Vitamin A supplementation modifies the antioxidant system in rats
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BACKGROUND/OBJECTIVES: It has been shown that vitamin A supplementation has different effects on skeletal health andthe antioxidant system. Deficiency or excess of this vitamin can lead to health problems. Vitamin A can work as either anantioxidant or prooxidant depending on its concentration. The present study was conducted to investigate the effects of differentdoses of vitamin A supplementation on the antioxidant system in rats. MATERIALS/METHODS: Forty Spargue-Dawley male rats were divided into four groups according to the dose of vitamin Areceived: 0 (A0), 4,000 (A1), 8,000 (A2), and 20,000 (A3) IU retinyl palmitate/kg diet. After a feeding period of 4 wks, lipidperoxide levels, glutathione concentration, antioxidant enzyme activities, and vitamins A and E concentrations were measured. Histopathological changes were observed in rat liver tissue using an optical microscope and transmission electron microscope. RESULTS: Lipid peroxide levels in plasma were significantly decreased in the A1 and A2 groups compared to the A0 rats. Erythrocyte catalase and hepatic superoxide dismutase activities of the A2 group were significantly higher than those of theA0 group. Hepatic glutathione peroxidase activity was significantly lower in the A3 group compared to the other groups. Total glutathione concentrations were significantly higher in the A1 and A2 groups than in the A0 group. Histological examinationof liver tissue showed that excessive supplementation of vitamin A might lead to lipid droplet accumulation and nuclear membranedeformation. CONCLUSIONS: These results indicate that appropriate supplementation of vitamin A might have a beneficial effect on theantioxidant system in rats.
Abstract
BACKGROUND/OBJECTIVES: It has been shown that vitamin A supplementation has different effects on skeletal health andthe antioxidant system. Deficiency or excess of this vitamin can lead to health problems. Vitamin A can work as either anantioxidant or prooxidant depending on its concentration. The present study was conducted to investigate the effects of differentdoses of vitamin A supplementation on the antioxidant system in rats. MATERIALS/METHODS: Forty Spargue-Dawley male rats were divided into four groups according to the dose of vitamin Areceived: 0 (A0), 4,000 (A1), 8,000 (A2), and 20,000 (A3) IU retinyl palmitate/kg diet. After a feeding period of 4 wks, lipidperoxide levels, glutathione concentration, antioxidant enzyme activities, and vitamins A and E concentrations were measured. Histopathological changes were observed in rat liver tissue using an optical microscope and transmission electron microscope. RESULTS: Lipid peroxide levels in plasma were significantly decreased in the A1 and A2 groups compared to the A0 rats. Erythrocyte catalase and hepatic superoxide dismutase activities of the A2 group were significantly higher than those of theA0 group. Hepatic glutathione peroxidase activity was significantly lower in the A3 group compared to the other groups. Total glutathione concentrations were significantly higher in the A1 and A2 groups than in the A0 group. Histological examinationof liver tissue showed that excessive supplementation of vitamin A might lead to lipid droplet accumulation and nuclear membranedeformation. CONCLUSIONS: These results indicate that appropriate supplementation of vitamin A might have a beneficial effect on theantioxidant system in rats.
참고문헌 (45)
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