Inhibitory effect of Erythronium japonicum on the human breast cancer cell metastasis
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BACKGROUND/OBJECTIVES: In this study, the inhibitory effect of Erythronium japonicum extracts on the metastasis of MDA-MB-231human breast cancer cell line was determined. MATERIALS/METHODS: Cells were cultured with DMSO or with 50, 75, 100 or 250 μg/ml of Erythronium japonicum methanolor ethanol extract. RESULTS: Both methanol and ethanol extracts significantly inhibited the growth and induced apoptosis of MDA-MB-231 cellsin a dose-dependent manner. Erythronium japonicum extracts inhibited the adhesion of MDA-MB-231 cells. The invasion ofbreast cancer cells was suppressed by Erythronium japonicum extracts in a dose-dependent manner. The motility and MMP-2and MMP-9 activities were also inhibited by both methanol and ethanol extracts. CONCLUSIONS: Our results collectively indicate that Erythronium japonicum extracts inhibit the growth, adhesion, migrationand invasion as well as induce the apoptosis of human breast cancer cells. Clinical application of Erythronium japonicum asa potent chemopreventive agent may be helpful in limiting breast cancer invasion and metastasis.
Abstract
BACKGROUND/OBJECTIVES: In this study, the inhibitory effect of Erythronium japonicum extracts on the metastasis of MDA-MB-231human breast cancer cell line was determined. MATERIALS/METHODS: Cells were cultured with DMSO or with 50, 75, 100 or 250 μg/ml of Erythronium japonicum methanolor ethanol extract. RESULTS: Both methanol and ethanol extracts significantly inhibited the growth and induced apoptosis of MDA-MB-231 cellsin a dose-dependent manner. Erythronium japonicum extracts inhibited the adhesion of MDA-MB-231 cells. The invasion ofbreast cancer cells was suppressed by Erythronium japonicum extracts in a dose-dependent manner. The motility and MMP-2and MMP-9 activities were also inhibited by both methanol and ethanol extracts. CONCLUSIONS: Our results collectively indicate that Erythronium japonicum extracts inhibit the growth, adhesion, migrationand invasion as well as induce the apoptosis of human breast cancer cells. Clinical application of Erythronium japonicum asa potent chemopreventive agent may be helpful in limiting breast cancer invasion and metastasis.
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