본문 바로가기
KERD
논문KCI 등재

Standardized rice bran extract improves hepatic steatosis in HepG2 cells and ovariectomized rats

4

조회

0

다운로드

1

인용

0

참고문헌

키워드

초록

BACKGROUD/OBJECTIVES: Hepatic steatosis is the most common liver disorder, particularly in postmenopausal women. This study investigated the protective effects of standardized rice bran extract (RBS) on ovariectomized (OVX)-induced hepatic steatosis in rats. MATERIALS/METHODS: HepG2 cells were incubated with 200 µM oleic acid to induce lipid accumulation with or without RBS and γ-oryzanol. OVX rats were separated into three groups and fed a normal diet (ND) or the ND containing 17β-estradiol (E2; 10 µg/kg) and RBS (500 mg/kg) for 16 weeks. RESULTS: RBS supplementation improved serum triglyceride and free fatty acid levels in OVX rats. Histological analysis showed that RBS significantly attenuated hepatic fat accumulation and decreased hepatic lipid, total cholesterol, and triglyceride levels. Additionally, RBS suppressed the estrogen deficiency-induced upregulation of lipogenic genes, such as sterol regulatory element-binding protein 1 (SREBP1), acetyl-CoA carboxylase 1, fatty acid synthase, glycerol-3-phosphate acyltransferase, and stearoyl-CoA desaturase 1. CONCLUSIONS: RBS and γ-oryzanol effectively reduced lipid accumulation in a HepG2 cell hepatic steatosis model. RBS improves OVX-induced hepatic steatosis by regulating the SREBP1-mediated activation of lipogenic genes, suggesting the benefits of RBS in preventing fatty liver in postmenopausal women.

Abstract

BACKGROUD/OBJECTIVES: Hepatic steatosis is the most common liver disorder, particularly in postmenopausal women. This study investigated the protective effects of standardized rice bran extract (RBS) on ovariectomized (OVX)-induced hepatic steatosis in rats. MATERIALS/METHODS: HepG2 cells were incubated with 200 µM oleic acid to induce lipid accumulation with or without RBS and γ-oryzanol. OVX rats were separated into three groups and fed a normal diet (ND) or the ND containing 17β-estradiol (E2; 10 µg/kg) and RBS (500 mg/kg) for 16 weeks. RESULTS: RBS supplementation improved serum triglyceride and free fatty acid levels in OVX rats. Histological analysis showed that RBS significantly attenuated hepatic fat accumulation and decreased hepatic lipid, total cholesterol, and triglyceride levels. Additionally, RBS suppressed the estrogen deficiency-induced upregulation of lipogenic genes, such as sterol regulatory element-binding protein 1 (SREBP1), acetyl-CoA carboxylase 1, fatty acid synthase, glycerol-3-phosphate acyltransferase, and stearoyl-CoA desaturase 1. CONCLUSIONS: RBS and γ-oryzanol effectively reduced lipid accumulation in a HepG2 cell hepatic steatosis model. RBS improves OVX-induced hepatic steatosis by regulating the SREBP1-mediated activation of lipogenic genes, suggesting the benefits of RBS in preventing fatty liver in postmenopausal women.

참고문헌 (49)

  1. Farrell GC, "Nonalcoholic fatty liver disease : from steatosis to cirrhosis", Hepatology, 43, pp.S99~S112 (2006)
  2. Singh A, "The utility of noninvasive scores in assessing the prevalence of nonalcoholic fatty liver disease and advanced fibrosis in type 1 diabetic patients", Hepatol Int, 12, pp.37~43 (2018)
  3. Fan JG, "New trends on obesity and NAFLD in Asia", J Hepatol, 67, pp.862~873 (2017)
  4. Summart U, "Gender differences in the prevalence of nonalcoholic fatty liver disease in the Northeast of Thailand : a population-based cross-sectional study", F1000 Res, 6, pp.1630~ (2017)
  5. Fu X, "Treatment with estrogen protects against ovariectomy-induced hepatic steatosis by increasing AQP7 expression", Mol Med Rep, 14, pp.425~431 (2016)
  6. Gualtieri M, "Surgical menopause and the increased risk of non-alcoholic fatty liver disease in patients with endometrial hyperplasia and cancer", Fertil Steril, 103, pp.e23~ (2015)
  7. Walsh BW, "Effects of postmenopausal estrogen replacement on the concentrations and metabolism of plasma lipoproteins", N Engl J Med, 325, pp.1196~1204 (1991)
  8. Darine Froy N. Mabunga, "Treatment of GABA from Fermented Rice Germ Ameliorates Caffeine-Induced Sleep Disturbance in Mice", Biomolecules & Therapeutics, 23(3), pp.268~274 (2015), 한국응용약물학회[DOI]
  9. Wani AA, "Rice starch diversity : effects on structural, morphological, thermal, and physicochemical properties-a review", Compr Rev Food Sci Food Saf, 11, pp.417~436 (2012)
  10. Laokuldilok T, "Antioxidants and antioxidant activity of several pigmented rice brans", J Agric Food Chem, 59, pp.193~199 (2011)
  11. Kahlon TS, "Cholesterol-lowering in hamsters fed rice bran at various levels, defatted rice bran and rice bran oil", J Nutr, 122, pp.513~519 (1992)
  12. Rhee YH, "Anti-oxidant and anti-inflammatory effects of rice bran and green tea fermentation mixture on lipopolysaccharide-induced RAW 264. 7 macrophages", Trop J Pharm Res, 16, pp.2943~2951 (2017)
  13. Nie Y, "Anti-hyperlipidemic effect of rice bran polysaccharide and its potential mechanism in high-fat diet mice", Food Funct, 8, pp.4028~4041 (2017)
  14. Qureshi AA, "Effects of stabilized rice bran, its soluble and fiber fractions on blood glucose levels and serum lipid parameters in humans with diabetes mellitus types I and II", J Nutr Biochem, 13, pp.175~187 (2002)
  15. Cheng HH, "Ameliorative effects of stabilized rice bran on type 2 diabetes patients", Ann Nutr Metab, 56, pp.45~51 (2010)
  16. Muhammad SI, "Upregulation of genes related to bone formation by γ-amino butyric acid and γ-oryzanol in germinated brown rice is via the activation of GABAB-receptors and reduction of serum IL-6 in rats", Clin Interv Aging, 8, pp.1259~1271 (2013)
  17. Mastinu A, "Gamma-oryzanol prevents LPS-induced brain inflammation and cognitive impairment in adult mice", Nutrients, 11, pp.728~ (2019)
  18. Høegh-Andersen P, "Ovariectomized rats as a model of postmenopausal osteoarthritis : validation and application", Arthritis Res Ther, 6, pp.R169~R180 (2004)
  19. Yang H, "Sleep-promoting effects and possible mechanisms of action associated with a standardized rice bran supplement", Nutrients, 9, pp.512~ (2017)
  20. Um MY, "A novel neurological function of rice bran : a standardized rice bran supplement promotes non-rapid eye movement sleep in mice through histamine H1 receptors", Mol Nutr Food Res, 61, pp.1700316~ (2017)
  21. Um MY, "Rice bran extract supplement improves sleep efficiency and sleep onset in adults with sleep disturbance : a randomized, double-blind, placebo-controlled, polysomnographic study", Sci Rep, 9, pp.12339~ (2019)
  22. Lim DW, "Effects of dietary isoflavones from Puerariae radix on lipid and bone metabolism in ovariectomized rats", Nutrients, 5, pp.2734~2746 (2013)
  23. Lim DW, "Anti-osteoporotic effects of Angelica sinensis(Oliv. )Diels extract on ovariectomized rats and its oral toxicity in rats", Nutrients, 6, pp.4362~4372 (2014)
  24. Folch J, "A simple method for the isolation and purification of total lipides from animal tissues", J Biol Chem, 226, pp.497~509 (1957)
  25. Sawada K, "Absorption and metabolism of γ-oryzanol, a characteristic functional ingredient in rice bran", J Nutr Sci Vitaminol(Tokyo), 65, pp.S180~S184 (2019)
  26. Jones ME, "Aromatase-deficient(ArKO)mice have a phenotype of increased adiposity", Proc Natl Acad Sci U S A, 97, pp.12735~12740 (2000)
  27. Weigt C, "Effects of estradiol, estrogen receptor subtype-selective agonists and genistein on glucose metabolism in leptin resistant female Zucker diabetic fatty(ZDF)rats", J Steroid Biochem Mol Biol, 154, pp.12~22 (2015)
  28. Heine PA, "Increased adipose tissue in male and female estrogen receptor-alpha knockout mice", Proc Natl Acad Sci U S A, 97, pp.12729~12734 (2000)
  29. Lavoie JM, "NAFLD, estrogens, and physical exercise : the animal model", J Nutr Metab, 2012, pp.914938~ (2012)
  30. Oliveira MC, "A long-term estrogen deficiency in ovariectomized mice is associated with disturbances in fatty acid oxidation and oxidative stress", Rev Bras Ginecol Obstet, 40, pp.251~259 (2018)
  31. Campos LB, "Cimicifuga racemosa impairs fatty acid β-oxidation and induces oxidative stress in livers of ovariectomized rats with renovascular hypertension", Free Radic Biol Med, 53, pp.680~689 (2012)
  32. Charatcharoenwitthaya P, "The spontaneous course of liver enzymes and its correlation in nonalcoholic fatty liver disease", Dig Dis Sci, 57, pp.1925~1931 (2012)
  33. Chong CL, "Hepatoprotective effects of Morinda citrifolia leaf extract on ovariectomized rats fed with thermoxidized palm oil diet : evidence at histological and ultrastructural level", Oxid Med Cell Longev, 2019 (2019)
  34. Koshy SM, "Amla prevents fructose-induced hepatic steatosis in ovariectomized rats : role of liver FXR and LXRα", Climacteric, 18, pp.299~310 (2015)
  35. Watanabe M, "Bile acids lower triglyceride levels via a pathway involving FXR, SHP, and SREBP-1c", J Clin Invest, 113, pp.1408~1418 (2004)
  36. Kohjima M, "Re-evaluation of fatty acid metabolism-related gene expression in nonalcoholic fatty liver disease", Int J Mol Med, 20, pp.351~358 (2007)
  37. Flowers MT, "Role of stearoyl-coenzyme A desaturase in regulating lipid metabolism", Curr Opin Lipidol, 19, pp.248~256 (2008)
  38. Kotronen A, "Hepatic stearoyl-CoA desaturase(SCD)-1 activity and diacylglycerol but not ceramide concentrations are increased in the nonalcoholic human fatty liver", Diabetes, 58, pp.203~208 (2009)
  39. Cao J, "Molecular identification of microsomal acyl-CoA : glycerol-3-phosphate acyltransferase, a key enzyme in de novo triacylglycerol synthesis", Proc Natl Acad Sci U S A, 103, pp.19695~19700 (2006)
  40. Thuresson ER, "Inhibition of glycerol-3-phosphate acyltransferase as a potential treatment for insulin resistance and type 2 diabetes", Curr Opin Investig Drugs, 5, pp.411~418 (2004)
  41. Panneerselvam S, "Soy isoflavones(Glycine max)ameliorate hypertriglyceridemia and hepatic steatosis in high fat-fed ovariectomized Wistar rats(an experimental model of postmenopausal obesity)", J Nutr Biochem, 38, pp.57~69 (2016)
  42. Kamei Y, "Ovariectomy in mice decreases lipid metabolism-related gene expression in adipose tissue and skeletal muscle with increased body fat", J Nutr Sci Vitaminol(Tokyo), 51, pp.110~117 (2005)
  43. Hao CL, "Pre-germinated brown rice extract ameliorates high-fat diet-induced metabolic syndrome", J Food Biochem, 43, pp.e12769~ (2019)
  44. Perez-Ternero C, "Rice bran enzymatic extract reduces atherosclerotic plaque development and steatosis in high-fat fed ApoE-", -mice, 2017, pp.37, 22~29
  45. Jung TD, "Comparative analysis of γ-oryzanol, β-glucan, total phenolic content and antioxidant activity in fermented rice bran of different varieties", Nutrients, 9, pp.571~ (2017)
  46. Justo ML, "Water-soluble rice bran enzymatic extract attenuates dyslipidemia, hypertension and insulin resistance in obese Zucker rats", Eur J Nutr, 52, pp.789~797 (2013)
  47. Wang O, "Effects of ferulic acid and γ-oryzanol on high-fat and high-fructose diet-induced metabolic syndrome in rats", PLoS One, 10, pp.e0118135~ (2015)
  48. Wang L, "Oryzanol modifies high fat diet-induced obesity, liver gene expression profile, and inflammation response in mice", J Agric Food Chem, 65, pp.8374~8385 (2017)
  49. Song L, "Phytosterol esters attenuate hepatic steatosis in rats with non-alcoholic fatty liver disease rats fed a high-fat diet", Sci Rep, 7, pp.41604~ (2017)

관련 논문AI추천

Fermented Soymilk Alleviates Lipid Accumulation by Inhibition of SREBP-1 and Activation of NRF-2 in the Hepatocellular Steatosis Model김리지, 김지현, 안상봉, 이종훈, 이태복, 전대원, 전진현, Wen Hao Wu한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2018 · 제28권 제2호Hypocholesterolemic metabolism of dietary red pericarp glutinous rice rich in phenolic compounds in mice fed a high cholesterol diet김은혜, 박용순, 박은미, 정일민한국영양학회 · Nutrition Research and Practice2014 · 제8권 제6호Ethanol extract of Allium fistulosum inhibits development of non-alcoholic fatty liver disease박재호, 신은주, 정민유, 정상원, 최효경, 황진택한국영양학회 · Nutrition Research and Practice2018 · 제12권 제2호중만분소환 추출물이 Palmitate로 유발된 비알코올성 지방간 HepG2 cell 모델에 미치는 영향이지원, 전상윤, 최창원, 하예진, 한창우대한한방내과학회 · 대한한방내과학회지2016 · 제37권 제3호고당 및 올레산으로 유도된 HepG2 세포에서 국내산 호두(Juglans regia) 추출물의 비알코올성 지방간 질환 개선 효과이욱, 강진용, 고민지, 김민지, 김종민, 김태윤, 문종현, 이효림, 정혜린, 주승겸, 허호진한국식품저장유통학회 · Food Science and Preservation2022 · 제29권 제5호Downregulation of Hepatic De Novo Lipogenesis and Adipogenesis in Adipocytes by Pinus densiflora Bark Extract고광웅, 김우기, 김화연, 류융선, 민복기, 안혜명, 윤성호, 정정호, Knowledge Mudhibadi Moyo한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2017 · 제27권 제11호

이 간행물의 다른 논문 (9건)