models: Goto-Kakizaki (GK) rats, a spontaneousnon-obese animal model for type II diabetes, and their counterpart control Wistar rats. First, the effect of a single oral administration of mulberryleaf aqueous extract (MLE) on postprandial glucose responses was determined using maltose or glucose as substrate. With maltose-loading, MLEreduced peak responses of blood glucose significantly in both GK and Wistar rats (P < 0.05), supporting the inhibition of α-glucosidase by MLEin the small intestine. With glucose-loading, MLE also significantly reduced blood glucose concentrations, measured at 30 min, in both animalmodels (P < 0.01), proposing the inhibition of glucose transport by MLE. Next, dried mulberry leaf powder (MLP) was administered for 8 weeksby inclusion in the diet. By MLP administration, fasting blood glucose was significantly reduced at weeks 4 and 5 (P < 0.05), but then returnedto values that were similar to those of the control at the end of experimental period in GK rats. Insulin, HOMA-IR, C-reactive protein, and triglyceridestended to be decreased by MLP treatment in GK rats. All other biochemical parameters were not changed by MLP administration in GK rats. Collectively, these findings support that MLE has significant postprandial hypoglycemic effect in both non-obese diabetic and healthy animals, whichmay be beneficial as food supplement to manage postprandial blood glucose. Inhibitions of glucose transport as well as α-glucosidase in the smallintestine were suggested as possible mechanisms related with the postprandial hypoglycemic effect of MLE.
참고문헌 (27)
- Andallu B, "Antioxidant role of mulberry (Morus indica L. cv. Anantha) leaves in streptozotocin-diabetic rats", Clinica Chimica Acta, 338, pp.3~10 (2003)
- B. Andallu, "Gluconeogenic Substrates and Hepatic Gluconeogenic Enzymes in Streptozotocin-Diabetic Rats: Effect of Mulberry (Morus indica L.) Leaves", Journal of Medicinal Food, 10(1), pp.41~48 (2007), 한국식품영양과학회
- Asano N, "Polyhydroxy alkaloids islated from mulberry trees (Morus alba L.) and silkworms (Bombyx mori L.)", J Agric Food Chem, 49, pp.4208~4213 (2001)
- Avignon A, "Nonfasting plasma glucose is better marker of diabetic control than fasting plasma glucose in type 2 diabetes", Diabetes Care, 20, pp.1822~1826 (1997)
- Chan JC, "Diabetes in Asia: epidemiology, risk factors, and pathophysiology", J Am Med Assoc, 301, pp.2129~2140 (2009)
- Goda T, "Effects of miglitol, an α-glucosidase inhibitor, on glycaemic status and histopathological changes in islets in non-obese, non-insulin-dependent diabetic Goto-Kakizaki rats", Br J Nutr, 98, pp.702~710 (2007)
- Goto Y, "Abnormal insulin secretion and glucose metabolism in pancreatic islets from the spontaneously diabetic GK rat", Diabetologia, 36, pp.3~8 (1993)
- Igarashi K, "Effect of dietary catechins on glucose tolerance, blood pressure and oxidation statues in Goto-Kakizaki rats", J Nutr Sci Vitaminol, 53, pp.496~500 (2007)
- Janssen U, "Rodent models of nephropathy associated with type 2 diabetes", J Nephrol, 12, pp.159~172 (1999)
- Kim J, "Determination of 1-deoxynojirimycin in Morus alba L. leaves by derivatization with 9-fluorenylmethyl chloroformate followed by reversed-phase high-performance liquid chromatography", J Chromatogr A, 1002, pp.93~99 (2003)
- Kimura T, "Food-grade mulberry powder enriched with 1-deoxynojirimycin suppresses the elevation of postprandial blood glucose in humans", J Agric Food Chem, 55, pp.5869~5874 (2007)
- Kimura T, "Determination of 1-deoxynojirimycin in Mulberry leaves using hydrophilic interaction chromatography with evaporative light scattering detection", J Agric Food Chem, 52, pp.1415~1418 (2004)
- Kwon O, "Inhibition of the intestinal glucose transporter GLUT2 by flavonoids", FASEB J, 21, pp.366~377 (2007)
- 김정인, "Inhibitory activity of Euonymus alatus alpha-glucosidase in vitro and in vivo", Nutr Res Pract, 1(3), pp.184~188 (2007), 한국영양학회
- Miyahara C, "Inhibitory effects of mulberry leaf extract on postprandial hyperglycemia in normal rats", J Nutr Sci Vitaminol, 50, pp.161~164 (2004)
- Nakagawa K, "Occurrence of orally administered mulberry 1-Deoxynojirimycin in rat plasma", J Agric Food Chem, 55, pp.8928~8933 (2007)
- Nutrition Research Council, "Guide for the care and use of laboratory animals, 4th Edition" (1997), National Academy Press
- Oku T, "Inhibitory effects of extractives from leaves of Morus alba on human and rat small intestinal disaccharidase activity", Br J Nutr, 95, pp.933~938 (2006)
- Prosky L, "Determination of total dietary fiber in food and food products: Collaborative study", J Assoc Off Anal Chem, 67, pp.1044~1052 (1984)
- Prosky L, "Determination of insoluble and soluble dietary fiber in foods and food products: collaborative study", J AOAC Int, 75, pp.360~367 (1992)
- Rother KI, "Diabetes treatment-bridging the divide", N Engl J Med, 356, pp.1499~1501 (2007)
- Voss AA, "Imino sugars are potent agonists of the human glucose sensor SGLT3", Mol Pharmacol, 71, pp.628~634 (2007)
- Wheeler ML, "Carbohydrate issues: type and amount", J Am Diet Assoc, 108, pp.S34~S39 (2008)
- Wild S, "Global prevalence of diabetes: estimates for the year 2000 and projections for 2030", Diabetes Care, 27, pp.1047~1053 (2004)
- Wong JMW, "Carbohydrate digestibility and metabolic effects", J Nutr, 137, pp.2539S~2546S (2007)
- Yatsunami K, "The relationship between 1-deoxynojirimycin content and alpha-glucosidase inhibitory activity in leaves of 276 mulberry cultivars (Morus spp.)", J Nat Med, 62, pp.63~66 (2008)
- Zhou YP, "Deficiency of pyruvate dehydrogenase activity in pancreatic islets of diabetic GK rats", Endocrinology, 136, pp.3546~3551 (1995)