Lutein decreases oxidative stress and inflammation in liver and eyes of guinea pigs fed a hypercholesterolemic diet
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Guinea pigs were fed a hypercholesterolemic diet (0.25 g/100 g cholesterol) and randomly allocated either to a Control group (n = 9) or to a Lutein (0.1 g/100 g) group (n = 10) for 12 weeks to evaluate oxidative stress and inflammation in both liver and eyes. Malondialdehyde (MDA)concentrations and inflammatory cytokines were measured as well as hepatic nuclear factor-kappaB (NF-κB) binding. Lutein concentrations were greater in eyes (P < 0.01) and liver (P < 0.001) in the Lutein group. All guinea pigs had high concentrations of hepatic cholesterol as well as high plasma ALT and AST levels indicative of liver injury. However, the Lutein group had 43% lower hepatic free cholesterol than the Controls (P < 0.05). Hepatic MDA and MDA in the eye were lower in the Lutein compared to the Control group (P < 0.05). Hepatic tumor necrosis factor-αwas 32% lower in the Lutein group (P < 0.05). Lastly, the Lutein group presented lower NF-κB DNA binding activity than the Control group (P < 0.001). These results suggest that in the presence of high cholesterol, lutein exerts both antioxidant and anti-inflammatory effects, which can be explained by attenuated NF-κB DNA binding activity. Furthermore, results also suggest that lutein accumulates in the eyes of guinea pigs to protect against oxidative stress.
Abstract
Guinea pigs were fed a hypercholesterolemic diet (0.25 g/100 g cholesterol) and randomly allocated either to a Control group (n = 9) or to a Lutein (0.1 g/100 g) group (n = 10) for 12 weeks to evaluate oxidative stress and inflammation in both liver and eyes. Malondialdehyde (MDA)concentrations and inflammatory cytokines were measured as well as hepatic nuclear factor-kappaB (NF-κB) binding. Lutein concentrations were greater in eyes (P < 0.01) and liver (P < 0.001) in the Lutein group. All guinea pigs had high concentrations of hepatic cholesterol as well as high plasma ALT and AST levels indicative of liver injury. However, the Lutein group had 43% lower hepatic free cholesterol than the Controls (P < 0.05). Hepatic MDA and MDA in the eye were lower in the Lutein compared to the Control group (P < 0.05). Hepatic tumor necrosis factor-αwas 32% lower in the Lutein group (P < 0.05). Lastly, the Lutein group presented lower NF-κB DNA binding activity than the Control group (P < 0.001). These results suggest that in the presence of high cholesterol, lutein exerts both antioxidant and anti-inflammatory effects, which can be explained by attenuated NF-κB DNA binding activity. Furthermore, results also suggest that lutein accumulates in the eyes of guinea pigs to protect against oxidative stress.
참고문헌 (40)
- Khachik F, "Lutein, lycopene, and their oxidative metabolites in chemoprevention of cancer", J Cell Biochem Suppl, 22, pp.236~246 (1995)
- Krinsky NI, "Biologic mechanisms of the protective role of lutein and zeaxanthin in the eye", Annu Rev Nutr, 23, pp.171~201 (2003)
- Donoso LA, "The role of inflammation in the pathogenesis of age-related macular degeneration", Surv Ophthalmol, 51, pp.137~152 (2006)
- Hollyfield JG, "Oxidative damageinduced inflammation initiates age-related macular degeneration", Nat Med, 14, pp.194~198 (2008)
- Dwyer JH, "Oxygenated carotenoid lutein and progression of early atherosclerosis: the Los Angeles atherosclerosis study", Circulation, 103, pp.2922~2927 (2001)
- Dwyer JH, "Progression of carotid intima-media thickness and plasma antioxidants: the Los Angeles Atherosclerosis Study", Arterioscler Thromb Vasc Biol, 24, pp.313~319 (2004)
- Sindhu ER, "Carotenoid lutein protects rats from paracetamol-, carbon tetrachloride- and ethanolinduced hepatic damage", J Pharm Pharmacol, 62, pp.1054~1060 (2010)
- Sindhu ER, "Antioxidant activity of carotenoid lutein in vitro and in vivo", Indian J Exp Biol, 48, pp.843~848 (2010)
- Jin XH, "Inhibitory effects of lutein on endotoxin-induced uveitis in Lewis rats", Invest Ophthalmol Vis Sci, 47, pp.2562~2568 (2006)
- 김지희, "The non-provitamin A carotenoid, lutein, inhibits NF-??B-dependent gene expression through redox-based regulation of the phosphatidylinositol 3-kinase", PTEN, Free Radical Biology and Medicine, pp.200809, 45(6)[DOI]
- Shanmugasundaram R, "Lutein supplementation alters inflammatory cytokine production and antioxidant status in F-line turkeys", Poult Sci, 90, pp.971~976 (2011)
- He G, "NF-kappaB and STAT3 - key players in liver inflammation and cancer", Cell Res, 21, pp.159~168 (2011)
- Luedde T, "NF-kappaB in the liver--linking injury, fibrosis and hepatocellular carcinoma", Nat Rev Gastroenterol Hepatol, 8, pp.108~118 (2011)
- Robinson SM, "Role of nuclear factor kappaB in liver health and disease", Clin Sci (Lond), 118, pp.691~705 (2010)
- Fernandez ML, "Guinea pigs as models for cholesterol and lipoprotein metabolism", J Nutr, 131, pp.10~20 (2001)
- Roy S, "Gender and hormonal status affect the hypolipidemic mechanisms of dietary soluble fiber in guinea pigs", J Nutr, 130, pp.600~607 (2000)
- Torres-Gonzalez M, "Carbohydrate restriction alters hepatic cholesterol metabolism in guinea pigs fed a hypercholesterolemic diet", J Nutr, 137, pp.2219~2223 (2007)
- Anstee QM, "Mouse models in non-alcoholic fatty liver disease and steatohepatitis research", Int J Exp Pathol, 87, pp.1~16 (2006)
- Kim JE, "A lutein-enriched diet prevents cholesterol accumulation and decreases oxidized LDL and inflammatory cytokines in the aorta of guinea pigs", J Nutr, 141, pp.1458~1463 (2011)
- Fernandez ML, "Regulation of cholesterol and lipoprotein metabolism in guinea pigs mediated by dietary fat quality and quantity", J Nutr, 121, pp.934~943 (1991)
- Clark RM, "Hypo- and hyperresponse to egg cholesterol predicts plasma lutein and betacarotene concentrations in men and women", J Nutr, 136, pp.601~607 (2006)
- Carr TP, "Enzymatic determination of triglyceride, free cholesterol, and total cholesterol in tissue lipid extracts", Clin Biochem, 26, pp.39~42 (1993)
- Schäffer MW, "Identification of lutein, a dietary antioxidant carotenoid in guinea pig tissues", Biochem Biophys Res Commun, 374, pp.378~381 (2008)
- Lin EC, "Dietary fat type and cholesterol quantity interact to affect cholesterol metabolism in guinea pigs", J Nutr, 122, pp.2019~2029 (1992)
- Shanmugasundaram R, "Dietary lutein and fish oil interact to alter atherosclerotic lesions in a Japanese quail model of atherosclerosis", J Anim Physiol Anim Nutr (Berl), 95, pp.762~770 (2011)
- Puri P, "A lipidomic analysis of nonalcoholic fatty liver disease", Hepatology, 46, pp.1081~1090 (2007)
- Sanyal AJ, "Nonalcoholic steatohepatitis: association of insulin resistance and mitochondrial abnormalities", Gastroenterology, 120, pp.1183~1192 (2001)
- Nair S, "Hepatic ATP reserve and efficiency of replenishing: comparison between obese and nonobese normal individuals", Am J Gastroenterol, 98, pp.466~470 (2003)
- McClain CJ, "Mechanisms of non-alcoholic steatohepatitis", Alcohol, 34, pp.67~79 (2004)
- Janero DR, "Malondialdehyde and thiobarbituric acid-reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury", Free Radic Biol Med, 9, pp.515~540 (1990)
- Young AJ, "Antioxidant and prooxidant properties of carotenoids", Arch Biochem Biophys, 385, pp.20~27 (2001)
- Ribaya-Mercado JD, "Lutein and zeaxanthin and their potential roles in disease prevention", J Am Coll Nutr, 23, pp.567~587 (2004)
- Debril MB, "The pleiotropic functions of peroxisome proliferator-activated receptor gamma", J Mol Med (Berl), 79, pp.30~47 (2001)
- Selvaraj RK, "Effects of dietary lutein and PUFA on PPAR and RXR isomer expression in chickens during an inflammatory response", Comp Biochem Physiol A Mol Integr Physiol, 157, pp.198~203 (2010)
- Mai J, "Effect of lutein on relieving oxidative stress in mice induced by D-galatose", Wei Sheng Yan Jiu, 39, pp.430~432 (2010)
- Ruhl CE, "Relation of elevated serum alanine aminotransferase activity with iron and antioxidant levels in the United States", Gastroenterology, 124, pp.1821~1829 (2003)
- Preethi KC, "Hepato and reno protective action of Calendula officinalis L. flower extract", Indian J Exp, 47, pp.163~168 (2009)
- Catala A, "Lipid peroxidation of membrane phospholipids in the vertebrate retina", Front Biosci (Schol Ed), 3, pp.52~60 (2011)
- Ham WT Jr, "Retinal sensitivity to damage from short wavelength light", Nature, 260, pp.153~155 (1976)
- Beatty S, "The role of oxidative stress in the pathogenesis of age-related macular degeneration", Surv Ophthalmol, 45, pp.115~134 (2000)