Effects of ingredients of Korean brown rice cookies on attenuation of cholesterol level and oxidative stress in high-fat diet-fed mice
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BACKGROUND/OBJECTIVES: Owing to health concerns related to the consumption of traditional snacks high in sugars and fats, much effort has been made to develop functional snacks with low calorie content. In this study, a new recipe for Korean rice cookie, dasik, was developed and its antioxidative, lipid-lowering, and anti-inflammatory effects and related mechanisms were elucidated. The effects were compared with those of traditional rice cake dasik (RCD), the lipid-lowering effect of which is greater than that of traditional western-style cookies. MATERIALS/METHODS: Ginseng-added brown rice dasik (GBRD) was prepared with brown rice flour, fructooligosaccharide, red ginseng extract, and propolis. Mice were grouped (n = 7 per group) into those fed a normal AIN-76 diet, a high-fat diet (HFD), and HFD supplemented with RCD or GBRD. Dasik in the HFD accounted for 7% of the total calories. The lipid, reactive oxygen species, and peroxynitrite levels, and degree of lipid peroxidation in the plasma or liver were determined. The expression levels of proteins involved in lipid metabolism and inflammation, and those of antioxidant enzymes were determined by western blot analysis. RESULTS: The plasma and hepatic total cholesterol concentrations in the GBRD group were significantly decreased via downregulation of sterol regulatory element-binding protein-2 and 3-hydroxy-3-methylglutaryl-CoA reductase (P < 0.05). The hepatic peroxynitrite level was significantly lower, whereas glutathione was higher, in the GBRD group than in the RCD group. Among the antioxidant enzymes, catalase (CAT) and glutathione peroxidase (GPx) were significantly upregulated in the GBRD group (P < 0.05). In addition, nuclear factor-kappaB (NF-κB) expression in the GBRD group was significantly lower than that in the RCD group. CONCLUSIONS: GBRD decreases the plasma and hepatic cholesterol levels by downregulating cholesterol synthesis. This new dasik recipe also improves the antioxidative and anti-inflammatory status in HFD-fed mice via CAT and GPx upregulation and NF-κB downregulation. These effects were significantly higher than those of RCD.
Abstract
BACKGROUND/OBJECTIVES: Owing to health concerns related to the consumption of traditional snacks high in sugars and fats, much effort has been made to develop functional snacks with low calorie content. In this study, a new recipe for Korean rice cookie, dasik, was developed and its antioxidative, lipid-lowering, and anti-inflammatory effects and related mechanisms were elucidated. The effects were compared with those of traditional rice cake dasik (RCD), the lipid-lowering effect of which is greater than that of traditional western-style cookies. MATERIALS/METHODS: Ginseng-added brown rice dasik (GBRD) was prepared with brown rice flour, fructooligosaccharide, red ginseng extract, and propolis. Mice were grouped (n = 7 per group) into those fed a normal AIN-76 diet, a high-fat diet (HFD), and HFD supplemented with RCD or GBRD. Dasik in the HFD accounted for 7% of the total calories. The lipid, reactive oxygen species, and peroxynitrite levels, and degree of lipid peroxidation in the plasma or liver were determined. The expression levels of proteins involved in lipid metabolism and inflammation, and those of antioxidant enzymes were determined by western blot analysis. RESULTS: The plasma and hepatic total cholesterol concentrations in the GBRD group were significantly decreased via downregulation of sterol regulatory element-binding protein-2 and 3-hydroxy-3-methylglutaryl-CoA reductase (P < 0.05). The hepatic peroxynitrite level was significantly lower, whereas glutathione was higher, in the GBRD group than in the RCD group. Among the antioxidant enzymes, catalase (CAT) and glutathione peroxidase (GPx) were significantly upregulated in the GBRD group (P < 0.05). In addition, nuclear factor-kappaB (NF-κB) expression in the GBRD group was significantly lower than that in the RCD group. CONCLUSIONS: GBRD decreases the plasma and hepatic cholesterol levels by downregulating cholesterol synthesis. This new dasik recipe also improves the antioxidative and anti-inflammatory status in HFD-fed mice via CAT and GPx upregulation and NF-κB downregulation. These effects were significantly higher than those of RCD.
참고문헌 (46)
- Hong SH, "Rice cookie decreases plasma and hepatic lipid levels in high-fat diet-fed mice: a comparison study with traditional western style cookies", J Food Nutr Res, 5, pp.451~457 (2017)
- 홍선희, "The amelioration of plasma lipids by Korean traditional confectionery in middle-aged women: A cross-over study with western cookie", Nutrition Research and Practice, 10(6), pp.590~596 (2016), 한국영양학회
- Fardet A, "New hypotheses for the health-protective mechanisms of whole-grain cereals: what is beyond fibre?", Nutr Res Rev, 23, pp.65~134 (2010)
- Johnson RJ, "Potential role of sugar (fructose) in the epidemic of hypertension, obesity and the metabolic syndrome, diabetes, kidney disease, and cardiovascular disease", Am J Clin Nutr, 86, pp.899~906 (2007)
- Dominguez AL, "An overview of the recent developments on fructooligosaccharide production and applications", Food Bioproc Tech, 7, pp.324~337 (2014)
- Delgado GT, "The putative effects of prebiotics as immunomodulatory agents", Food Res Int, 44, pp.3167~3173 (2011)
- Merino-Aguilar H, "Effect of fructooligosaccharides fraction from Psacalium decompositum on inflammation and dyslipidemia in rats with fructose-induced obesity", Nutrients, 6, pp.591~604 (2014)
- Nakamura Y, "Fructooligosaccharides suppress high-fat diet-induced fat accumulation in C57BL", 6J mice, 2017, pp.43, 145~151
- Han JY, "Red ginseng extract attenuates kainate-induced excitotoxicity by antioxidative effects", Evid Based Complement Alternat Med, 2012, pp.479016~ (2012)
- Yu T, "Ginsenoside Rc from Korean red ginseng (Panax ginseng C.A. Meyer) attenuates inflammatory symptoms of gastritis, hepatitis and arthritis", Am J Chin Med, 44, pp.595~615 (2016)
- NAGARHARSHA, "Rg3-enriched Korean Red Ginseng enhances blood pressure stability in spontaneously hypertensive rats", Integrative Medicine Research, 5(3), pp.223~229 (2016), 한국한의학연구원[DOI]
- Song YB, "Lipid metabolic effect of Korean red ginseng extract in mice fed on a high-fat diet", J Sci Food Agric, 92, pp.388~396 (2012)
- 방향주, "Korean Red Ginseng Improves Glucose Control in Subjects with Impaired Fasting Glucose, Impaired Glucose Tolerance, or Newly Diagnosed Type 2 Diabetes Mellitus", Journal of Medicinal Food, 17(1), pp.128~134 (2014), 한국식품영양과학회[DOI]
- Kim EJ, "Korean red ginseng extract reduces hypoxia-induced epithelial-mesenchymal transition by repressing NF-κB and ERK1", 2 pathways in colon cancer, 2017, ~
- Watts GF, "Lipid-lowering trials in the primary and secondary prevention of coronary heart disease: new evidence, implications and outstanding issues", Curr Opin Lipidol, 7, pp.341~355 (1996)
- Svegliati-Baroni G, "Glucagon-like peptide-1 receptor activation stimulates hepatic lipid oxidation and restores hepatic signalling alteration induced by a high-fat diet in nonalcoholic steatohepatitis", Liver Int, 31, pp.1285~1297 (2011)
- Peairs AT, "Inflammatory response to a high-fat, low-carbohydrate weight loss diet: effect of antioxidants", Obesity (Silver Spring), 16, pp.1573~1578 (2008)
- Sinha-Hikim I, "A novel cystine based antioxidant attenuates oxidative stress and hepatic steatosis in diet-induced obese mice", Exp Mol Pathol, 91, pp.419~428 (2011)
- Yogalakshmi B, "Grape seed proanthocyanidins and metformin act by different mechanisms to promote insulin signaling in rats fed high calorie diet", J Cell Commun Signal, 8, pp.13~22 (2014)
- Raval J, "Basal reactive oxygen species determine the susceptibility to apoptosis in cirrhotic hepatocytes", Free Radic Biol Med, 41, pp.1645~1654 (2006)
- Kohen R, "Oxidation of biological systems: oxidative stress phenomena, antioxidants, redox reactions, and methods for their quantification", Toxicol Pathol, 30, pp.620~650 (2002)
- Folch J, "A simple method for the isolation and purification of total lipides from animal tissues", J Biol Chem, 226, pp.497~509 (1957)
- Ohkawa H, "Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction", Anal Biochem, 95, pp.351~358 (1979)
- Teare JP, "Automated spectrophotometric method for determining oxidized and reduced glutathione in liver", Clin Chem, 39, pp.686~689 (1993)
- Kooy NW, "Peroxynitritemediated oxidation of dihydrorhodamine 123", Free Radic Biol Med, 16, pp.149~156 (1994)
- 정고은, "Antiatherogenic Effects of Korean Cabbage Kimchi with Added Short Arm Octopus", Food Science and Biotechnology, 24(1), pp.249~255 (2015), 한국식품과학회[DOI]
- Horton JD, "SREBPs: activators of the complete program of cholesterol and fatty acid synthesis in the liver", J Clin Invest, 109, pp.1125~1131 (2002)
- Cao K, "Hydroxytyrosol prevents diet-induced metabolic syndrome and attenuates mitochondrial abnormalities in obese mice", Free Radic Biol Med, 67, pp.396~407 (2014)
- Biddinger SB, "Effects of diet and genetic background on sterol regulatory element-binding protein-1c, stearoyl-CoA desaturase 1, and the development of the metabolic syndrome", Diabetes, 54, pp.1314~1323 (2005)
- Brown MS, "The SREBP pathway: regulation of cholesterol metabolism by proteolysis of a membrane-bound transcription factor", Cell, 89, pp.331~340 (1997)
- Crabb DW, "Alcohol and lipid metabolism", J Gastroenterol Hepatol, 21(Suppl 3), pp.S56~S60 (2006)
- Wong J, "SREBP-2 positively regulates transcription of the cholesterol efflux gene, ABCA1, by generating oxysterol ligands for LXR", Biochem J, 400, pp.485~491 (2006)
- Yang JL, "Barley β-glucan lowers serum cholesterol based on the up-regulation of cholesterol 7α-hydroxylase activity and mRNA abundance in cholesterol-fed rats", J Nutr Sci Vitaminol (Tokyo), 49, pp.381~387 (2003)
- Rong N, "Oryzanol decreases cholesterol absorption and aortic fatty streaks in hamsters", Lipids, 32, pp.303~309 (1997)
- Miura D, "Hypocholesterolemic action of pre-germinated brown rice in hepatoma-bearing rats", Life Sci, 79, pp.259~264 (2006)
- Shen L, "Ginsenoside Rb1 reduces fatty liver by activating AMP-activated protein kinase in obese rats", J Lipid Res, 54, pp.1430~1438 (2013)
- Quan HY, "Ginsenoside Re lowers blood glucose and lipid levels via activation of AMP-activated protein kinase in HepG2 cells and high-fat diet fed mice", Int J Mol Med, 29, pp.73~80 (2012)
- 박재홍, "Antihyperlipidemic Effect of Ginsenoside Rg1 in Type 2 Diabetic Mice", 생명과학회지, 21(7), pp.932~938 (2011), 한국생명과학회
- Lee S, "Ginsenoside Rg3 reduces lipid accumulation with AMP-Activated Protein Kinase (AMPK)activation in HepG2 cells", Int J Mol Sci, 13, pp.5729~5739 (2012)
- Kawase A, "Increased effects of ginsenosides on the expression of cholesterol 7α-hydroxylase but not the bile salt export pump are involved in cholesterol metabolism", J Nat Med, 67, pp.545~553 (2013)
- Kim CS, "Effect of Korea red ginseng on cerebral blood flow and superoxide production", Acta Pharmacol Sin, 23, pp.1152~1156 (2002)
- 김영호, "Effect of Korean Red Ginseng on Testicular Tissue Injury after Torsion and Detorsion", Investigative and Clinical Urology, 51(11), pp.794~799 (2010), 대한비뇨기과학회
- Yong-Seong Kim, "Protective Effect of Korean Red Ginseng against Aflatoxin B_1-Induced Hepatotoxicity in Rat", Journal of Ginseng Research, 35(2), pp.243~249 (2011), 고려인삼학회
- Ramesh T, "Panax ginseng reduces oxidative stress and restores antioxidant capacity in aged rats", Nutr Res, 32, pp.718~726 (2012)
- Sun BS, "Simultaneous quantification of 19 ginsenosides in black ginseng developed from Panax ginseng by HPLC-ELSD", J Pharm Biomed Anal, 50, pp.15~22 (2009)
- Parrado J, "Prevention of brain protein and lipid oxidation elicited by a water-soluble oryzanol enzymatic extract derived from rice bran", Eur J Nutr, 42, pp.307~314 (2003)