The Effect of Human Placental Extract on Rheumatoid Arthritis in an Animal Model
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Objective To assess the effi cacy of human placental extract (HPE) in an animal model of rheumatoid arthritis (RA). Method We used (i) KRN C57BL/6 TCR transgenic x NOD mice (KBx/N) serum transfer arthritis and (ii) collageninduced arthritis (CIA) mice to evaluate the effi cacy of HPE (1 ul or 100 ul, intra-peritoneal, three times per week)on RA. Incidence, severity of arthritis, and hind-paw thickness were quantifi ed. Joint destruction was analyzed using modifi ed mammographic imaging. Histopathological analysis for infl ammation, cartilage, and osteoclasts was performed using Hematoxylin-eosin (H-E), safranin-O, and tartrate-resistant acidic phosphatase (TRAP). ELISAs were used for detection of various cytokines in serum and joint tissue. Results Th ere were no signifi cant diff erences in incidence of arthritis, clinical scores of arthritis, and hind-paw thickness between HPE-treated and vehicle-treated groups for up to 2 weeks in the KBx/N serum transfer arthritis model. Histopathological analysis also showed no diff erences 2 weeks after treatment. Levels of TNF-α, IL-1β, IL-6, IL-10, and RANKL in serum and joint tissues were similar in all groups. Furthermore, there were no diff erences in clinical, radiological, and histological parameters between HPE-treated and vehicle-treated group for 3 weeks in the CIA model. Conclusion Systemic treatment with HPE has no benefi cial eff ects on arthritis in animal models of RA. Th erefore,indiscreet use of HPE in RA should be forbidden.
Abstract
Objective To assess the effi cacy of human placental extract (HPE) in an animal model of rheumatoid arthritis (RA). Method We used (i) KRN C57BL/6 TCR transgenic x NOD mice (KBx/N) serum transfer arthritis and (ii) collageninduced arthritis (CIA) mice to evaluate the effi cacy of HPE (1 ul or 100 ul, intra-peritoneal, three times per week)on RA. Incidence, severity of arthritis, and hind-paw thickness were quantifi ed. Joint destruction was analyzed using modifi ed mammographic imaging. Histopathological analysis for infl ammation, cartilage, and osteoclasts was performed using Hematoxylin-eosin (H-E), safranin-O, and tartrate-resistant acidic phosphatase (TRAP). ELISAs were used for detection of various cytokines in serum and joint tissue. Results Th ere were no signifi cant diff erences in incidence of arthritis, clinical scores of arthritis, and hind-paw thickness between HPE-treated and vehicle-treated groups for up to 2 weeks in the KBx/N serum transfer arthritis model. Histopathological analysis also showed no diff erences 2 weeks after treatment. Levels of TNF-α, IL-1β, IL-6, IL-10, and RANKL in serum and joint tissues were similar in all groups. Furthermore, there were no diff erences in clinical, radiological, and histological parameters between HPE-treated and vehicle-treated group for 3 weeks in the CIA model. Conclusion Systemic treatment with HPE has no benefi cial eff ects on arthritis in animal models of RA. Th erefore,indiscreet use of HPE in RA should be forbidden.
참고문헌 (18)
- Analysis about consumers use of human placental injection and development of public relations technique, "Lee ES", Korea food and drug administration, ~ (2010)
- Sim CU., "The placenta therapy, 1st ed", pp.31~ (2005), MD World
- Park NJ, "Safety, efficacy and limitations of medical use of placental extract", Korean J Med Hist, 48, pp.1013~1021 (2005)
- Firestein GS, "Evolving concepts of rheumatoid arthritis", Nature, 423, pp.356~361 (2003)
- 허준, "태반추출물이 인간 연골세포의 증식과 분화에 미치는 영향", Archives of Plastic Surgery, 33(5), pp.616~620 (2006), 대한성형외과학회
- Kim JK, "Protective effects of human placenta extract on cartilage degradation in experimental osteoarthritis", Biol Pharm Bull, 33, pp.1004~1010 (2010)
- Yeom MJ, "Therapeutic eff ects of Hominis placenta injection into an acupuncture point on the infl ammatory responses in subchondral bone region of adjuvant-induced polyarthritic rat", Biol Pharm Bull, 26, pp.1472~1477 (2003)
- Combe B, "Human placenta-eluted gammaglobulins in immunomodulating treatment of rheumatoid arthritis", Am J Med, 78, pp.920~928 (1985)
- Sany J, "Immunomodulating effect of human placenta-eluted gamma globulins in rheumatoid arthritis", Arthritis Rheum, 25, pp.17~24 (1982)
- Lu J, "Vascular endothelial growth factor expression and regulation of murine collagen-induced arthritis", J Immunol, 164, pp.5922~5927 (2000)
- Mould AW, "Vegfb gene knockout mice display reduced pathology and synovial angiogenesis in both antigen-induced and collagen-induced models of arthritis", Arthritis Rheum, 48, pp.2660~2669 (2003)
- Luttun A, "Revascularization of ischemic tissues by PIGF treatment, and inhibition of tumor angiogenesis, arthritis and atherosclerosis by anti-Flt1", Nat Med, 8, pp.831~840 (2002)
- Yoo SA, "Role of placenta growth factor and its receptor flt-1 in rheumatoid inflammation: a link between angiogenesis and inflammation", Arthritis Rheum, 60, pp.345~354 (2009)
- Kong MH, "Effect of human placental extract on menopausal symptoms, fatigue, and risk factors for cardiovascular disease in middle-aged Korean women", Menopause, 15, pp.296~303 (2008)
- Park YR, "Effect and Safety of Human Placental Extract on Menopausal Symptoms", J Korean Soc Menopause, 15, pp.178~185 (2009)
- Sur TK, "Anti-inflamma tory and anti-platelet aggregation activity of human placental extract", Pharmacol Sin, 24, pp.187~192 (2003)
- Banerjee KK, "Anti-infl amma tory eff ect of human placental extract: a bio che mical mechanistic approach", Riv Eur Sci Med Far macol, 14, pp.361~366 (1992)
- Shibasaki T, "Corticotropin- releasing factor-like activity in human placental extracts", J Clin Endocrinol Metab, 55, pp.384~386 (1982)