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논문KCI 등재

콜라겐 유도 관절염에서 콜라겐 항원 특이 V？3+CD4+ T 세포의 선택적 증식

Selective Expansion of TCR Vβ3+CD4+ T Cells in Collagen-induced Arthritis in DBA/1 Mice

이정은|박성환|김호연|윤종현|민준기|조미라|민소연|김완욱|이재선
대한면역학회 (The Korean Association of Immunologists)|2005.06|Immune Network|제5권 제2호|pp. 78-88

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Abstract

Background: Collagen-induced arthritis (CIA) in mice is animal model of autoimmune disease known as rheumatic arthritis in human. We investigated CII-specific CD4 T cell receptor usage in CIA mice. Methods: In CIA model, draining lymph node (dLN) CD4 T cells and splenocytes at 3rd, 5th, 8th week, we investigated CII-specific T cell proliferation, production of IL-17, IFN-γ, TNF-α, IL-4 and IL-10. And we also performed anti-CII IgG Ab measurements in serum level, TCRVβ usage and T cell clonality with RT-PCR-SSCP analysis. Also, we performed proliferative response against CII when CII-specific T cell subset is deleted. Results: CIA mice showed more increase in the serum level of anti-CII IgG than normal mice after induction of arthritis. And the level of anti-CII IgG2a in CIA mice was increased after 3rd week after primary immunization, while anti-CII IgG1 was decreased. Draining LN CD4 T cells have proliferated against CII stimulation at 3rd week after 1st immunization. CD4 T cells derived from dLN of CIA mice produced proinflammatory cytokine IFN-γ, IL-17 etc. Draining LN CD4 T cells of CIA presented higher proportion of CD4 Vβ3 subsets compared to those of normal mice at 3rd week after 1st immunization, and they were increased in proportion by CII stimulation. Draining LN CD4 T cells without TCRVβ3 /Vβ8.1/8.2 /Vβ10b cells were not responsive against CII stimulation. But, CII-reactive response of TCRVβ3 /Vβ8.1/8.2 /Vβ10b T cells was recovered when Vβ3 T cells were added in culture. Conclusion: Our results indicate that CD4 Vβ3 T cells cells are selectively expanded in dLN of CIA mice, and their recovery upon CII re-stimulation in vitro, as well as the production Th1-type cytokines, may play pivotal role in CIA pathogenesis.

참고문헌 (32)

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