Heat-Killed Lactobacillus plantarum KCTC 13314BP Enhances Phagocytic Activity and Immunomodulatory Effects via Activation of MAPK and STAT3 Pathways
2
조회
0
다운로드
6
인용
0
참고문헌
키워드
초록
Identification of novel probiotic strains is of great interest in the field of functional foods. Specific strains of heat-killed bacteria have been reported to exert immunomodulatory effects. Herein, we investigated the immune-stimulatory function of heat-killed Lactobacillus plantarum KCTC 13314BP (LBP). Treatment with LBP significantly increased the production of TNF-α and IL-6 by macrophages. More importantly, LBP was able to enhance the phagocytic activity of macrophages against bacterial particles. Activation of p38, JNK, ERK, NF-κB, and STAT3 was involved in the immunomodulatory function of LBP. LBP treatment significantly increased production of TNF-α by bone marrow-derived macrophages and splenocytes, further confirming the immunostimulatory effect of LBP in primary immune cells. Interestingly, the immunomodulatory effects of LBP were much stronger than those of Lactobacillus rhamnosus GG, a well-known probiotic strain. These results indicate that LBP can be a promising immune-enhancing functional food agent.
Abstract
Identification of novel probiotic strains is of great interest in the field of functional foods. Specific strains of heat-killed bacteria have been reported to exert immunomodulatory effects. Herein, we investigated the immune-stimulatory function of heat-killed Lactobacillus plantarum KCTC 13314BP (LBP). Treatment with LBP significantly increased the production of TNF-α and IL-6 by macrophages. More importantly, LBP was able to enhance the phagocytic activity of macrophages against bacterial particles. Activation of p38, JNK, ERK, NF-κB, and STAT3 was involved in the immunomodulatory function of LBP. LBP treatment significantly increased production of TNF-α by bone marrow-derived macrophages and splenocytes, further confirming the immunostimulatory effect of LBP in primary immune cells. Interestingly, the immunomodulatory effects of LBP were much stronger than those of Lactobacillus rhamnosus GG, a well-known probiotic strain. These results indicate that LBP can be a promising immune-enhancing functional food agent.
참고문헌 (38)
- Laskin DL, "Macrophages and inflammatory mediators in chemical toxicity : a battle of forces", Chem. Res. Toxicol, 22, pp.1376~1385 (2009)
- Gordon S, "Phagocytosis : an immunobiologic process", Immunity, 44, pp.463~475 (2016)
- Wynn TA, "Macrophage biology in development, homeostasis and disease", Nature, 496, pp.445~455 (2013)
- Aderem A, "Mechanisms of phagocytosis in macrophages", Annu. Rev. Immunol, 17, pp.593~623 (1999)
- Lloberas J, "Mitogen-activated protein kinases and mitogen kinase phosphatase 1 : a critical interplay in macrophage biology", Front Mol. Biosci, 3, pp.28~ (2016)
- Rao KM, "MAP kinase activation in macrophages", J. Leukoc. Biol, 69, pp.3~10 (2001)
- Valledor AF, "Selective roles of MAPKs during the macrophage response to IFN-gamma", J. Immunol, 180, pp.4523~4529 (2008)
- Baeuerle PA, "Function and activation of NFkappa B in the immune system", Annu. Rev. Immunol, 12, pp.141~179 (1994)
- Grimm S, "The inducible transcription factor NF-kappa B: structure-function relationship of its protein subunits", Biochem. J, 290(Pt 2), pp.297~308 (1993)
- Ghosh S, "NF-kappa B and Rel proteins : evolutionarily conserved mediators of immune responses", Annu. Rev. Immunol, 16, pp.225~260 (1998)
- Taverniti V, "The immunomodulatory properties of probiotic microorganisms beyond their viability(ghost probiotics : proposal of paraprobiotic concept)", Genes Nutr, 6, pp.261~274 (2011)
- Yavuz Yeşilova, "Effect of Probiotics on the Treatment of Children with Atopic Dermatitis", Annals of Dermatology, 24(2), pp.189~193 (2012), 대한피부과학회[DOI]
- Doege K, "Impact of maternal supplementation with probiotics during pregnancy on atopic eczema in childhood--a meta-analysis", Br. J. Nutr, 107, pp.1~6 (2012)
- Nation ML, "Impact of Lactobacillus reuteri colonization on gut microbiota, inflammation, and crying time in infant colic", Sci. Rep, 7, pp.15047~ (2017)
- Chau K, "Probiotics for infantile colic : a randomized, double-blind, placebo-controlled trial investigating Lactobacillus reuteri DSM 17938", J. Pediatr, 166, pp.74~78 (2015)
- Kadooka Y, "Regulation of abdominal adiposity by probiotics(Lactobacillus gasseri SBT2055)in adults with obese tendencies in a randomized controlled trial", Eur. J. Clin. Nutr, 64, pp.636~643 (2010)
- Andreasen AS, "Effects of Lactobacillus acidophilus NCFM on insulin sensitivity and the systemic inflammatory response in human subjects", Br. J. Nutr, 104, pp.1831~1838 (2010)
- Ahmad K, "Probiotics for the treatment of pediatric helicobacter pylori infection : a randomized double blind clinical trial", Iran J. Pediatr, 23, pp.79~84 (2013)
- Hsieh PS, "Eradication of Helicobacter pylori infection by the probiotic strains Lactobacillus johnsonii MH-68 and L, salivarius ssp. salicinius AP-32", Helicobacter, 17, pp.466~477 (2012)
- Seddik HA, "Lactobacillus plantarum and Its Probiotic and Food Potentialities", Probiotics Antimicrob. Proteins, 9, pp.111~122 (2017)
- Capozzi V, "Biogenic amines degradation b lactobacillus plantarum : toward a potential application in wine", Front Microbiol, 3, pp.122~ (2012)
- Arif IA, "Application of RAPD for molecular characterization of plant species of medicinal value from an arid environment", Genet Mol. Res, 9, pp.2191~2198 (2010)
- Kawashima T, "Lactobacillus plantarum strain YU from fermented foods activates Th1 and protective immune responses", Int. Immunopharmacol, 11, pp.2017~2024 (2011)
- Kikuchi Y, "Oral administration of Lactobacillus plantarum strain AYA enhances IgA secretion and provides survival protection against influenza virus infection in mice", PLoS One, 9, pp.e86416~ (2014)
- Rigaux P, "Immunomodulatory properties of Lactobacillus plantarum and its use as a recombinant vaccine against mite allergy", Allergy, 64, pp.406~414 (2009)
- Rizzo A, "Lactobacillus plantarum reduces Streptococcus pyogenes virulence by modulating the IL-17, IL-23 and Tolllike receptor 2", 4 expressions in human epithelial cells, 2013, pp.17, 453~461
- Segers ME, "Towards a better understanding of Lactobacillus rhamnosus GG--host interactions", Microb. Cell Fact, 13(Suppl 1), pp.S7~ (2014)
- Billack B, "Macrophage activation : role of toll-like receptors, nitric oxide, and nuclear factor kappa B", Am. J. Pharm. Educ, 70, pp.102~ (2006)
- Mosser DM, "Exploring the full spectrum of macrophage activation", Nat. Rev. Immunol, 8, pp.958~969 (2008)
- Fong FL, "Immunomodulation of Lactobacillus rhamnosus GG(LGG)-derived soluble factors on antigen-presenting cells of healthy blood donors", Sci. Rep, 6, pp.22845~ (2016)
- Fong FLY, "Immunomodulatory effects of Lactobacillus rhamnosus GG on dendritic cells, macrophages and monocytes from healthy donors", J. Functional Foods, 13, pp.71~79 (2015)
- Parameswaran N, "Tumor necrosis factoralpha signaling in macrophages", Crit. Rev. Eukaryot Gene Expr, 20, pp.87~103 (2010)
- 이재훈, "In Vitro Immune-Enhancing Activity of Ovotransferrin from Egg White via MAPK Signaling Pathways in RAW 264.7 Macrophages", 한국축산식품학회지, 38(6), pp.1226~1236 (2018), 한국축산식품학회
- Rosales C, "Phagocytosis : a fundamental process in immunity", Biomed. Res. Int, 2017, pp.9042851~ (2017)
- Butprom S, "Effect of Lactobacillus plantarum C014 on innate immune response and disease resistance against Aeromonas hydrophila in hybrid catfish", Scientific World Journal, 2013, pp.392523~ (2013)
- 장세은, "Lactobacillus plantarum HY7712 Ameliorates Cyclophosphamide-Induced Immunosuppression in Mice", Journal of Microbiology and Biotechnology, 23(3), pp.414~421 (2013), 한국미생물·생명공학회[DOI]
- Ren D, "Evaluation of immunomodulatory activity of two potential probiotic Lactobacillus strains by in vivo tests", Anaerobe, 35, pp.22~27 (2015)
- Meng Y, "Immunomodulatory activity of Lactobacillus plantarum KLDS1.0318 in cyclophosphamide-treated mice", Food Nutr. Res, 62, pp.29219~ (2018)[DOI]