Effect of resveratrol on the metastasis of 4T1 mouse breast cancer cells in vitro and in vivo
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We investigated the effects of resveratrol on metastasis in in vitro and in vivo systems. 4T1 cells were cultured in the presence of various concentrations (0-30 μmol/L) of resveratrol. For experimental metastasis, BALB/c mice were injected intravenously with 4T1 cells in the tail vein, and were orally administered various concentrations (0, 100, or 200 mg/kg Body weight) of resveratrol for 21 days. After resveratrol treatment, cell adhesion, wound migration, invasion, and MMP-9 activity were significantly decreased in a dose-dependent manner in 4T1 cells (P < 0.05). The numbers of pulmonary nodules were significantly decreased in mice fed the resveratrol (P < 0.05). The plasma MMP-9 activity was decreased in response to treatment with resveratrol in mice (P < 0.05). We conclude that resveratrol inhibits cancer metastasis both in vitro and in vivo, and this inhibition is likely due to the decrease in MMP-9 activity caused by resveratrol.
Abstract
We investigated the effects of resveratrol on metastasis in in vitro and in vivo systems. 4T1 cells were cultured in the presence of various concentrations (0-30 μmol/L) of resveratrol. For experimental metastasis, BALB/c mice were injected intravenously with 4T1 cells in the tail vein, and were orally administered various concentrations (0, 100, or 200 mg/kg Body weight) of resveratrol for 21 days. After resveratrol treatment, cell adhesion, wound migration, invasion, and MMP-9 activity were significantly decreased in a dose-dependent manner in 4T1 cells (P < 0.05). The numbers of pulmonary nodules were significantly decreased in mice fed the resveratrol (P < 0.05). The plasma MMP-9 activity was decreased in response to treatment with resveratrol in mice (P < 0.05). We conclude that resveratrol inhibits cancer metastasis both in vitro and in vivo, and this inhibition is likely due to the decrease in MMP-9 activity caused by resveratrol.
참고문헌 (47)
- Zhou HB, "Resveratrol induces apoptosis in human esophageal carcinoma cells", World J Gastroenterol, 9, pp.408~411 (2003)
- Kopp P., "Resveratrol, a phytoestrogen found in red wine. A possible explanation for the conundrum of the 'French paradox'?", Eur J Endocrinol, 138, pp.619~620 (1998)
- Jang M, "Cancer chemopreventive activity of resveratrol", Drugs Exp Clin Res, 25, pp.65~77 (1999)
- Jang M, "Cancer chemopreventive activity of resveratrol, a natural product derived from grapes", Science, 275, pp.218~220 (1997)
- Frankel EN, "Inhibition of human LDL oxidation by resveratrol", Lancet, 341, pp.1103~1104 (1993)
- Fauconneau B, "Comparative study of radical scavenger and antioxidant properties of phenolic compounds from Vitis vinifera cell cultures using in vitro tests", Life Sci, 61, pp.2103~2110 (1997)
- Pace-Asciak CR, "Wines and grape juices as modulators of platelet aggregation in healthy human subjects", Clin Chim Acta, 246, pp.163~182 (1996)
- Liu BL, "New enlightenment of French Paradox: resveratrol's potential for cancer chemoprevention and anti-cancer therapy", Cancer Biol Ther, 6, pp.1833~1836 (2007)
- Sun AY, "The "French Paradox" and beyond: neuroprotective effects of polyphenols", Free Radic Biol Med, 32, pp.314~318 (2002)
- Aggarwal BB, "Role of resveratrol in prevention and therapy of cancer: preclinical and clinical studies", Anticancer Res, 24, pp.2783~2840 (2004)
- Athar M, "Resveratrol: a review of preclinical studies for human cancer prevention", Toxicol Appl Pharmacol, 224, pp.274~283 (2007)
- Yoon SO, "Inhibitory effect of selenite on invasion of HT1080 tumor cells", J Biol Chem, 276, pp.20085~20092 (2001)
- Mook OR, "The role of gelatinases in colorectal cancer progression and metastasis", Biochim Biophys Acta, 1705, pp.69~89 (2004)
- Srivastava KC, "Aqueous extracts of onion, garlic and ginger inhibit platelet aggregation and alter arachidonic acid metabolism", Biomed Biochim Acta, 43, pp.335~346 (1984)
- Freije JM, "Matrix metalloproteinases and tumor progression", Adv Exp Med Biol, 532, pp.91~107 (2003)
- Ja Eun Kim, "LYR71, a derivative of trimeric resveratrol, inhibits tumorigenesis by blocking STAT3-mediated matrix metalloproteinase 9 expression", Experimental and Molecular Medicine, 40(5), pp.514~522 (2008), 생화학분자생물학회
- Tao K, "Imagable 4T1 model for the study of late stage breast cancer", BMC Cancer, 8, pp.228~ (2008)
- -Gingerol inhibits metastasis of MDA-MB-231 human breast cancer cells, "JOURNAL OF NUTRITIONAL BIOCHEMISTRY", 200805, 313~319, pp.ELSEVIER SCIENCE INC
- Soel SM, "Influence of conjugated linoleic acid isomers on the metastasis of colon cancer cells in vitro and in vivo", J Nutr Biochem, 18, pp.650~657 (2007)
- Reeves PG, "AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet", J Nutr, 123, pp.1939~1951 (1993)
- Pignatelli M, "Integrins in tumour development and spread", Cancer Surv, 24, pp.113~127 (1995)
- Stetler-Stevenson WG, "Tumor cell interactions with the extracellular matrix during invasion and metastasis", Annu Rev Cell Biol, 9, pp.541~573 (1993)
- Yu AE, "Molecular regulation of cellular invasion--role of gelatinase A and TIMP-2", Biochem Cell Biol, 74, pp.823~831 (1996)
- Liotta LA, "Metastatic potential correlates with enzymatic degradation of basement membrane collagen", Nature, 284, pp.67~68 (1980)
- Chen J, "Lignans and tamoxifen, alone or in combination, reduce human breast cancer cell adhesion, invasion and migration in vitro", Breast Cancer Res Treat, 80, pp.163~170 (2003)
- Sternlicht MD, "How matrix metalloproteinases regulate cell behavior", Annu Rev Cell Dev Biol, 17, pp.463~516 (2001)
- Egeblad M, "New functions for the matrix metalloproteinases in cancer progression", Nat Rev Cancer, 2, pp.161~174 (2002)
- Scorilas A, "Overexpression of matrix- metalloproteinase- 9 in human breast cancer: a potential favourable indicator in node-negative patients", Br J Cancer, 84, pp.1488~1496 (2001)
- Tang FY, "Resveratrol inhibits heregulinbeta1- mediated matrix metalloproteinase-9 expression and cell invasion in human breast cancer cells", J Nutr Biochem, 19, pp.287~294 (2008)
- Pan MH, "Pterostilbene inhibited tumor invasion via suppressing multiple signal transduction pathways in human hepatocellular carcinoma cells", Carcinogenesis, 30, pp.1234~1242 (2009)
- Banerjee S, "Suppression of 7,12- dimethylbenz(a)anthracene-induced mammary carcinogenesis in rats by resveratrol: role of nuclear factor-kappaB, cyclooxygenase 2, and matrix metalloprotease 9", Cancer Res, 62, pp.4945~4954 (2002)
- Yu H, "Crosstalk between cancer and immune cells: role of STAT3 in the tumour microenvironment", Nat Rev Immunol, 7, pp.41~51 (2007)
- Manna SK, "Resveratrol suppresses TNF-induced activation of nuclear transcription factors NF-kappa B, activator protein-1, and apoptosis: potential role of reactive oxygen intermediates and lipid peroxidation", J Immunol, 164, pp.6509~6519 (2000)
- Birrell MA, "Resveratrol, an extract of red wine, inhibits lipopolysaccharide induced airway neutrophilia and inflammatory mediators through an NF-kappaB-independent mechanism", FASEB J, 19, pp.840~841 (2005)
- Kowalski J, "Effect of apigenin, kaempferol and resveratrol on the expression of interleukin-1beta and tumor necrosis factor-alpha genes in J774.2 macrophages", Pharmacol Rep, 57, pp.390~394 (2005)
- Bove K, "Effect of resveratrol on growth of 4T1 breast cancer cells in vitro and in vivo", Biochem Biophys Res Commun, 291, pp.1001~1005 (2002)
- Carbó N, "Resveratrol, a natural product present in wine, decreases tumour growth in a rat tumour model", Biochem Biophys Res Commun, 254, pp.739~743 (1999)
- Kimura Y, "Resveratrol isolated from Polygonum cuspidatum root prevents tumor growth and metastasis to lung and tumor-induced neovascularization in Lewis lung carcinomabearing mice", J Nutr, 131, pp.1844~1849 (2001)
- Weng YL, "Oral administration of resveratrol in suppression of pulmonary metastasis of BALB", c mice challenged with CT26 colorectal adenocarcinoma cells, 2010, pp.54, 259~267
- Busquets S, "Resveratrol, a natural diphenol, reduces metastatic growth in an experimental cancer model", Cancer Lett, 245, pp.144~148 (2007)
- Bhat KP, "Estrogenic and antiestrogenic properties of resveratrol in mammary tumor models", Cancer Res, 61, pp.7456~7463 (2001)
- Li D, "Resveratrol inhibits MMP-2 expression of hepatoma in nude mice", J Anim Vet Adv, 10, pp.33~37 (2011)
- Chen Y, "Resveratrol-induced cellular apoptosis and cell cycle arrest in neuroblastoma cells and antitumor effects on neuroblastoma in mice", Surgery, 136, pp.57~66 (2004)
- Tseng SH, "Resveratrol suppresses the angiogenesis and tumor growth of gliomas in rats", Clin Cancer Res, 10, pp.2190~2202 (2004)
- Harper CE, "Resveratrol suppresses prostate cancer progression in transgenic mice", Carcinogenesis, 28, pp.1946~1953 (2007)
- Weng CJ, "Mechanisms of apoptotic effects induced by resveratrol, dibenzoylmethane, and their analogues on human lung carcinoma cells", J Agric Food Chem, 57, pp.5235~5243 (2009)
- Liu PL, "Resveratrol inhibits human lung adenocarcinoma cell metastasis by suppressing heme oxygenase 1-mediated nuclear factorkappaB pathway and subsequently downregulating expression of matrix metalloproteinases", Mol Nutr Food Res, 54(Suppl 2), pp.196~204 (2010)
