역기하렌즈와 RGP렌즈 착용 후 가토각막에서 상피세포의 이동과 재분포에 관한 고찰
The Effect of RGP Lens and Reverse Geometry Lens on Redistribution of Corneal Epithelial Cell in Rabbit
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목적: 역기하렌즈의 착용이 가토각막에서 상피세포의 이동에 의한 재분포를 일으킨다는 가설을 검증해보고자 하였다. 대상과 방법: 가토 30마리 중 15마리의 우안은 RGP (rigid gas permeable)렌즈를, 나머지 15마리의 우안은 역기하렌즈를 착용시키고좌안을 대조안으로 하여 렌즈 착용 후 1, 3, 7, 10, 14일에 각각 3마리씩 희생하였다. Hematoxylin-Eosin 염색 후 각막의 중심부와 주변부의 상피세포층과 실질의 두께를 측정하였고, 각막상피세포의 이동과 관련된 표지자인 7-nitrobenz-2-ox-1,3-diazolylphallacidin을이용하여 면역형광염색 후 형광현미경을 이용하여 관찰하였다. 결과: 역기하렌즈와 RGP렌즈를 착용한 가토안의 각막 중심부와 주변부에서는 일관적인 두께 변화가 관찰되지 않았다. 면역형광염색 결과역기하렌즈를 착용한 가토안에서 RGP렌즈를 착용한 가토안이나 대조안보다 형광반응이 각막 전체에서 더 뚜렷하게 관찰되었고, 역기하렌즈를 착용한 가토안에서는 3일째, RGP렌즈를 착용한 가토안에서는 7일째 가장 강한 형광을 나타낸 뒤 점차 감소하는 경향이 나타났다. 결론: 이 연구의 동물실험에서 면역형광염색의 결과는 역기하렌즈를 착용하였을 경우 각막상피세포의 이동에 의한 재분포가 실제적으로 이루어지고 있음을 시사한다.
Abstract
Purpose: To investigate the migration and redistribution of rabbit corneal epithelial cells when wearing reverse geometrylens (RGL) or rigid gas permeable lens (RGP). Methods: In 30 rabbits, the right eyes were fitted with either RGL or RGP and the left eyes were untreated to serve ascontrols. The rabbits were sacrificed at 1, 3, 7, 10 and 14 days after lens fitting. The central and peripheral corneal thicknesseswere measured by microscope and the ratio of right to left corneal thickness was calculated to evaluate the characteristicsof change over time. By using the molecular probe 7-nitrobenz-2-ox-1,3-diazolylphallacidin (NBD phallacidin), thesamples were examined with light microscope to determine the migration and redistribution of epithelial cells in the rabbitcornea. Results: No consistent changes in the thickness of both central and peripheral corneal epithelium were found. The cornealepithelial cells of both eyes with RGL and RGP reacted positively to NBD phallacidin. The fluorescence was most increasedat day 3 of sacrifice in RGL cases and at day 7 in RGP cases, and then decreased in both cases. The corneal epitheliumof eyes with RGL exhibited marked increase in the intensity of fluorescence compared to the eyes with RGP. Conclusions: The corneal epithelium with RGL showed the strongest intensity of NBD phallacidin fluorescence. This resultsuggests that wearing RGL may induce the migration and redistribution of corneal epithelial cells.
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