Cordyceps militaris alleviates non-alcoholic fatty liver disease in ob/ob mice
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BACKGROUND/OBJECTIVES: Non-alcoholic fatty liver disease (NAFLD) is becoming an important public health problem as metabolicsyndrome and type 2 diabetes have become epidemic. In this study we investigated the protective effect of Cordyceps militaris(C. militaris) against NAFLD in an obese mouse model. MATERIALS/METHODS: Four-week-old male ob/ob mice were fed an AIN-93G diet or a diet containing 1% C. militaris waterextract for 10 weeks after 1 week of adaptation. Serum glucose, insulin, free fatty acid (FFA), alanine transaminase (ALT), andproinflammatory cytokines were measured. Hepatic levels of lipids, glutathione (GSH), and lipid peroxide were determined. RESULTS: Consumption of C. militaris significantly decreased serum glucose, as well as homeostasis model assessment for insulinresistance (HOMA-IR), in ob/ob mice. In addition to lowering serum FFA levels, C. militaris also significantly decreased hepatictotal lipids and triglyceride contents. Serum ALT activities and tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) levelswere reduced by C. militaris. Consumption of C. militaris increased hepatic GSH and reduced lipid peroxide levels. CONCLUSIONS: These results indicate that C. militaris can exert protective effects against development of NAFLD, partly byreducing inflammatory cytokines and improving hepatic antioxidant status in ob/ob mice.
Abstract
BACKGROUND/OBJECTIVES: Non-alcoholic fatty liver disease (NAFLD) is becoming an important public health problem as metabolicsyndrome and type 2 diabetes have become epidemic. In this study we investigated the protective effect of Cordyceps militaris(C. militaris) against NAFLD in an obese mouse model. MATERIALS/METHODS: Four-week-old male ob/ob mice were fed an AIN-93G diet or a diet containing 1% C. militaris waterextract for 10 weeks after 1 week of adaptation. Serum glucose, insulin, free fatty acid (FFA), alanine transaminase (ALT), andproinflammatory cytokines were measured. Hepatic levels of lipids, glutathione (GSH), and lipid peroxide were determined. RESULTS: Consumption of C. militaris significantly decreased serum glucose, as well as homeostasis model assessment for insulinresistance (HOMA-IR), in ob/ob mice. In addition to lowering serum FFA levels, C. militaris also significantly decreased hepatictotal lipids and triglyceride contents. Serum ALT activities and tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) levelswere reduced by C. militaris. Consumption of C. militaris increased hepatic GSH and reduced lipid peroxide levels. CONCLUSIONS: These results indicate that C. militaris can exert protective effects against development of NAFLD, partly byreducing inflammatory cytokines and improving hepatic antioxidant status in ob/ob mice.
참고문헌 (43)
- Sanyal AJ, "AGA technical review on nonalcoholic fatty liver disease", Gastroenterology, 123, pp.1705~1725 (2002)
- Petta S, "Non-alcoholic fatty liver disease pathogenesis: the present and the future", Dig Liver Dis, 41, pp.615~625 (2009)
- Day CP, "Steatohepatitis: a tale of two "hits"?", Gastroenterology, 114, pp.842~845 (1998)
- Salt WB 2nd, "Nonalcoholic fatty liver disease (NAFLD): a comprehensive review", J Insur Med, 36, pp.27~41 (2004)
- Ahmed MH, "Non-Alcoholic Fatty Liver Disease (NAFLD): new challenge for general practitioners and important burden for health authorities?", Prim Care Diabetes, 4, pp.129~137 (2010)
- Day CP, "From fat to inflammation", Gastroenterology, 130, pp.207~210 (2006)
- Marchesini G, "NASH: from liver diseases to metabolic disorders and back to clinical hepatology", Hepatology, 35, pp.497~499 (2002)
- Tilg H, "Insulin resistance, inflammation, and nonalcoholic fatty liver disease", Trends Endocrinol Metab, 19, pp.371~379 (2008)
- Lebovics E, "Non-alcoholic fatty liver disease (NAFLD): why you should care, when you should worry, what you should do", Diabetes Metab Res Rev, 27, pp.419~424 (2011)
- Angulo P, "Obesity and nonalcoholic fatty liver disease", Nutr Rev, 65, pp.S57~S63 (2007)
- Ibrahim MA, "Nonalcoholic fatty liver disease: current and potential therapies", Life Sci, 92, pp.114~118 (2013)
- Angulo P, "Nonalcoholic fatty liver disease", N Engl J Med, 346, pp.1221~1231 (2002)
- Paterson RR, "Cordyceps: a traditional Chinese medicine and another fungal therapeutic biofactory?", Phytochemistry, 69, pp.1469~1495 (2008)
- Ng TB, "Pharmacological actions of Cordyceps, a prized folk medicine", J Pharm Pharmacol, 57, pp.1509~1519 (2005)
- Yu HM, "Comparison of protective effects between cultured Cordyceps militaris and natural Cordyceps sinensis against oxidative damage", J Agric Food Chem, 54, pp.3132~3138 (2006)
- Zhan Y, "Antioxidant activities of aqueous extract from cultivated fruit-bodies of Cordyceps militaris (L.) Link in vitro", J Integr Plant Biol, 48, pp.1365~1370 (2006)
- Wang BS, "Comparison of the hepatoprotective activity between cultured Cordyceps militaris and natural Cordyceps sinensis", J Funct Foods, 4, pp.489~495 (2012)
- 조월순, "The Anti-inflammatory Effects of Water Extract from Cordyceps militaris in Murine Macrophage", Mycobiology, 38(1), pp.46~51 (2010), 한국균학회
- Anstee QM, "Mouse models in non-alcoholic fatty liver disease and steatohepatitis research", Int J Exp Pathol, 87, pp.1~16 (2006)
- Weisberg SP, "Dietary curcumin significantly improves obesity-associated inflammation and diabetes in mouse models of diabesity", Endocrinology, 149, pp.3549~3558 (2008)
- Bruno RS, "Green tea extract protects leptin-deficient, spontaneously obese mice from hepatic steatosis and injury", J Nutr, 138, pp.323~331 (2008)
- Massart J, "Pentoxifylline aggravates fatty liver in obese and diabetic ob", ob mice by increasing intestinal glucose absorption and activating hepatic lipogenesis, 2012, pp.165, 1361~1374
- Zhang G, "Hypoglycemic activity of the fungi Cordyceps militaris, Cordyceps sinensis, Tricholoma mongolicum, and Omphalia lapidescens in streptozotocin-induced diabetic rats", Appl Microbiol Biotechnol, 72, pp.1152~1156 (2006)
- 최하늘, "Effect of Dongchunghacho (Cordyceps militaris) on Hyperglycemia and Dyslipidemia in Type 2 Diabetic db", db Mice, 2012, pp.21(4), 1157~1162[DOI]
- Reeves PG, "AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet", J Nutr, 123, pp.1939~1951 (1993)
- Matthews DR, "Homeostasis model assessment: insulin resistance and beta-cell function from fasting plasma glucose and insulin concentrations in man", Diabetologia, 28, pp.412~419 (1985)
- Folch J, "A simple method for the isolation and purification of total lipides from animal tissues", J Biol Chem, 226, pp.497~509 (1957)
- Ohkawa H, "Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:351-8. 29. Ellman GL. Tissue sulfhydryl groups", Arch Biochem Biophys, 82, pp.70~77 (1959)
- Bradford MM, "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of proteindye binding", Anal Biochem, 72, pp.248~254 (1976)
- Halaas JL, "Weight-reducing effects of the plasma protein encoded by the obese gene", Science, 269, pp.543~546 (1995)
- Koteish A, "Animal models of steatohepatitis", Best Pract Res Clin Gastroenterol, 16, pp.679~690 (2002)
- Muniyappa R, "Current approaches for assessing insulin sensitivity and resistance in vivo: advantages, limitations, and appropriate usage", AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 294(1), pp.15~26 (2008), AMER PHYSIOLOGICAL SOC
- Choi SB, "Improvement of insulin resistance and insulin secretion by water extracts of Cordyceps militaris, Phellinus linteus, and Paecilomyces tenuipes in 90% pancreatectomized rats", Biosci Biotechnol Biochem, 68, pp.2257~2264 (2004)
- Jensen MD, "Influence of body fat distribution on free fatty acid metabolism in obesity", J Clin Invest, 83, pp.1168~1173 (1989)
- Donnelly KL, "Sources of fatty acids stored in liver and secreted via lipoproteins in patients with nonalcoholic fatty liver disease", J Clin Invest, 115, pp.1343~1351 (2005)
- Gauthier MS, "Concurrent exercise prevents high-fat-diet-induced macrovesicular hepatic steatosis", J Appl Physiol (1985), 94, pp.2127~2134 (2003)
- Hotamisligil GS, "Tumor necrosis factor alpha: a key component of the obesity-diabetes link", Diabetes, 43, pp.1271~1278 (1994)
- Senn JJ, "Suppressor of cytokine signaling-3 (SOCS-3), a potential mediator of interleukin-6-dependent insulin resistance in hepatocytes", J Biol Chem, 278, pp.13740~13746 (2003)
- Kanda H, "MCP-1 contributes to macrophage infiltration into adipose tissue, insulin resistance, and hepatic steatosis in obesity", J Clin Invest, 116, pp.1494~1505 (2006)
- Yang S, "Mitochondrial adaptations to obesity-related oxidant stress", Arch Biochem Biophys, 378, pp.259~268 (2000)
- Ramesh T, "Cordycepin (3'-deoxyadenosine) attenuates age-related oxidative stress and ameliorates antioxidant capacity in rats", Exp Gerontol, 47, pp.979~987 (2012)
- Wild AC, "Regulation of gamma-glutamylcysteine synthetase subunit gene expression by the transcription factor Nrf2", J Biol Chem, 274, pp.33627~33636 (1999)
- Townsend DM, "The importance of glutathione in human disease", Biomed Pharmacother, 57, pp.145~155 (2003)