눈물 내 단백질 분석을 위한 눈물 수집 방법의 효용성 비교
Comparison of the Effectiveness between Sampling Methods for Protein Analysis of Tear Fluids
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목적: 결막낭으로부터 눈물을 수집하는 다양한 방법이 보고되었으나, 이들의 효과 및 단백질 수집 효율을 비교한 연구는 없었다. 본연구는 보고된 다양한 눈물 수집 방법을 환자의 순응도, 눈물의 양, 분리된 단백질 농도의 항목으로 평가하여 비교하고, 가장 효율적인 방법을 찾고자 하였다. 대상과 방법: 정상안 14안을 대상으로 쉬르머 종이, 모세관, 셀룰로스 막대, 재질과 밀도가 다른 세 종류의 안과용 스펀지−메로셀(MerocelⓇ), 케라셀(KeraCelⓇ) 그리고 웩셀(Weck-celⓇ)을 이용하여 휴지기 눈물을 수집하였다. 눈물을 흡수시킨 샘플을 원심분리시킨 후 각 방법별로 수집된 눈물의 양과 단백질 농도를 측정하였다. 또한 눈물을 수집하는 대상안에서 느끼는 불편감을 수치화하여검사의 순응도를 분석하였다. 결과: 각 눈물 수집 방법으로 분리된 눈물 부피는 평균 9.0 ± 1.1 μL였으며 각 군 간 유의한 차이가 없었다. 각 눈물 수집 방법으로분리된 단백질의 정량은 평균 5.3 ± 1.2 μg/μL였으며 메로셀에서 7.6 ± 0.61 μg/μL로 다른 군들에 비해 유의하게 많이 정량되었다(p ＜0.05). 순응도는 메로셀과 모세관이 순응도가 높았으며 케라셀이 가장 순응도가 떨어졌다. 결론: 메로셀을 이용하였을 때 가장 많은 양의 단백질을 분리하였으며, 대상안의 순응도가 높아 가장 효용성이 높고 임상적인 활용이용이한 눈물 수집 방법으로 생각된다.
Abstract
Purpose: Although various sampling methods of tears from conjunctival sac have been reported, no previous study compared their effectiveness or efficiency based on protein extraction. By comparing the compliance, volume and protein concentration of each tear sampling method, we searched for the most efficient tear collection method. Methods: Resting tear samples of 14 eyes of normal subjects were collected using Schirmer paper, capillary tube, cellolose acetate rod and 3 different ophthalmic sponges made of different materials and density (Merocel®, KeraCel® and Weck-Cel®). After centrifugation of the collected tear samples, the tear volume and protein concentration were measured for each method. Additionally, the compliance of each tear sampling method was analyzed by numerically representing the amount of discomfort experienced during resting tear collection. Results: The average volume retrieved by each tear sampling method was 9.0 ± 1.1 μL with no significant differences between groups. The average concentration of protein retrieved by each tear sampling method was 5.3 ± 1.2 μg/μL. Merocel® retrieved 7.6 ± 0.61 μg/μL, which was significantly higher than other sampling methods (p < 0.05). The compliance of Merocel® and the capillary tube were the highest, while KeraCel® showed the lowest compliance. Conclusions: Merocel® retrieved the highest amount of protein and showed high compliance and may be the most effective and easily applicable tear sampling method in clinical settings.
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