한국산 동백종자 초임계 추출물의 피부 항산화 및 항노화 연구
Anti-oxidant and Anti-aging Effect of Extracts from Seed of Camellia japonica L. by Supercritical Fluid Extraction
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Objectives : The aim of this study was to investigate the anti-oxidant and anti-aging effect of the seed of Camellia japonica L. extracted by supercritical CO2. Methods : The cell viability was performed by MTT assay. Nitric oxide (NO) production was performed by NO assay. The anti-oxidant effect was performed by beta-carotene bleaching assay and the intracellular proteome was analyzed expression of each 15 proteins by 2-D electrophoresis. Results : Fatty acid analysis of extract from Camellia japonica L. has shown oleic acid was 84%. And the antioxidant effect was about 186% compared with alpha-tocoperol(0.1%) by beta carotene bleaching assay. In 2D PAGE analysis, fifteen protein changes in five mechanisms which was collagen synthesis pathway, MMPs, ECM-cell interaction, cytokine, antioxidant enzymes were analyzed. Conclusions : On this study extract from seed of Camellia japonica L. by supercritical extraction could be used as a antioxidant and antiaging substance for the skin.
Abstract
Objectives : The aim of this study was to investigate the anti-oxidant and anti-aging effect of the seed of Camellia japonica L. extracted by supercritical CO2. Methods : The cell viability was performed by MTT assay. Nitric oxide (NO) production was performed by NO assay. The anti-oxidant effect was performed by beta-carotene bleaching assay and the intracellular proteome was analyzed expression of each 15 proteins by 2-D electrophoresis. Results : Fatty acid analysis of extract from Camellia japonica L. has shown oleic acid was 84%. And the antioxidant effect was about 186% compared with alpha-tocoperol(0.1%) by beta carotene bleaching assay. In 2D PAGE analysis, fifteen protein changes in five mechanisms which was collagen synthesis pathway, MMPs, ECM-cell interaction, cytokine, antioxidant enzymes were analyzed. Conclusions : On this study extract from seed of Camellia japonica L. by supercritical extraction could be used as a antioxidant and antiaging substance for the skin.
참고문헌 (28)
- Oh JH, "Intrinsicaging- and photoaging-dependent level changes of glycosaminoglycans and their correlation with water content in human skin", Journal of Dermatological Science, pp.192~201 (2011)
- Jung BS, "Dohae hyangyagdaesajeon", pp.402~403 (2003), Young Rim Sa
- 이숙영, "동백나무 잎과 꽃 추출물의 항미생물 활성 및 항산화 효과", 한국약용작물학회지, 13(3), pp.93~100 (2005), 한국약용작물학회
- Lee SJ, "Dohae Bonchogangmok", pp.410~415 (1975), Komoonsa
- Hwang DY, "Uijongsonig", pp.238~239 (1993), Yeogang
- Ko WS, "Text of Traditional Korean Dermatology & Surgery", pp.263~270 (2007), Seonu
- Yang WY, "Oxidative Stability and Sensory Evaluation of Camellia oil", KOREAN J. SOC. FOOD SCI., 12(3), pp.367~371 (1996)
- 김선민, "국내 자생 동백나무(Camellia japonica L.) 추출물의 항산화 및 항미생물 활성", 한국자원식물학회지, 17(3), pp.314~322 (2004), 한국자원식물학회
- Kang SK, "Proximate, Saponin and Amino Acid Comositions in Camellia Seeds and Defatted camellia Seeds", J. Korean Soc. Food Sci. Nurt, 27(2), pp.227~231 (1998)
- Kang SK, "Antimicrobial Activity of Defatted camellia Seeds Extract", J. Korean Soc. Food Sci. Nurt, 27(2), pp.232~238 (1998)
- KDA Textbook Editing Board, "Dermatology", pp.149~150 (2008), RyoMoonGak
- Ahn SK, "Common Skin Disease", pp.626~630 (2020), JN Medbook
- 곽병문, "한방약침소재로써 속수자 초임계추출물의 항산화 및 항노화에 대한 연구", Korean Journal of Acupuncture, 37(2), pp.88~96 (2020), 경락경혈학회[DOI]
- Ahn SK, "Common Skin Disease", pp.311~521 (2015), JN Medbook
- 박수남, "밤나무 잎 추출물의 항노화 활성 및 화장품에의 응용(Ⅰ)", 대한화장품학회지, 34(4), pp.259~268 (2008), 사단법인 대한화장품학회
- Fernandes R, "A Role for Prolyl 3-Hydroxylase 2 in Post-translational Modification of Fibrilforming Collagens", J Biol Chem, 286(35), pp.30662~30669 (2011)
- Daniele M, "Collagen Prolyl Hydroxylases Are Essential for Breast Cancer Metastasis", Cancer Res, 73(11), pp.3285~3296 (2013)
- Chiusaroli R, "Collagenase cleavage of type I collagen is essential for both basal and parathyroid hormone(PTH)", PTH-related peptide receptor-induced osteoclast activation and has differential effects on discrete bone compartments, 2003, pp.144, 4106~4116
- Alain M, "Comparative Effects of Interleukin-1 and Tumor Necrosis Factor-αon Collagen Production and Corresponding Procollagen mRNA Levels in Human Dermal Fibroblasts", Journal of Investigative Dermatology, 96(2), pp.243~249 (1991)
- Heo GC, "Junguioegwasanggwamyeongjeojipseong", pp.271~272 (1997), Huaxia publishing house
- 김희경, "황련이 화상조직에 미치는 항산화와 항염증 및 피부재생 효과", 대한한의학회지, 32(2), pp.1~13 (2011), 대한한의학회
- Guo HF, "Temporal changes in the cell population and wound healing-related gene expression in deep partial-thickness burn wound model", Biomedical Dermatology, 4(5), pp.1~8 (2020)
- O G., "Uijonggeumgam", pp.448~449 (1994), Daeseongmunhwasa
- KH Textbook Editing Board, "Herbology", pp.218~221 (2008), Young Rim Sa
- 이재근, "黃連이 구강암 세포에서의 세포자멸사에 미치는 영향", 한방안이비인후피부과학회지, 22(2), pp.50~59 (2009), 대한한방안이비인후피부과학회
- 이수경, "광화학적 뇌경색 백서 모델에서 황련의 항염증 및 운동기능 회복에 미치는 효과", 대한본초학회지, 24(1), pp.179~189 (2009), 대한본초학회
- Kim YJ, "Antioxidant Activity of Water-Extract from Coptis chinensis Franch", Korean journal of life science, 10(3), pp.241~246 (2000)
- 조일주, "마우스 대식세포주인 RAW 264.7 세포에서 蜈蚣의 항염증 효과", 대한본초학회지, 26(3), pp.23~29 (2011), 대한본초학회