Optimal Fluence and Duration of Low-Level Laser Therapy for Efficient Wound Healing in Mice
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Background: Low-level laser (light) therapy is a promising technology that stimulates healing, relieves pain and inflammation, and restores function in injured body parts. However, few studies have compared the effects of lightemitting diodes of different fluence levels or different treatment durations. Objective: Here, we investigated the effects of various fluence levels and treatment durations on wound closure in mice. Methods: Full-thickness wounds were created on the dorsal skin using an 8-mm diameter punch, and the wounds were irradiated at 1, 4, or 40 J/cm2 for 5 consecutive days starting on day 1. To determine the optimal irradiation duration, wounds were irradiated at the most potent fluence of previous study for 5, 10, or 15 days. Photographic documentation, skin biopsies, and wound measurements were performed to compare the effects of different treatment parameters. Results: The most effective fluence level was 40 J/cm2 at day 5, as determined by monitoring wound closure. There were no statistically significant differences in wound healing with different durations. Conclusion: We have shown that repeated exposure to low levels of light significantly stimulates wound healing in mice and demonstrated more efficient wound closure with certain fluences of 830 nm irradiation.
Abstract
Background: Low-level laser (light) therapy is a promising technology that stimulates healing, relieves pain and inflammation, and restores function in injured body parts. However, few studies have compared the effects of lightemitting diodes of different fluence levels or different treatment durations. Objective: Here, we investigated the effects of various fluence levels and treatment durations on wound closure in mice. Methods: Full-thickness wounds were created on the dorsal skin using an 8-mm diameter punch, and the wounds were irradiated at 1, 4, or 40 J/cm2 for 5 consecutive days starting on day 1. To determine the optimal irradiation duration, wounds were irradiated at the most potent fluence of previous study for 5, 10, or 15 days. Photographic documentation, skin biopsies, and wound measurements were performed to compare the effects of different treatment parameters. Results: The most effective fluence level was 40 J/cm2 at day 5, as determined by monitoring wound closure. There were no statistically significant differences in wound healing with different durations. Conclusion: We have shown that repeated exposure to low levels of light significantly stimulates wound healing in mice and demonstrated more efficient wound closure with certain fluences of 830 nm irradiation.
참고문헌 (27)
- Surinchak JS, "Effects of low-level energy lasers on the healing of fullthickness skin defects", Lasers Surg Med, 2, pp.267~274 (1983)
- Andrade Fdo S, "Effects of low-level laser therapy on wound healing", Rev Col Bras Cir, 41, pp.129~133 (2014)
- Bashardoust Tajali S, "Effects of low power laser irradiation on bone healing in animals : a meta-analysis", J Orthop Surg Res, 5, pp.1~ (2010)
- Channual J, "Vascular effects of photodynamic and pulsed dye laser therapy protocols", Lasers Surg Med, 40, pp.644~650 (2008)
- Al-Watban FAH, "Laser photons and pharmacological treatments in wound healing", Laser Ther, 12, pp.3~11 (2000)
- Da Silva TS, "Estudo microscópio da lesão tecidual em pele de ratos Wistar, tratados com laser de baixa potência", Brazilian J Biosci, 8, pp.264~267 (2010)
- Maiya AG, "Photo-stimulatory effect of low energy helium-neon laser irradiation on excisional diabetic wound healing dynamics in Wistar rats", Indian J Dermatol, 54, pp.323~329 (2009)
- Stadler I, "830-nm irradiation increases the wound tensile strength in a diabetic murine model", Lasers Surg Med, 28, pp.220~226 (2001)
- Rathnakar B, "Photo-biomodulatory response of low-power laser irradiation on burn tissue repair in mice", Lasers Med Sci, 31, pp.1741~1750 (2016)
- Hegde VN, "Effect of laser dose and treatment schedule on excision wound healing in diabetic mice", Photochem Photobiol, 87, pp.1433~1441 (2011)
- Demidova-Rice TN, "Low-level light stimulates excisional wound healing in mice", Lasers Surg Med, 39, pp.706~715 (2007)
- Medrado AR, "Influence of low level laser therapy on wound healing and its biological action upon myofibroblasts", Lasers Surg Med, 32, pp.239~244 (2003)
- Van Breugel HH, "Power density and exposure time of He-Ne laser irradiation are more important than total energy dose in photo-biomodulation of human fibroblasts in vitro", Lasers Surg Med, 12, pp.528~537 (1992)
- Coombe AR, "The effects of low level laser irradiation on osteoblastic cells", Clin Orthod Res, 4, pp.3~14 (2001)
- Karu TI, "Exact action spectra for cellular responses relevant to phototherapy", Photomed Laser Surg, 23, pp.355~361 (2005)
- Greco M, "Increase in RNA and protein synthesis by mitochondria irradiated with helium-neon laser", Biochem Biophys Res Commun, 163, pp.1428~1434 (1989)
- Karu TI, "Photobiological modulation of cell attachment via cytochrome c oxidase", Photochem Photobiol Sci, 3, pp.211~216 (2004)
- Chiang B, "Enhancing skin wound healing by direct delivery of intracellular adenosine triphosphate", Am J Surg, 193, pp.213~218 (2007)
- Kreis TE, "Stress fiber sarcomeres of fibroblasts are contractile", Cell, 22(2 Pt 2), pp.555~561 (1980)
- Ehrlich HP, "Vanadate and the absence of myofibroblasts in wound contraction", Arch Surg, 134, pp.494~501 (1999)
- Uitto J, "Cytokine modulation of extracellular matrix gene expression : relevance to fibrotic skin diseases", J Dermatol Sci, 24(Suppl 1), pp.S60~S69 (2000)
- Wolfram D, "Hypertrophic scars and keloids--a review of their pathophysiology, risk factors, and therapeutic management", Dermatol Surg, 35, pp.171~181 (2009)
- Uitto J, "IL-6 signaling pathway in keloids : a target for pharmacologic intervention", J Invest Dermatol, 127, pp.6~8 (2007)
- Ghazizadeh M, "Functional implications of the IL-6 signaling pathway in keloid pathogenesis", J Invest Dermatol, 127, pp.98~105 (2007)
- Liu W, "TGF-beta : a fibrotic factor in wound scarring and a potential target for anti-scarring gene therapy", Curr Gene Ther, 4, pp.123~136 (2004)
- Lee SY, "A prospective, randomized, placebo-controlled, doubleblinded, and split-face clinical study on LED phototherapy for skin rejuvenation: clinical, profilometric, histologic, ultrastructural, and biochemical evaluations and comparison of three different treatment settings", J Photochem Photobiol B, 88, pp.51~67 (2007)
- Barolet D, "Prophylactic low-level light therapy for the treatment of hypertrophic scars and keloids : a case series", Lasers Surg Med, 42, pp.597~601 (2010)