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Protective effects of perilla oil and alpha linolenic acid on SH-SY5Y neuronal cell death induced by hydrogen peroxide

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BACKGROUND/OBJECTIVE: Oxidative stress plays a key role in neuronal cell damage, which is associated with neurodegenerative disease. The aim of present study was to investigate the neuroprotective effects of perilla oil (PO) and its active component, alpha-linolenic acid (ALA), against hydrogen peroxide (H2O2)-induced oxidative stress in SH-SY5Y neuronal cells. MATERIALS/METHODS: The SH-SY5Y human neuroblastoma cells exposed to 250 μM H2O2 for 24 h were treated with different concentrations of PO (25, 125, 250 and 500 μg/mL) and its major fatty acid, ALA (1, 2.5, 5 and 25 μg/mL). We examined the effects of PO and ALA on H2O2-induced cell viability, lactate dehydrogenase (LDH) release, and nuclear condensation. Moreover, we determined whether PO and ALA regulated the apoptosis-related protein expressions, such as cleaved-poly ADP ribose polymerase (PARP), cleaved caspase-9 and -3, BCL-2 and BAX. RESULTS: Treatment of H2O2 resulted in decreased cell viability, increased LDH release, and increase in the nuclei condensation as indicated by Hoechst 33342 staining. However, PO and ALA treatment significantly attenuated the neuronal cell death, indicating that PO and ALA potently blocked the H2O2-induced neuronal apoptosis. Furthermore, cleaved-PARP, cleaved caspase-9 and -3 activations were significantly decreased in the presence of PO and ALA, and the H2O2-mediated up-regulated BAX/BCL-2 ratio was blocked after treatment with PO and ALA. CONCLUSIONS: PO and its main fatty acid, ALA, exerted the protective activity from neuronal oxidative stress induced by H2O2. They regulated apoptotic pathway in neuronal cell death by alleviation of BAX/BCL-2 ratio, and down-regulation of cleaved-PARP and cleaved caspase-9 and -3. Although further studies are required to verify the protective mechanisms of PO and ALA from neuronal damage, PO and ALA are the promising agent against oxidative stress-induced apoptotic neuronal cell death.

Abstract

BACKGROUND/OBJECTIVE: Oxidative stress plays a key role in neuronal cell damage, which is associated with neurodegenerative disease. The aim of present study was to investigate the neuroprotective effects of perilla oil (PO) and its active component, alpha-linolenic acid (ALA), against hydrogen peroxide (H2O2)-induced oxidative stress in SH-SY5Y neuronal cells. MATERIALS/METHODS: The SH-SY5Y human neuroblastoma cells exposed to 250 μM H2O2 for 24 h were treated with different concentrations of PO (25, 125, 250 and 500 μg/mL) and its major fatty acid, ALA (1, 2.5, 5 and 25 μg/mL). We examined the effects of PO and ALA on H2O2-induced cell viability, lactate dehydrogenase (LDH) release, and nuclear condensation. Moreover, we determined whether PO and ALA regulated the apoptosis-related protein expressions, such as cleaved-poly ADP ribose polymerase (PARP), cleaved caspase-9 and -3, BCL-2 and BAX. RESULTS: Treatment of H2O2 resulted in decreased cell viability, increased LDH release, and increase in the nuclei condensation as indicated by Hoechst 33342 staining. However, PO and ALA treatment significantly attenuated the neuronal cell death, indicating that PO and ALA potently blocked the H2O2-induced neuronal apoptosis. Furthermore, cleaved-PARP, cleaved caspase-9 and -3 activations were significantly decreased in the presence of PO and ALA, and the H2O2-mediated up-regulated BAX/BCL-2 ratio was blocked after treatment with PO and ALA. CONCLUSIONS: PO and its main fatty acid, ALA, exerted the protective activity from neuronal oxidative stress induced by H2O2. They regulated apoptotic pathway in neuronal cell death by alleviation of BAX/BCL-2 ratio, and down-regulation of cleaved-PARP and cleaved caspase-9 and -3. Although further studies are required to verify the protective mechanisms of PO and ALA from neuronal damage, PO and ALA are the promising agent against oxidative stress-induced apoptotic neuronal cell death.

참고문헌 (51)

  1. Jacobson MD, "Reactive oxygen species and programmed cell death", Trends Biochem Sci, 21, pp.83~86 (1996)[DOI]
  2. Halliwell B, "DNA damage by oxygen-derived species. Its mechanism and measurement in mammalian systems", FEBS Lett, 281, pp.9~19 (1991)[DOI]
  3. Braughler JM, "Central nervous system trauma and stroke. I. Biochemical considerations for oxygen radical formation and lipid peroxidation", Free Radic Biol Med, 6, pp.289~301 (1989)[DOI]
  4. Moreira PI, "Oxidative stress: the old enemy in Alzheimer's disease pathophysiology", Curr Alzheimer Res, 2, pp.403~408 (2005)[DOI]
  5. Xue S, "Hypoxia and reoxygenation increased BACE1 mRNA and protein levels in human neuroblastoma SH-SY5Y cells", Neurosci Lett, 405, pp.231~235 (2006)[DOI]
  6. Zheng L, "Autophagy of amyloid beta-protein in differentiated neuroblastoma cells exposed to oxidative stress", Neurosci Lett, 394, pp.184~189 (2006)[DOI]
  7. Yadav NS, "Cloning of higher plant omega-3 fatty acid desaturases", Plant Physiol, 103, pp.467~476 (1993)
  8. Oliver MF, "It is more important to increase the intake of unsaturated fats than to decrease the intake of saturated fats: evidence from clinical trials relating to ischemic heart disease", Am J Clin Nutr, 66, pp.980S~986S (1997)
  9. Calder PC, "n-3 polyunsaturated fatty acids, inflammation, and inflammatory diseases", Am J Clin Nutr, 83, pp.1505S~1519S (2006)
  10. Butterfield DA, "Roles of amyloid <TEX>${\beta}$<", TEX>-peptide-associated oxidative stress and brain protein modifications in the pathogenesis of Alzheimer's disease and mild cognitive impairment, 2007, pp.43, 658~677
  11. 이아영, "Effects of Vegetable Oils with Different Fatty Acid Compositions on Cognition and Memory Ability in Aβ25–35-Induced Alzheimer's Disease Mouse Model", Journal of Medicinal Food, 19(10), pp.912~921 (2016), 한국식품영양과학회[DOI]
  12. Lee AY, "Alpha-linolenic acid from Perilla frutescens var. japonica oil protects <TEX>$A{\beta}$<", TEX>-induced cognitive impairment through regulation of APP processing and <TEX>$A{\beta}$<, J Agric Food Chem, pp.2017, 65[DOI]
  13. Skowyra M, "The effect of perilla frutescens extract on the oxidative stability of model food emulsions", Antioxidants (Basel), 3, pp.38~54 (2014)[DOI]
  14. Hassan A, "An <TEX>${\alpha}$<", TEX>-linolenic acid-rich formula reduces oxidative stress and inflammation by regulating <TEX>$NF-{\kappa}B$<, J Nutr, pp.2010, 140[DOI]
  15. Seong J, "Perilla oil rich in <TEX>${\alpha}$<", TEX>-linolenic acid inhibits neuronal apoptosis and the expression of inflammation-mediator protein in apoE KO mice, 2012, pp.1, 167~173
  16. Fahn S, "The oxidant stress hypothesis in Parkinson's disease: evidence supporting it", Ann Neurol, 32, pp.804~812 (1992)[DOI]
  17. 이재민, "Fatty Acid Content in Perilla Cultivars and Commercial Oils Determined by GC Analysis", Natural Product Sciences, 22(4), pp.259~262 (2016), 한국생약학회
  18. 이재민, "High-yield methods for purification of a-linolenic acid from Perilla frutescens var. japonica oil", Applied Biological Chemistry, 59(1), pp.89~94 (2016), 한국응용생명화학회
  19. Cory AH, "Use of an aqueous soluble tetrazolium", formazan assay for cell growth assays in culture, 1991, pp.3, 207~212
  20. Racher AJ, "Use of lactate dehydrogenase release to assess changes in culture viability", Cytotechnology, 3, pp.301~307 (1990)[DOI]
  21. Xiao GH, "Anti-apoptotic signaling by hepatocyte growth factor", met via the phosphatidylinositol 3-kinase, Proc Natl Acad Sci U S A, pp.2001, 98[DOI]
  22. Arias E, "Unequal neuroprotection afforded by the acetylcholinesterase inhibitors galantamine, donepezil, and rivastigmine in SH-SY5Y neuroblastoma cells: role of nicotinic receptors", J Pharmacol Exp Ther, 315, pp.1346~1353 (2005)[DOI]
  23. Lopes JP, "Role of cyclin-dependent kinase 5 in the neurodegenerative process triggered by amyloid-beta and prion peptides: implications for Alzheimer's disease and prion-related encephalopathies", Cell Mol Neurobiol, 27, pp.943~957 (2007)[DOI]
  24. Shibata N, "The role for oxidative stress in neurodegenerative diseases", Brain Nerve, 60, pp.157~170 (2008)
  25. Aruoma OI, "Neuroprotection by bioactive components in medicinal and food plant extracts", Mutat Res, 544, pp.203~215 (2003)[DOI]
  26. Kitagishi Y, "Dietary regulation of PI3K", AKT, TEX> pathway in Alzheimer's disease, pp.Alzheimers Res Ther, 2014[DOI]
  27. Zheng WX, "Baicalin protects PC-12 cells from oxidative stress induced by hydrogen peroxide via anti-apoptotic effects", Brain Inj, 28, pp.227~234 (2014)[DOI]
  28. Palacios-pelaez R, "Omega-3 essential fatty acids modulate initiation and progression of neurodegenerative disease", Mol Neurobiol, 41, pp.367~374 (2010)[DOI]
  29. 정근희, "Effects of perilla oil on plasma concentrations of cardioprotective (n-3) fatty acids and lipid profiles in mice", Nutrition Research and Practice, 7(4), pp.256~261 (2013), 한국영양학회[DOI]
  30. Gerster H, "Can adults adequately convert alpha-linolenic acid (18:3n-3) to eicosapentaenoic acid (20:5n-3) and docosahexaenoic acid (22:6n-3)?", Int J Vitam Nutr Res, 68, pp.159~173 (1998)
  31. Pawlosky RJ, "Physiological compartmental analysis of <TEX>${\alpha}$<", TEX>-linolenic acid metabolism in adult humans, 2001, pp.42, 1257~1265
  32. Blondeau N, "Innate Tolerance in the CNS", pp.133~163 (2013), Springer
  33. Ja Young Jang, "Perilla oil improves blood flow through inhibition of platelet aggregation and thrombus formation", Laboratory Animal Research, 30(1), pp.21~27 (2014), 한국실험동물학회
  34. Senavong P, "Neuroprotective effect of perilla extracts on PC12 cells", J Med Assoc Thai, 99, pp.S256~S264 (2016)
  35. Lee AY, "Comparative study on antioxidant activity of vegetable oils under in vitro and cellular system", J Agric Sci, 7, pp.58~65 (2015)
  36. Kim SS, "Neuroprotective effects of 3,5-dicaffeoylquinic acid on hydrogen peroxide-induced cell death in SH-SY5Y cells", Phytother Res, 19, pp.243~245 (2005)[DOI]
  37. Chetsawang J, "Hydrogen peroxide toxicity induces Ras signaling in human neuroblastoma SH-SY5Y cultured cells", J Biomed Biotechnol, 2010, pp.803815~ (2010)
  38. Barbosa DS, "Decreased oxidative stress in patients with ulcerative colitis supplemented with fish oil <TEX>$\omega$<", TEX>-3 fatty acids, 2003, pp.19, 837~842
  39. Fotakis G, "In vitro cytotoxicity assays: Comparison of LDH, neutral red, MTT and protein assay in hepatoma cell lines following exposure to cadmium chloride", Toxicol Lett, 160, pp.171~177 (2006)[DOI]
  40. Wang P, "Limitation of MTT and MTS-based assays for measurement of antiproliferative activity of green tea polyphenols", PLoS One, 5, pp.e10202~ (2010)[DOI]
  41. Enari M, "A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD", Nature, 391, pp.43~50 (1998)[DOI]
  42. Kwon SH, "Eucommia ulmoides Oliv. Bark. protects against hydrogen peroxide-induced neuronal cell death in SH-SY5Y cells", J Ethnopharmacol, 142, pp.337~345 (2012)
  43. Hu XL, "Neuroprotective effects of Kukoamine B against hydrogen peroxide-induced apoptosis and potential mechanisms in SH-SY5Y cells", Environ Toxicol Pharmacol, 40, pp.230~240 (2015)[DOI]
  44. Youle RJ, "The Bcl-2 protein family: opposing activities that mediate cell death", Nat Rev Mol Cell Biol, 9, pp.47~59 (2008)[DOI]
  45. Reed JC, "Double identity for proteins of the Bcl-2 family", Nature, 387, pp.773~776 (1997)[DOI]
  46. Mattson MP, "Apoptosis in neurodegenerative disorders", Nat Rev Mol Cell Biol, 1, pp.120~130 (2000)[DOI]
  47. Murphy KM, "Bcl-2 inhibits Bax translocation from cytosol to mitochondria during drug-induced apoptosis of human tumor cells", Cell Death Differ, 7, pp.102~111 (2000)[DOI]
  48. Grutter MG, "Caspases: key players in programmed cell death", Curr Opin Struct Biol, 10, pp.649~655 (2000)[DOI]
  49. Egert S, "Dietary alpha-linolenic acid, EPA, and DHA have differential effects on LDL fatty acid composition but similar effects on serum lipid profiles in normolipidemic humans", J Nutr, 139, pp.861~868 (2009)
  50. Zhao G, "Anti-inflammatory effects of polyunsaturated fatty acids in THP-1 cells", Biochem Biophys Res Commun, 336, pp.909~917 (2005)[DOI]
  51. Albert CM, "Dietary <TEX>${\alpha}$<", TEX>-linolenic acid intake and risk of sudden cardiac death and coronary heart disease, 2005, pp.112, 3232~3238

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