콘택트렌즈용 다목적용액(MPS)의 세포증식저해 및실험용 토끼 각막에 미치는 영향
The Cytotoxicity and Effect on the Experimental Rabbit Cornea of Soft Contact Lens Multi-PurposeSolution(MPS)
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본 연구는 시중에서 유통되고 있는 콘택트렌즈 관리용액인 다목적용액 (multi-purpose solution, MPS)이 세포에미치는 증식저해정도와 실험용 토끼눈의 각막 상피 및내피조직에 미치는 손상정도를 비교 관찰하고자 시행하였다. MPS는 ReNu?? (Baush & Lomb, USA), Opti-freeexpress?? (Alcon, USA), Free-sol plus?? (Hanamedicon,Korea)를 사용하였다. 세포증식 저해율은 L929 세포주를배양 후 MTT assay로 검정하였고, 형태학적으로는 광학현미경과 Hematoxylin & Eosin staining 표본을 제작하여관찰하였다. In vivo실험은 백색 가토 9마리(18안)를 3군으로 분류하여 실험군인 좌안(9안)에는 각 MPS 제품을,대조군인 우안(9안)에는 보존제가 포함되지 않은 멸균생리식염수를 점안하였다. 일정기간 점안 후, 실험용 토끼눈의 각막표면을 Rose bengal로 염색하여 관찰하였고 각막상피 및 내피조직의 변화는 주사전자현미경 (scanningelectron microscopy, SEM)으로 관찰하였다. 세포증식 저해율은 각각 54, 73, 36%로 나타났으며, 형태학적 변화는L929 세포주의 일반적인 형태와 달리 타원형 또는 원형
Abstract
The purpose of this study is to find out how soft contact lens multi-purpose solution (MPS), often used formedical treatment, effects the inhibition on cell growth and research the result of using MPS, suspected todemage eye cells, on rabbit eye’s corneal epithelium and endothelium tissue. In this treatise, ReNu?? (Baush &Lomb, USA), Opti-free express?? (Alcon, USA), Free-sol plus?? (Hanamedicon, Korea) had been selectedamong the MPS. After culturing L929 cell line, cell growth inhibition rate was measured by MTT assay, andIn vivo experiment, 9 white rabbit eyes (18 eyes) were classified into 3 groups. The experimental group is lefteyes(9 eyes) of rabbit, and MPS were dropped; however, the control group, the right eyes(9 eyes), were onlyused a saline solution without preservatives. After the dropping within the period, the cornea surface of rabbitendothelium tissue scanning electron microscopy (SEM) has been used. As the result, the rate of cell growthinhibition was 54%, 73% and 36%, respectively. Morphological changes represented that the shape has beenchanged into oval or round shape and those are not considered as a common formation of L929 cell line. Whenpolygonal mosaic pattern of a corneal epithelium was disturbed in the picture taken by SEM; furthermore, theshape of the corneal endothelium was irregular.In conclusion, as we consider antimicrobial effect and the safety on living cells, it is necessary that weshould improve concentration of preservatives and study continuously to develop a new preservatives without atoxic effect on the cornea surface.