Herbal extract THI improves metabolic abnormality in mice fed a high-fat diet
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초록
Target herbal ingredient (THI) is an extract made from two herbs, Scutellariae Radix and Platycodi Radix. It has been developed as a treatment for metabolic diseases such as hyperlipidemia, atherosclerosis, and hypertension. One component of these two herbs has been reported to have anti-inflammatory, anti-hyperlipidemic, and anti-obesity activities. However, there have been no reports about the effects of the mixed extract of these two herbs on metabolic diseases. In this study, we investigated the metabolic effects of THI using a diet-induced obesity (DIO) mouse model. High-fat diet (HFD) mice were orally administered daily with 250 mg/kg of THI. After 10 weeks of treatment, the THI-administered HFD mice showed reduction of body weights and epididymal white adipose tissue weights as well as improved glucose tolerance. In addition, the level of total cholesterol in the serum was markedly reduced. To elucidate the molecular mechanism of the metabolic effects of THI in vitro, 3T3-L1 cells were treated with THI, after which the mRNA levels of adipogenic transcription factors, including C/EBPα and PPARγ, were measured. The results show that the expression of these two transcription factors was down regulated by THI in a dose-dependent manner. We also examined the combinatorial effects of THI and swimming exercise on metabolic status. THI administration simultaneously accompanied by swimming exercise had a synergistic effect on serum cholesterol levels. These findings suggest that THI could be developed as a supplement for improving metabolic status.
Abstract
Target herbal ingredient (THI) is an extract made from two herbs, Scutellariae Radix and Platycodi Radix. It has been developed as a treatment for metabolic diseases such as hyperlipidemia, atherosclerosis, and hypertension. One component of these two herbs has been reported to have anti-inflammatory, anti-hyperlipidemic, and anti-obesity activities. However, there have been no reports about the effects of the mixed extract of these two herbs on metabolic diseases. In this study, we investigated the metabolic effects of THI using a diet-induced obesity (DIO) mouse model. High-fat diet (HFD) mice were orally administered daily with 250 mg/kg of THI. After 10 weeks of treatment, the THI-administered HFD mice showed reduction of body weights and epididymal white adipose tissue weights as well as improved glucose tolerance. In addition, the level of total cholesterol in the serum was markedly reduced. To elucidate the molecular mechanism of the metabolic effects of THI in vitro, 3T3-L1 cells were treated with THI, after which the mRNA levels of adipogenic transcription factors, including C/EBPα and PPARγ, were measured. The results show that the expression of these two transcription factors was down regulated by THI in a dose-dependent manner. We also examined the combinatorial effects of THI and swimming exercise on metabolic status. THI administration simultaneously accompanied by swimming exercise had a synergistic effect on serum cholesterol levels. These findings suggest that THI could be developed as a supplement for improving metabolic status.
참고문헌 (26)
- Ahn KS, "Inhibition of inducible nitric oxide synthase and cyclooxygenase II by Platycodon grandiflorum saponins via suppression of nuclear factor-KB activation in RAW 264.7 cells", LIFE SCIENCES, 76, pp.2315~2328 (2005), PERGAMON-ELSEVIER SCIENCE LTD
- Choi CY, "Augmentation of macrophage functions by an aqueous extract isolated from Platycodon grandiflorum", Cancer Lett, 166, pp.17~25 (2001)
- Choi CY, "Aqueous extract isolated from Platycodon grandiflorum elicits the release of nitric oxide and tumor necrosis factor-alpha from murine macrophages", Int Immunopharmacol, 1, pp.1141~1151 (2001)
- Kim YP, "Inhibition of prostaglandin E2 production by platycodin D isolated from the root of Platycodon grandiflorum", Planta Med, 67, pp.362~364 (2001)
- Han LK, "Saponins from platycodi radix ameliorate high fat diet-induced obesity in mice", J Nutr, 132, pp.2241~2245 (2002)
- Zhao HL, "Antiobese and hypolipidemic effects of platycodin saponins in diet-induced obese rats: evidences for lipase inhibition and calorie intake restriction", Int J Obes (Lond), 29, pp.983~990 (2005)
- Lee H, "Platycodin D inhibits adipogenesis of 3T3-L1 cells by modulating Kruppel-like factor 2 and peroxisome proliferator-activated receptor gamma", Phytother Res, 24(2), pp.161~167 (2010)
- Krakauer T, "The flavonoid baicalin inhibits superantigen-induced inflammatory cytokines and chemokines", FEBS Lett, 500, pp.52~55 (2001)
- Liu LY, "Protective effects of baicalin on oxygen", glucose deprivationand NMDA-induced injuries in rat hippocampal slices, 2005, pp.57, 1019~1026
- Zhao Y, "Effects of dietary baicalin supplementation on iron overload-induced mouse liver oxidative injury", Eur J Pharmacol, 509, pp.195~200 (2005)
- Lee H, "Antiobesity Effect of Baicalin involves the Modulations of Proadipogenic and Antiadipogenic Regulators of the Adipogenesis Pathway", PHYTOTHERAPY RESEARCH, 23(11), pp.1615~1623, JOHN WILEY SONS LTD
- Lee H, "Beta-Catenin mediates the anti-adipogenic effect of baicalin", Biochem Biophys Res Commun, 398, pp.741~746 (2010)
- Kasturi R, "Hormonal regulation of stearoyl coenzyme A desaturase activity and lipogenesis during adipose conversion of 3T3-L1 cells", J Biol Chem, 257, pp.12224~12230 (1982)
- Rayalam S, "Phytochemicals and regulation of the adipocyte life cycle", J Nutr Biochem, 19, pp.717~726 (2008)
- Hwang JT, "Genistein, EGCG, and capsaicin inhibit adipocyte differentiation process via activating AMP-activated protein kinase", Biochemical and Biophysical Research Communications, 338(2), pp.694~699 (2005), Academic Press
- Bai L, "Modulation of Sirt1 by resveratrol and nicotinamide alters proliferation and differentiation of pig preadipocytes", Mol Cell Biochem, 307, pp.129~140 (2008)
- 차연수, "Transcriptome analysis and promoter sequence studies on early adipogenesis in 3T3-L1 cells", Nutrition Research and Practice, 1(1), pp.19~28 (2007), 한국영양학회
- Tontonoz P, "mPPAR gamma 2: tissue-specific regulator of an adipocyte enhancer", Genes Dev, 8, pp.1224~1234 (1994)
- Rosen ED, "C", EBPalpha induces adipogenesis through PPARgamma: a unified pathway, 2002, pp.16, 22~26
- Hui KM, "Anxiolytic effect of wogonin, a benzodiazepine receptor ligand isolated from Scutellaria baicalensis Georgi", Biochem Pharmacol, 64, pp.1415~1424 (2002)
- Enomoto R, "Wogonin potentiates the antitumor action of etoposide and ameliorates its adverse effects", Cancer Chemother Pharmacol, 67, pp.1063~1072 (2011)
- Nagai T, "Inhibition of mouse liver sialidase by the root of Scutellaria baicalensis", Planta Med, 55, pp.27~29 (1989)
- Li BQ, "The flavonoid baicalin exhibits anti-inflammatory activity by binding to chemokines", Immunopharmacology, 49, pp.295~306 (2000)
- Lixuan Z, "Baicalin attenuates inflammation by inhibiting NF-kappaB activation in cigarette smoke induced inflammatory models", Pulm Pharmacol Ther, 23, pp.411~419 (2010)
- Du G, "Baicalin suppresses lung carcinoma and lung metastasis by SOD mimic and HIF-1alpha inhibition", Eur J Pharmacol, 630, pp.121~130 (2010)
- Kim J, "Effects of gender and prior swim exercise on glucose uptake in isolated skeletal muscles from mice", J Physiol Sci, 56, pp.305~312 (2006)