Anti-inflammatory effect of lycopene in SW480 human colorectal cancer cells
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
BACKGROUND/OBJECTIVES: Although the antioxidative effects of lycopene are generally known, the molecular mechanisms underlying the anti-inflammatory properties of lycopene are not fully elucidated. This study aimed to examine the role and mechanism of lycopene as an inhibitor of inflammation. METHODS/MATERIALS: Lipopolysaccharide (LPS)-stimulated SW 480 human colorectal cancer cells were treated with 0, 10, 20, and 30 μM lycopene. The MTT assay was performed to determine the effects of lycopene on cell proliferation. Western blotting was performed to observe the expression of inflammation-related proteins, including nuclear factor-kappa B (NF-κB), inhibitor kappa B (IκB), mitogen-activated protein kinase (MAPK), extracellular signal-related kinase (ERK), c-jun NH2-terminal kinase (JNK), and p38 (p38 MAP kinase). Real-time polymerase chain reaction was performed to investigate the mRNA expression of tumor necrosis factor α (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2). Concentrations of nitric oxide (NO) and prostaglandin E2 (PGE2) were determined via enzyme-linked immunosorbent assays. RESULTS: In cells treated with lycopene and LPS, the mRNA expression of TNF-α, IL-1β, IL-6, iNOS, and COX-2 were decreased significantly in a dose-dependent manner (P < 0.05). The concentrations of PGE2 and NO decreased according to the lycopene concentration (P < 0.05). The protein expressions of NF-κB and JNK were decreased significantly according to lycopene concertation (P < 0.05). CONCLUSIONS: Lycopene restrains NF-κB and JNK activation, which causes inflammation, and suppresses the expression of TNF-α, IL-1β, IL-6, COX-2, and iNOS in SW480 human colorectal cancer cells.
Abstract
BACKGROUND/OBJECTIVES: Although the antioxidative effects of lycopene are generally known, the molecular mechanisms underlying the anti-inflammatory properties of lycopene are not fully elucidated. This study aimed to examine the role and mechanism of lycopene as an inhibitor of inflammation. METHODS/MATERIALS: Lipopolysaccharide (LPS)-stimulated SW 480 human colorectal cancer cells were treated with 0, 10, 20, and 30 μM lycopene. The MTT assay was performed to determine the effects of lycopene on cell proliferation. Western blotting was performed to observe the expression of inflammation-related proteins, including nuclear factor-kappa B (NF-κB), inhibitor kappa B (IκB), mitogen-activated protein kinase (MAPK), extracellular signal-related kinase (ERK), c-jun NH2-terminal kinase (JNK), and p38 (p38 MAP kinase). Real-time polymerase chain reaction was performed to investigate the mRNA expression of tumor necrosis factor α (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2). Concentrations of nitric oxide (NO) and prostaglandin E2 (PGE2) were determined via enzyme-linked immunosorbent assays. RESULTS: In cells treated with lycopene and LPS, the mRNA expression of TNF-α, IL-1β, IL-6, iNOS, and COX-2 were decreased significantly in a dose-dependent manner (P < 0.05). The concentrations of PGE2 and NO decreased according to the lycopene concentration (P < 0.05). The protein expressions of NF-κB and JNK were decreased significantly according to lycopene concertation (P < 0.05). CONCLUSIONS: Lycopene restrains NF-κB and JNK activation, which causes inflammation, and suppresses the expression of TNF-α, IL-1β, IL-6, COX-2, and iNOS in SW480 human colorectal cancer cells.
참고문헌 (38)
- "Cause of death" (2014)[URL]
- "Cancer registration statistics" (2013)[URL]
- 김동현, "대장암의 발병위험요인", Annals of Coloproctolgy, 25(5), pp.356~362 (2009), 대한대장항문학회
- Coussens LM, "Inflammation and cancer", Nature, 420, pp.860~867 (2002)
- Tuan J, "Dietary and lifestyle factors associated with colorectal cancer risk and interactions with microbiota: fiber, red or processed meat and alcoholic drinks", Gastrointest Tumors, 3, pp.17~24 (2016)
- Cho YA, "Dietary inflammatory index and risk of colorectal cancer: a case-control study in Kore", Nutrients, 8, pp.E469~ (2016)
- Youn SW, "Systemic inflammatory response as a prognostic factor in patients with cancer", J Korean Orient Oncol, 17, pp.1~7 (2012)
- Danese S, "Inflammatory bowel disease and intestinal cancer: a paradigm of the Yin-Yang interplay between inflammation and cancer", Oncogene, 29, pp.3313~3323 (2010)
- Holzapfel NP, "The potential role of lycopene for the prevention and therapy of prostate cancer: from molecular mechanisms to clinical evidence", Int J Mol Sci, 14, pp.14620~14646 (2013)
- Aree MOON, "Lycopene Inhibits Proliferation, Invasion and Migration of Human Breast Cancer Cells", Biomolecules & Therapeutics, 18(1), pp.92~98 (2010), 한국응용약물학회
- Giovannucci E., "Tomatoes, tomato-based products, lycopene, and cancer: review of the epidemiologic literature", J Natl Cancer Inst, 91, pp.317~331 (1999)
- Michaud DS, "Intake of specific carotenoids and risk of lung cancer in 2 prospective US cohorts", Am J Clin Nutr, 72, pp.990~997 (2000)
- Giovannucci E, "Intake of carotenoids and retinol in relation to risk of prostate cancer", J Natl Cancer Inst, 87, pp.1767~1776 (1995)
- -Gingerol이 인체 유방암세포인 MDA-MB-231의세포증식 억제에 미치는 영향, "Journal of Nutrition and Health", 2005, 656~662, pp.한국영양학회
- Lin D, "Penehyclidine hydrochloride preconditioning provides cardioprotection in a rat model of myocardial ischemia", reperfusion injury, 2015, pp.10, e0138051~
- Livak KJ, "Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T))method", Methods, 25, pp.402~408 (2001)
- Sang-Ho Lee, "Confirmation of Male Specific Fetal Free RNA in Maternal Plasma and Comparison of Accuracy on the Sex Determination using Real-time PCR Method in Korean Native Cattle", 한국수정란이식학회지, 28(4), pp.343~348 (2013), 한국수정란이식학회
- McCarthy MK, "Prostaglandin E2 production and T cell function in mouse adenovirus type 1 infection following allogeneic bone marrow transplantation", PLoS One, 10, pp.e0139235~ (2015)
- Kang HJ, "Anti-inflammatory effects of Hwang-Heuk-San, a traditional Korean herbal formulation, on lipopolysaccharide-stimulated murine macrophages", BMC Complement Altern Med, 15, pp.447~ (2015)
- Slattery ML, "Dietary influence on MAPKsignaling pathways and risk of colon and rectal cancer", Nutr Cancer, 65, pp.729~738 (2013)
- 이현숙, "인간의 대장암 HT-29 세포주에서 라이코펜이 Insulin-like Growth Factor-I Receptor Signaling Pathway에 미치는 영향", 한국식품영양과학회지, 32(3), pp.437~443 (2003), 한국식품영양과학회
- Huang RF, "Inhibition of colon cancer cell growth by nanoemulsion carrying gold nanoparticles and lycopene", Int J Nanomedicine, 10, pp.2823~2846 (2015)
- Tang FY, "Lycopene inhibits growth of human colon cancer cells via suppression of the Akt signaling pathway", Mol Nutr Food Res, 52, pp.646~654 (2008)
- Lin MC, "Cancer chemopreventive effects of lycopene: suppression of MMP-7 expression and cell invasion in human colon cancer cells", J Agric Food Chem, 59, pp.11304~11318 (2011)
- Triantafilou M, "The dynamics of LPS recognition:complex orchestration of multiple receptors", J Endotoxin Res, 11, pp.5~11 (2005)
- Hung CF, "Lycopene inhibits TNF-alpha-induced endothelial ICAM-1 expression and monocyte-endothelial adhesion", Eur J Pharmacol, 586, pp.275~282 (2008)
- Bessler H, "In vitro effect of lycopene on cytokine production by human peripheral blood mononuclear cells", Immunol Invest, 37, pp.183~190 (2008)
- Feng D, "Lycopene suppresses LPS-induced NO and IL-6 production by inhibiting the activation of ERK, p38MAPK, and NF-κB in macrophages", Inflamm Res, 59, pp.115~121 (2010)
- Rafi MM, "Lycopene inhibits LPS-induced proinflammatory mediator inducible nitric oxide synthase in mouse macrophage cells", J Food Sci, 72, pp.S069~S074 (2007)
- Bonizzi G, "The two NF-κB activation pathways and their role in innate and adaptive immunity", Trends Immunol, 25, pp.280~288 (2004)
- Reifen R, "Lycopene supplementation attenuates the inflammatory status of colitis in a rat model", Int J Vitam Nutr Res, 71, pp.347~351 (2001)
- Riso P, "Effect of a tomato-based drink on markers of inflammation, immunomodulation, and oxidative stress", J Agric Food Chem, 54, pp.2563~2566 (2006)
- Vrieling A, "Lycopene supplementation elevates circulating insulin-like growth factor binding protein-1 and -2concentrations in persons at greater risk of colorectal cancer", Am J Clin Nutr, 86, pp.1456~1462 (2007)
- Levy J, "Lycopene is a more potent inhibitor of human cancer cell proliferation than either alpha-carotene or beta-carotene", Nutr Cancer, 24, pp.257~266 (1995)
- Zhang N, "NF-kappaB and not the MAPK signaling pathway regulates GADD45beta expression during acute inflammation", J Biol Chem, 280, pp.21400~21408 (2005)
- Palozza P, "Tomato lycopene and inflammatory cascade: basic interactions and clinical implications", Curr Med Chem, 17, pp.2547~2563 (2010)
- Kim GY, "Lycopene suppresses the lipopolysaccharide-induced phenotypic and functional maturation of murine dendritic cells through inhibition of mitogen-activated protein kinases and nuclear factorkappaB", Immunology, 113, pp.203~211 (2004)
- Hardwick JC, "NF-kappaB, p38 MAPK and JNK are highly expressed and active in the stroma of human colonic adenomatous polyps", Oncogene, 20, pp.819~827 (2001)