본문 바로가기
KERD
논문KCI 등재

Protease-activated Receptor 2 is Associated with Activation of Human Macrophage Cell Line THP-1.

이영미|김병수|김대기|문우성|강춘식|태진
대한면역학회 (The Korean Association of Immunologists)|2005.12|Immune Network|제5권 제4호|pp. 195-198

2

조회

0

다운로드

0

인용

0

참고문헌

초록

Abstract

Background: Protease-activated receptor 2 (PAR2) belongs to a family of G protein- coupled receptors activated by proteolytic cleavage. Trypsin-like serine proteases interact with PAR2 expressed by a variety of tissues and immune cells. The aim of our study was to investigate whether PAR2 stimulation can lead to the activation of human macrophages. Methods: PAR2-mediated proliferation of human macrophage cell line THP-1 was measured with MTT assay. We also examined the extracellular regulated kinase (ERK) phosphorylation and cytokine production induced by trypsin and PAR2-agonist using western blot and enzyme-linked immunosorbent assay (ELISA), respectively. Results: Treatment of trypsin or PAR2-activating peptide increased cell proliferation in a dose-dependent manner, and induced the activation of ERK1/2 in THP-1 cells. In addition, trypsin-induced cell proliferation was inhibited by pretreatment of an ERK inhibitor (PD98059) or trypsin inhibitor (SBTI). Moreover, PAR2 activation by trypsin increased the secretion of TNF-α in THP-1 cells. Conclusion: There results suggest that PAR2 activation by trypsin-like serine proteases can induce cell proliferation through the activation of ERK in human macrophage and that PAR2 may play a crucial role in the cell proliferation and cytokine secretion induced by trypsin-like serine proteases.

참고문헌 (26)

  1. "; Mononuclear phagocytes in immune defence", pp.~162 (2002), Mosby
  2. "Protease-activated G protein-coupled receptors on human platelets and endothelial cells", 78, pp.234~241 (1997)
  3. "transducers of proteinase-mediated signaling in inflammation and immune response", 26, pp.1~43 (2005)
  4. "Thrombin signalling and protease-activated receptors", 407, pp.258~264 (2000)
  5. "Molecular cloning of a potential proteinase activated receptor" (1994)
  6. "Agonists of proteinase-activated receptor 2 induce inflammation by a neurogenic mechanism", 6, pp.151~158 (2000)
  7. "Expression of trypsin in vascular endothelial cells FEBS Lett", 409, pp.442~448 (1997)
  8. "a novel agonist of protease-activated receptors 2 and 4" (2004)
  9. "Expression of protease-activated receptor 2 in ulcerative colitis", 9, pp.224~229 (2003)
  10. "The proteinase-activated receptor 2 is induced by inflammatory mediators in human endothelial cells Comparison with the thrombin receptor" (1996)
  11. "Proteinase- activated receptors novel mechanisms of signaling by serine proteases" (1998)
  12. "Proteinase-activated receptor-2-activating peptides induce leukocyte rolling, adhesion, and extravasation in vivo.", 163, pp.5064~5069 (1999)
  13. "Delayed onset of inflammation in protease-activated receptor-2-deficient mice", 165, pp.6504~6510 (2000)
  14. "structural requirements for thrombin receptor activation reveal mechanism of proteolytic unmasking of agonist function" (1992)
  15. "Thrombin-induced interleukin-8 production and its regulation by interferon-γ and prostaglandin E2 in human monocytic U937 cells", 67, pp.223~227 (1999)
  16. "expression by human neutrophils", 110, pp.881~887 (1997)
  17. "Protease-activated receptors 1 and 4 mediate activation of human platelets by thrombin", 103, pp.879~887 (1999)
  18. "Thrombin and trypsin-induced Ca2+ mobilization in human T cell lines through interaction with different protease-activated receptors", 10, pp.309~316 (1996)
  19. "Differential expression and regulation of protease-activated receptors in human peripheral monocytes and monocyte-derived antigen-presenting cells", 102, pp.2645~2652 (2003)
  20. "Protease-activated receptor 3 is a second thrombin receptor in humans", 3, pp.502~506 (1997)
  21. "Protease-activated receptors: sentries for inflammation?.", 21, pp.103~108 (2000)
  22. "Tryptase activates the mitogen-activated protein kinase", activator protein-1 pathway in human peripheral blood eo-sinophils, 2002, pp.169, 2622~2669
  23. "Thrombin-induced interleukin-8 production and its regulation by interferon-γ and prostaglandin E2 in human monocytic U937 cells", 67, pp.223~227 (1999)
  24. "the requirement for and mechanism of spatial and temporal complexity in Ca2+ signals" (2000)
  25. "Trypsin-like protease of mites purification and characterization of trypsin- like protease from mite faecal extract Dermatophagoides farinae relationship between trypsin-like protease and Der f III", i(y), pp.~784 (1993)
  26. "Tryptase from human pulmonary mast cells" (1981)

관련 논문AI추천

Protease-Activated Receptor-2: A Multifaceted Molecular Transducer in the Human SkinHjalte H. Andersen대한피부과학회 · Annals of Dermatology2016 · 제28권 제6호사람별아교세포주에서의 단백분해효소 활성 수용체-2를 매개한 TNF-α 의 분비강옥화, 김미선, 김진아, 김형민, 백옥선, 엄재영, 윤기중, 이영미, 이진무대한병리학회 · Journal of Pathology and Translational Medicine2003 · 제37권 제3호Thrombin inhibits HMGB1-mediated proinflammatory signaling responses when endothelial protein C receptor is occupied by its natural ligand배종섭, Alireza R. Rezaie생화학분자생물학회 · BMB Reports2013 · 제46권 제11호Inhibition of Lipopolysaccharide-stimulated Inflammatory Cytokine Production by LY303511 in Human Macrophagic THP-1 Cells강인철, 김소희, 백윤웅대한구강생물학회 · International Journal of Oral Biology2017 · 제42권 제3호Deciphering Macrophage Phenotypes upon Lipid Uptake and Atherosclerosis이지혜, 최재훈대한면역학회 · Immune Network2020 · 제20권 제3호Regulation of Macrophage Activation and Differentiation in Atherosclerosis박성호한국지질동맥경화학회 · 지질·동맥경화학회지2021 · 제10권 제3호

이 간행물의 다른 논문 (8건)

Effect of Nitric Oxide on ADP-ribose Pyrophosphatase Activity김종현pp. 199-204Ulcerative Colitis is Associated with Novel Polymorphisms in the Promoter Region of MIP-3α/CCL20 Gene이성가, 서점성, 이명수, 이은경, 김상욱, 채수진, 전창덕, 염주진, 최석제pp. 205-214마우스 내장 림프조직에서 우세하게 발현되는 IgA isotype switching 관련 전사체의 분석서구영, 김현아, 전성기, 김평현, 채병철pp. 215-220제2형 콜라겐 경구관용 유도 동물모델에서 수지상 세포의 Indoleamine 2,3-dioxygenase 의존성 관절염 항원 특이 T세포 증식반응 제어 연구박경수, 조미라, 조영규, 민소연, 박민정, 박성환, 김호연, 윤종현, 민준기pp. 221-231훈련방법의 차이가 SOD, Neutrophils 및 T세포에 미치는 영향김동은, 황혜진, 곽이섭, 엄상용pp. 232-236
원문 보기