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Vitamin C Up-regulates Expression of CD80, CD86 and MHC Class II on Dendritic Cell Line, DC-1 Via the Activation of p38 MAPK

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Vitamin C is an essential water-soluble nutrient which primarily exerts its effect on host defense mechanisms and immune homeostasis, but the mechanism related to immune- potentiation is poorly understood. Since dendritic cells (DCs) are known as a potent antigen presenting cell (APC) that could enhance the antigen specific immune responses, we investigate the effects of vitamin C on activation of DCs and its related mechanism by using dendritic cell lines, DC-1. First, we found that there was no damage on DC-1 by 2.5 mM of vitamin C. In the presence of vitamin C, the expression of CD80,CD86, and MHC molecules was increased, but it was decreased by the pre-treatment of SB203580, p38 MAPK-specific inhibitor. We confirmed the phosphorylation of p38MAPK was increased by the treatment of vitamin C. Taken together, these results suggest that vitamin C could enhance the activity of dendritic cells via the up-regulation of the expression of CD80, CD86, and MHC molecules and the activation of p38 MAPK is related to this process.

Abstract

Vitamin C is an essential water-soluble nutrient which primarily exerts its effect on host defense mechanisms and immune homeostasis, but the mechanism related to immune- potentiation is poorly understood. Since dendritic cells (DCs) are known as a potent antigen presenting cell (APC) that could enhance the antigen specific immune responses, we investigate the effects of vitamin C on activation of DCs and its related mechanism by using dendritic cell lines, DC-1. First, we found that there was no damage on DC-1 by 2.5 mM of vitamin C. In the presence of vitamin C, the expression of CD80,CD86, and MHC molecules was increased, but it was decreased by the pre-treatment of SB203580, p38 MAPK-specific inhibitor. We confirmed the phosphorylation of p38MAPK was increased by the treatment of vitamin C. Taken together, these results suggest that vitamin C could enhance the activity of dendritic cells via the up-regulation of the expression of CD80, CD86, and MHC molecules and the activation of p38 MAPK is related to this process.

참고문헌 (37)

  1. Härtel, C., "Effects of vitamin C on intracytoplasmic cytokine production in human whole blood monocytes and lymphocytes", Cytokine, 27, pp.101~106 (2004)
  2. Hartel, C, "Immunomodulatory effect of vitamin C on intracytoplasmic cytokine production in neonatal cord blood cells", Neonatology, 91, pp.54~60 (2007)
  3. Bergsten, P., "Millimolar concentrations of ascorbic acid in purified human mononuclear leukocytes. Depletion and reaccumulation", J. Biol. Chem, 265, pp.2584~2587 (1990)
  4. May, J. M, "Macrophage uptake and recycling of ascorbic acid: response to activation by lipopolysaccharide", Free Radic. Biol. Med, 39, pp.1449~1459 (2005)
  5. Washko, P., "Ascorbic acid in human neutrophils", Am. J. Clin. Nutr, 54(6), pp.1221S~1227S (1991)
  6. Levine, M., "Vitamin C pharmacokinetics in healthy volunteers: evidence for a recommended dietary allowance", Proc. Natl. Acad. Sci. U.S.A, 93, pp.3704~3709 (1996)
  7. Anderson, R., "The effects of increasing weekly doses of ascorbate on certain cellular and humoral immune functions in normal volunteers", Am. J. Clin. Nutr, 33, pp.71~76 (1980)
  8. Anderson, R., "Vitamin C and cellular immune functions. Protectionagainst hypochlorous acid-mediated inactivation of glyceraldehyde-3-phosphate dehydrogenase and ATP generation inhuman leukocytes as a possible mechanism of ascorbatemediatedimmunostimulation", Ann. N.Y. Acad. Sci, 587, pp.34~48 (1990)
  9. Levy, R, "Vitamin C for the treatment of recurrent furunculosis in patients with imparied neutrophil functions", J. Infect. Dis, 173, pp.1502~1505 (1996)
  10. Jacob, R. A, "Immunocompetence and oxidant defense during ascorbate depletion of healthy men", Am. J. Clin. Nutr, 54(6), pp.1302S~1309S (1991)
  11. Heuser, G, "Enhancement of natural killer cell activity and T and B cell function by buffered vitamin C in patients exposed to toxic chemicals: the role of protein kinase-C", Immunopharmacol. Immunotoxicol, 19, pp.291~312 (1997)
  12. Noh, K., "Mega-dose Vitamin C modulates T cell functions in Balb", c mice only when administered during T cell activation, 2005, pp.98, 63~72
  13. Kay, N. E., "Human T-cell function in experimental ascorbic acid deficiency and spontaneous scurvy", Am. J. Clin. Nutr, 36, pp.127~130 (1982)
  14. Lee, C. W, "Vitamin C supplementation does not protect L-gulono- gamma-lactone oxidase-deficient mice from Helicobacter pylori- induced gastritis and gastric premalignancy", Int. J. Cancer, 122, pp.1068~1076 (2008)
  15. Banchereau, J, "Immunobiology of dendritic cells", Annu. Rev. Immunol, 18, pp.767~811 (2000)
  16. Kumaraguru, U., "Toll-like receptor ligand links innate and adaptive immune responses by the production of heat-shock proteins", J. Leukoc. Biol, 73, pp.574~583 (2003)
  17. Asai, Y., "Toll-like receptor2-mediated dendritic cell activation by a Porphyromonas gingivalissynthetic lipopeptide", J. Med. Microbiol, 56, pp.459~465 (2007)
  18. Gaddis, D. E., "Requirement of TLR4 and CD14 in dendritic cell activation by Hemagglutinin B from Porphyromonas gingivalis", Mol. Immunol, 46, pp.2493~2504 (2009)
  19. Sinha, A., "Impaired generation of reactive oxygen species duringdifferentiation of dendritic cells (DCs) by Mycobacterium tuberculosissecretory antigen (MTSA) and subsequent activationof MTSA-DCs by mycobacteria results in increased intracellularsurvival", J. Immunol, 177, pp.468~478 (2006)
  20. Wang, D., "Elevated mitochondrial reactive oxygen species generation affects the immune response via hypoxia-inducible factor-1alpha in long-lived Mclk1+", − mouse mutants, 2010, pp.184, 582~590
  21. 김영석, "Vitamin C downregulates interleukin-18 production by increasing reactive oxygen intermediate and mitogen-activated protein kinase signalling in B16F10 murine melanoma cells", MELANOMA RESEARCH, 13, pp.549~554, LIPPINCOTT WILLIAMS & WILKINS
  22. Levine, M., "Vitamin C pharmacokinetics in healthy volunteers: evidence for a recommended dietary allowance", Proc. Natl. Acad. Sci. U.S.A, 93, pp.3704~3709 (1996)
  23. Levine, M., "A new recommended dietary allowance of vitamin C for healthy young women", Proc. Natl. Acad. Sci. U.S.A, 98, pp.9842~9846 (2001)
  24. Padayatty, S. J, "Vitamin C pharmacokinetics: implications for oral and intravenous use", Ann. Intern. Med, 140, pp.533~537 (2004)
  25. Kang, J. S, "L-ascorbic acid (vitamin C) induces the apoptosis of B16 murine melanoma cells via a caspase-8-independent pathway. Cancer Immunol", Immunother, 52, pp.693~698 (2003)
  26. Kang, J. S, "Sodium ascorbate (vitamin C) induces apoptosis in melanoma cells via the down-regulation of transferrin receptor dependent iron uptake", J. Cell. Physiol, 204, pp.192~197 (2005)
  27. Hahm, E., "The molecular mechanisms of vitamin C on cell cycle regulation in B16F10 murine melanoma", J. Cell. Biochem, 102, pp.1002~1010 (2007)
  28. Lee, S. K., "Vitamin C suppresses proliferation of the human melanoma cell SK-MEL-2 through the inhibition of cyclooxygenase-2 (COX-2) expression and the modulation of insulin-like growth factor II (IGF-II) production", J. Cell. Physiol, 216, pp.180~188 (2008)
  29. 김하나, "Vitamin C down-regulates VEGF production in B16F10 murine melanoma cells via the suppression of p42", 44 MAPK activation, 201103, pp.112(3), 894~901[DOI]
  30. 유연실, "The Anti-tumor Activity of Vitamin C via the Increase of Fas (CD95) and MHC I expression on Human Stomach Cancer Cell Line, SNU1", Immune Network, 11(4), pp.210~215 (2011), 대한면역학회
  31. Iijima, N., "Selective regulation of CD40 expression in murine dendritic cells by thiol antioxidants", Immunology, 110, pp.197~205 (2003)
  32. Tomiyama, C., "Suppressive role of hepatic dendritic cells in concanavalin A-induced hepatitis", Clin. Exp. Immunol, 166, pp.258~268 (2011)
  33. Chen, K. D., "Adaptor protein Shc acts as an immune-regulator for the LPS-stimulated maturation of bone marrow-derived dendritic cells", BMC Immunol, 12, pp.32~ (2011)
  34. Linster, C. L, "Vitamin C. Biosynthesis, recycling and degradation in mammals", FEBS J, 274, pp.1~22 (2007)
  35. Tsukaguchi, H., "A family of mammalian Na+-dependent L-ascorbic acid transporters", Nature, 399, pp.70~75 (1999)
  36. Daruwala, R, "Cloning and functional characterization of the human sodium-dependent vitamin C transporters hSVCT1 and hSVCT2", FEBS Lett, 460, pp.480~484 (1999)
  37. 김혜민, "The Analysis of Vitamin C Concentration in Organs of Gulo−", − Mice Upon Vitamin C Withdrawal, 2012, pp.12(1), 18~26[DOI]

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