본문 바로가기
KERD
논문KCI 등재

Hepatic transcriptional changes in critical genes for gluconeogenesis following castration of bulls

2

조회

0

다운로드

2

인용

0

참고문헌

키워드

초록

Objective: This study was performed to understand transcriptional changes in the genes involved in gluconeogenesis and glycolysis pathways following castration of bulls. Methods: Twenty Korean bulls were weaned at average 3 months of age, and castrated at 6 months. Liver tissues were collected from bulls (n = 10) and steers (n = 10) of Korean cattle, and hepatic gene expression levels were measured using quantitative real-time polymerase chain reaction. We examined hepatic transcription levels of genes encoding enzymes for irreversible reactions in both gluconeogenesis and glycolysis as well as genes encoding enzymes for the utilization of several glucogenic substrates. Correlations between hepatic gene expression and carcass characteristics were performed to understand their associations. Results: Castration increased the mRNA (3.6 fold; p<0.01) and protein levels (1.4 fold; p< 0.05) of pyruvate carboxylase and mitochondrial phosphoenolpyruvate carboxykinase genes (1.7 fold; p<0.05). Hepatic mRNA levels of genes encoding the glycolysis enzymes were not changed by castration. Castration increased mRNA levels of both lactate dehydrogenase A (1.5 fold; p<0.05) and lactate dehydrogenase B (2.2 fold; p<0.01) genes for lactate utilization. Castration increased mRNA levels of glycerol kinase (2.7 fold; p<0.05) and glycerol-3-phosphate dehydrogenase 1 (1.5 fold; p<0.05) genes for glycerol utilization. Castration also increased mRNA levels of propionyl-CoA carboxylase beta (mitochondrial) (3.5 fold; p<0.01) and acyl-CoA synthetase short chain family member 3 (1.3 fold; p = 0.06) genes for propionate incorporation. Conclusion: Castration increases transcription levels of critical genes coding for enzymes involved in irreversible gluconeogenesis reactions from pyruvate to glucose and enzymes responsible for incorporation of glucogenic substrates including lactate, glycerol, and propionate. Hepatic gluconeogenic gene expression levels were associated with intramuscular fat deposition.

Abstract

Objective: This study was performed to understand transcriptional changes in the genes involved in gluconeogenesis and glycolysis pathways following castration of bulls. Methods: Twenty Korean bulls were weaned at average 3 months of age, and castrated at 6 months. Liver tissues were collected from bulls (n = 10) and steers (n = 10) of Korean cattle, and hepatic gene expression levels were measured using quantitative real-time polymerase chain reaction. We examined hepatic transcription levels of genes encoding enzymes for irreversible reactions in both gluconeogenesis and glycolysis as well as genes encoding enzymes for the utilization of several glucogenic substrates. Correlations between hepatic gene expression and carcass characteristics were performed to understand their associations. Results: Castration increased the mRNA (3.6 fold; p<0.01) and protein levels (1.4 fold; p< 0.05) of pyruvate carboxylase and mitochondrial phosphoenolpyruvate carboxykinase genes (1.7 fold; p<0.05). Hepatic mRNA levels of genes encoding the glycolysis enzymes were not changed by castration. Castration increased mRNA levels of both lactate dehydrogenase A (1.5 fold; p<0.05) and lactate dehydrogenase B (2.2 fold; p<0.01) genes for lactate utilization. Castration increased mRNA levels of glycerol kinase (2.7 fold; p<0.05) and glycerol-3-phosphate dehydrogenase 1 (1.5 fold; p<0.05) genes for glycerol utilization. Castration also increased mRNA levels of propionyl-CoA carboxylase beta (mitochondrial) (3.5 fold; p<0.01) and acyl-CoA synthetase short chain family member 3 (1.3 fold; p = 0.06) genes for propionate incorporation. Conclusion: Castration increases transcription levels of critical genes coding for enzymes involved in irreversible gluconeogenesis reactions from pyruvate to glucose and enzymes responsible for incorporation of glucogenic substrates including lactate, glycerol, and propionate. Hepatic gluconeogenic gene expression levels were associated with intramuscular fat deposition.

참고문헌 (36)

  1. Nafikov RA, "Carbohydrate and lipid metabolism in farm animals carbohydrate and lipid metabolism in farm animals", J Nutr, 137, pp.702~705 (2007)[DOI]
  2. Huntington GB, "Sites, rates, and limits of starch digestion and glucose metabolism in growing cattle", J Anim Sci, 84, pp.E14~E24 (2006)[DOI]
  3. Aschenbach JrR, "Gluconeogenesis in dairy cows: the secret of making sweet milk from sour dough", IUBMB Life, 62, pp.869~877 (2010)[DOI]
  4. Bong JJ, "Differential expression of genes associated with lipid metabolism in longissimus dorsi of Korean bulls and steers", Meat Sci, 91, pp.284~293 (2012)[DOI]
  5. National Livestock Cooperatives Federation (NLCF), "Korean carcass grading standard", National Livestock Cooperatives Federation Republic of Korea, ~ (1998)
  6. Ribeiro EL, "Growth and carcass characteristics of pasture fed LHRH immunocastrated, castrated and intact Bos indicus bulls", Meat Sci, 68, pp.285~290 (2004)
  7. Xia F, "Castration-induced testosterone deficiency increases fasting glucose associated with hepatic and extra-hepatic insulin resistance in adult male rats", Reprod Biol Endocrinol, 11, pp.106~116 (2013)
  8. Report of business for animal products grading, "Korea institute for Animal Products Quality Evaluation (KAPE)", Korea institute for Animal Products Quality Evaluation, ~ (2013)
  9. Livak KJ, "Analysis of relative gene expression data using real-time quantitative PCR and the <TEX>$2^{-{\Delta}{\Delta}CT}$<", TEX> method, 2001, pp.25, 402~408
  10. Al-Trad B, "Expression and activity of key hepatic gluconeogenesis enzymes in response to increasing intravenous infusions of glucose in dairy cows", J Anim Sci, 88, pp.2998~3008 (2010)[DOI]
  11. Orchard SG, "Substrate specificity of the short chain fatty acyl-coenzyme a synthetase of pinus radiata", Phytochemistry, 41, pp.1465~1472 (1996)[DOI]
  12. Wang LF, "Alteration of factors associated with hepatic gluconeogenesis in response to acute lipopolysaccharide in dairy goat", J Anim Sci, 93, pp.2767~2777 (2015)[DOI]
  13. [URL]
  14. Li WQ, "Effect of two different diets on liver gene expression associated with glucose metabolism in dairy cows", Livest Sci, 158, pp.223~229 (2013)[DOI]
  15. Rui L, "Energy metabolism in the liver", Compr Physiol, 4, pp.177~197 (2014)
  16. Dubois V, "Androgen deficiency exacerbates high-fat diet-induced metabolic alterations in male mice", Endocrinology, 157, pp.648~665 (2016)[DOI]
  17. Hanson RW, "Regulation of phosphoenolpyruvate carboxykinase (GTP) gene expression", Annu Rev Biochem, 66, pp.581~611 (1997)[DOI]
  18. Guo X, "Glycolysis in the control of blood glucose homeostasis", Acta Pharm Sin B, 2, pp.358~367 (2012)[DOI]
  19. Kuhla B, "Involvement of skeletal muscle protein, glycogen, and fat metabolism in the adaptation on early lactation of dairy cows", J Proteome Res, 10, pp.4252~4262 (2011)[DOI]
  20. Edgerton DS, "Effects of insulin on the metabolic control of hepatic gluconeogenesis in vivo", Diabetes, 58, pp.2766~2775 (2009)[DOI]
  21. Lovejoy J, "insulin resistance in obesity is associated with elevated basal lactate levels and diminished lactate appearance following intravenous glucose and insulin", Metabolism, 41, pp.22~27 (1992)
  22. Py G, "Impaired sarcolemmal vesicle lactate uptake and skeletal muscle MCT1 and MCT4 expression in obese Zucker rats", Am J Physiol Endocrinol Metab, 281, pp.E1308~E1315 (2001)[DOI]
  23. Stark R, "The mitochondrial isoform of phosphoenolpyruvate carboxykinase (PEPCK-M) and glucose homeostasis: has it been overlooked?", Biochim Biophys Acta, 1840, pp.1313~1330 (2014)[DOI]
  24. Baik M, "Molecular mechanisms related to bovine intramuscular fat deposition in the longissimus muscle", J Anim Sci, 95, pp.2284~2303 (2017)
  25. Sato T, "Glycerol-3-phosphate dehydrogenase 1 deficiency induces compensatory amino acid metabolism during fasting in mice", Metabolism, 65, pp.1646~1656 (2016)[DOI]
  26. Perry RJ, "Hepatic acetyl CoA links adipose tissue inflammation to hepatic insulin resistance and type 2 diabetes", Cell, 160, pp.745~758 (2015)[DOI]
  27. Adeva-Andany MM, "Glycogen metabolism in humans", BBA Clin, 5, pp.85~100 (2016)[DOI]
  28. Adina-Zada A, "Regulation of the structure and activity of pyruvate carboxylase by acetyl CoA", Arch Biochem Biophys, 519, pp.118~130 (2012)[DOI]
  29. Jeong J, "Transcriptome changes favoring intramuscular fat deposition in the longissimus muscle following castration of bulls", J Anim Sci, 91, pp.4692~4704 (2014)
  30. G.J. Chen, "Effects of Forage:Concentrate Ratio on Growth Performance, Ruminal Fermentation and Blood Metabolites in Housing-feeding Yaks", Animal Bioscience, 28(12), pp.1736~1741 (2015), 아세아·태평양축산학회[DOI]
  31. 정창대, "Rumen fermentation and performance of Hanwoo steers fed total mixed ration with Korean rice wine residue", 한국축산학회지, 58(1), pp.1~7 (2016), 한국축산학회
  32. Brockman RP, "Effect of insulin on the utilization of propionate in gluconeogenesis in sheep", Br J Nutr, 64, pp.95~101 (1990)[DOI]
  33. Michael MD, "Loss of insulin signaling in hepatocytes leads to severe insulin resistance and progressive hepatic dysfunction", Mol Cell, 6, pp.87~97 (2000)[DOI]
  34. Cettour-Rose P, "Redistribution of glucose from skeletal muscle to adipose tissue during catchup fat. a link between catch-up growth and later metabolic syndrome", Diabetes, 54, pp.751~756 (2005)[DOI]
  35. Prior RL, "Effects of intravenous infusions of glucose, lactate, propionate or acetate on the induction of lipogenesis in bovine adipose tissue", J Nutr, 110, pp.2011~2019 (1980)[DOI]
  36. Young OA, "Effect of castration on bovine muscle composition", Meat Sci, 11, pp.139~156 (1984)

관련 논문AI추천

The molecular mechanism of propionate-regulating gluconeogenesis in bovine hepatocytesHuang Li, Li Yu, Mao Tiantian, Pang Rui, Xiao Xiao, Xu Wei, Yu Jiajia, Zhu Wen아세아·태평양축산학회 · Animal Bioscience2023 · 제36권 제11호Effects of Starvation on Lipid Metabolism and Gluconeogenesis in YakJiuqiang Guan, Quanhui Peng, Tianwu An, Xiaolin Luo, Xiaoqiang Yu, Zhisheng Wang아세아·태평양축산학회 · Animal Bioscience2016 · 제29권 제11호Effects of Dietary Restriction on the Expression of Lipid Metabolism and Growth Hormone Signaling Genes in the Longissimus dorsi Muscle of Korean Cattle Steers백명기, H.J. Kang, N.H. Trang아세아·태평양축산학회 · Animal Bioscience2015 · 제28권 제8호Effects of weaning and castration ages on growth performance, blood metabolites, and carcass characteristics in Hanwoo steersByung Ki Park, Eung Gi Kwon1, Gi Hwal Son, Hwan Lim, Il Young Kim, Jae Yoon Shim, Ji Hyung Kim, Jong Suh Shin, Joong Kook Park, Jun Sang Ahn, Min Ji Kim, Sung Myoun Cho한국축산학회 · 한국축산학회지2018 · 제60권 제12호The difference of castration timing of Korean Hanwoo bulls does not significantly affect the carcass characteristics황성구, Delgerzul Baatar, Heeok Hong한국축산학회 · 한국축산학회지2021 · 제63권 제2호Elevated thyroid hormones caused by high concentrate diets participate in hepatic metabolic disorders in dairy cowsCai Liuping, Chen Qu, Mao Shengyong, Ni Yingdong, Wu Chen, Yao Zhihao아세아·태평양축산학회 · Animal Bioscience2022 · 제35권 제8호

이 간행물의 다른 논문 (9건)