Induction of tolerance against the arthritogenic antigen with type-II collagen peptide-linked soluble MHC class II molecules
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In murine collagen-induced arthritis (CIA), self-reactive T cells can recognize peptide antigens derived from type-II collagen (CII). Activation of T cells is an important mediator of autoimmune diseases. Thus, T cells have become a focal point of study to treat autoimmune diseases. In this study, we evaluated the efficacy of recombinant MHC class II molecules in the regulation of antigen-specific T cells by using a self peptide derived from CII (CII260-274; IAGFKGEQGPKGEPG) linked to mouse I-Aq in a murine CIA model. We found that recombinant I-Aq/CII260-274 molecules could be recognized by CII-specific T cells and inhibit the same T cells in vitro. Furthermore, the development of CIA in mice was successfully prevented by in vivo injection of recombinant I-Aq/CII260-274 molecules. Thus, treatment with recombinant soluble MHC class II molecules in complex with an immunodominant self-peptide might offer a potential therapeutic for chronic inflammation in autoimmune disease such as rheumatoid arthritis.
Abstract
In murine collagen-induced arthritis (CIA), self-reactive T cells can recognize peptide antigens derived from type-II collagen (CII). Activation of T cells is an important mediator of autoimmune diseases. Thus, T cells have become a focal point of study to treat autoimmune diseases. In this study, we evaluated the efficacy of recombinant MHC class II molecules in the regulation of antigen-specific T cells by using a self peptide derived from CII (CII260-274; IAGFKGEQGPKGEPG) linked to mouse I-Aq in a murine CIA model. We found that recombinant I-Aq/CII260-274 molecules could be recognized by CII-specific T cells and inhibit the same T cells in vitro. Furthermore, the development of CIA in mice was successfully prevented by in vivo injection of recombinant I-Aq/CII260-274 molecules. Thus, treatment with recombinant soluble MHC class II molecules in complex with an immunodominant self-peptide might offer a potential therapeutic for chronic inflammation in autoimmune disease such as rheumatoid arthritis.
참고문헌 (31)
- Imboden JB, "The immunopathogenesis of rheumatoid arthritis", Annu Rev Pathol, 4, pp.417~434 (2009)
- Firestein GS, "The T cell cometh: interplay between adaptive immunity, cytokine networks in rheumatoid arthritis", J Clin Invest, 114, pp.471~474 (2004)
- Courtenay JS, "Immunisation against heterologous type II collagen induces arthritis in mice", Nature, 283, pp.666~668 (1980)
- Zanelli E, "Could HLA-DRB1 be the protective locus in rheumatoid arthritis?", Immunol Today, 16, pp.274~278 (1995)
- Trentham DE, "Autoimmunity to type II collagen an experimental model of arthritis", J Exp Med, 146, pp.857~868 (1977)
- Mauri C, "Relationship between Th1", Th2 cytokine patterns, the arthritogenic response in collagen-induced arthritis, 1996, pp.26, 1511~1518
- Ortmann RA, "Susceptibility to collagen-induced arthritis: cytokine-mediated regulation", Clin Immunol, 98, pp.109~118 (2001)
- Miossec P, "Th1", Th2 cytokine balance in arthritis, 1997, pp.40, 2105~2115
- Burrows GG, "Systemic immunomodulation of autoimmune disease using MHC-derived recombinant TCR ligands", Curr Drug Targets Inflamm Allergy, 4, pp.185~193 (2005)
- Quill H, "Stimulation of normal inducer T cell clones with antigen presented by purified Ia molecules in planar lipid membranes: specific induction of a long-lived state of proliferative nonresponsiveness", J Immunol, 138, pp.3704~3712 (1987)
- Schwartz RH, "Models of T cell anergy: is there a common molecular mechanism?", J Exp Med, 184, pp.1~8 (1996)
- Appel H, "Anergy induction by dimeric TCR ligands", J Immunol, 166, pp.5279~5285 (2001)
- Smith-Garvin JE, "T cell activation", Annu Rev Immunol, 27, pp.591~619 (2009)
- DeFord-Watts LM, "The CD3 zeta subunit contains a phosphoinositide-binding motif that is required for the stable accumulation of TCR-CD3 complex at the immunological synapse", J Immunol, 186, pp.6839~6847 (2011)
- Appleman LJ, "T cell anergy, costimulation", Immunol Rev, 192, pp.161~180 (2003)
- Rhode PR, "Single-chain MHC class II molecules induce T cell activation, apoptosis", J Immunol, 157, pp.4885~4891 (1996)
- Nag B, "Soluble MHC II-peptide complexes induce antigen-specific apoptosis in T cells", Cell Immunol, 170, pp.25~33 (1996)
- Hurlin PJ, "Mad3 and Mad4: novel Max-interacting transcriptional repressors that suppress c-myc dependent transformation and are expressed during neural and epidermal differentiation", EMBO J, 15, pp.2030~ (1996)
- Zhang Y, "Synergistic effects of interleukin-1beta, interleukin-17A antibodies on collagen-induced arthritis mouse model", Int Immunopharmacol, 15, pp.199~205 (2013)
- Nakajima A, "Antigen-specific T cell-mediated gene therapy in collagen-induced arthritis", J Clin Invest, 107, pp.1293~1301 (2001)
- Morita Y, "Dendritic cells genetically engineered to express IL-4 inhibit murine collagen-induced arthritis", J Clin Invest, 107, pp.1275~1284 (2001)
- Savage P, "Expansion of CD8+ cytotoxic T cells in vitro, in vivo using MHC class I tetramers", Tumour Biol, 28, pp.70~76 (2007)
- Huan J, "MHC class II derived recombinant T cell receptor ligands protect DBA", 1LacJ mice from collagen-induced arthritis, 2008, pp.180, 1249~1257
- Mealey RH, "Early detection of dominant Env-specific, subdominant Gag-specific CD8+ lymphocytes in equine infectious anemia virus-infected horses using major histocompatibility complex class I", peptide tetrameric complexes, 2005, pp.339, 110~126
- Offner H, "Recombinant T cell receptor ligands: immunomodulatory, neuroprotective, neuroregenerative effects suggest application as therapy for multiple sclerosis", Rev Neurosci, 19, pp.327~339 (2008)
- Huan J, "Monomeric recombinant TCR ligand reduces relapse rate, severity of experimental autoimmune encephalomyelitis in SJL", J mice through cytokine switch, 2004, pp.172, 4556~4566
- Kotzin BL, "Use of soluble peptide-DR4 tetramers to detect synovial T cells specific for cartilage antigens in patients with rheumatoid arthritis", Proc Natl Acad Sci U S A, 97, pp.291~296 (2000)
- Lovitch SB, "Amino-terminal flanking residues determine the conformation of a peptide-class II MHC complex", J Immunol, 176, pp.2958~2968 (2006)
- Ren D, "Construction of bioactive chimeric MHC class I tetramer by expression, purification of human-murine chimeric MHC heavy chain, beta(2)m as a fusion protein in Escherichia coli", Protein Expr Purif, 50, pp.171~178 (2006)
- Brand DD, "Collageninduced arthritis", Nat Protoc, 2, pp.1269~1275 (2007)
- 박주원, "Establishment and characterization of bortezomib-resistant U266 cell line: Constitutive activation of NF-κB-mediated cell signals and", or alterations of ubiquitylation-related genes reduce bortezomib-induced apoptosis, 2014, pp.47(5), 274~279[DOI]
