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감송향이 수지상세포 성숙에 미치는 영향

Inhibitory effects of Nardostachys Jatamansi on the maturation of dendritic cells

최창민|정현철|정지혜|김송백|조한백|오광우
대한한방부인과학회 (The Society Of Oriental Obstetrics & Gynecology)|2010.08|대한한방부인과학회지 (The Journal of Oriental Obstetrics ＆ Gynecology)|제23권 제3호|pp. 14-25

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참고문헌

키워드

Nardostachys Jatamansi(NJ)Dendritic cellsMaturation.
주제분류한의학

초록

Purpose: The purpose of this study is to investigate inhibitory effect on the maturation of dendritic cells from aqueous extract from Nardostachys Jatamansi(NJ). Methods: I examined the phenotypic maturation(class Ⅱ MHC, CD40, CD86), expression of pro-inflammatory cytokine(TNF-α, IL-6, IL-12) and endocytosis of FITC-Dextran in the LPS-induced bone marrow-derived dendritic cells(BMDCs) of mice. Furthermore, the Western-blot analysis reveals the mechanism of inhibitory effect. Results:1. The NJ extract inhibited the phenotypic maturation of BMDCs in a dose- dependent manner. 2. The NJ extract inhibited the LPS induced cytokine production of BMDCs in a dose-dependent manner. 3. The NJ extract enhanced the endocytosis of Dex-FITC in LPS treated DC. 4. The NJ extract inhibited the activation of JNK and p38 phosphorylation, but not ERK phosphorylation of MAPK family and doesn't inhibit Ik-Ba degradation in LPS-stimulated BMDCs. Conclusion: These results suggest that NJ extract is able to attenuate the inflammation and maturation in BMDCs and may inhibit proliferation of T cells. In conclusion, this experiment suggests that NJ extract may be useful in hypersensitivity disease including autoimmune disease.

Abstract

Purpose: The purpose of this study is to investigate inhibitory effect on the maturation of dendritic cells from aqueous extract from Nardostachys Jatamansi(NJ). Methods: I examined the phenotypic maturation(class Ⅱ MHC, CD40, CD86), expression of pro-inflammatory cytokine(TNF-α, IL-6, IL-12) and endocytosis of FITC-Dextran in the LPS-induced bone marrow-derived dendritic cells(BMDCs) of mice. Furthermore, the Western-blot analysis reveals the mechanism of inhibitory effect. Results:1. The NJ extract inhibited the phenotypic maturation of BMDCs in a dose- dependent manner. 2. The NJ extract inhibited the LPS induced cytokine production of BMDCs in a dose-dependent manner. 3. The NJ extract enhanced the endocytosis of Dex-FITC in LPS treated DC. 4. The NJ extract inhibited the activation of JNK and p38 phosphorylation, but not ERK phosphorylation of MAPK family and doesn't inhibit Ik-Ba degradation in LPS-stimulated BMDCs. Conclusion: These results suggest that NJ extract is able to attenuate the inflammation and maturation in BMDCs and may inhibit proliferation of T cells. In conclusion, this experiment suggests that NJ extract may be useful in hypersensitivity disease including autoimmune disease.

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