COX-2 inhibitory effect of luteolin through the regulation of NF-κB and ERK in phorbol ester activated human lung epithelial cells
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The inhibitory effect of luteolin on cyclooxygenase (COX)-2 expression was investigated in 12-O-tetradecanoyl phorbol-13-acetate (TPA), a prototype tumor promoter, activated human lung epithelial A549 cells. Luteolin mitigated TPA-stimulated COX-2 expression in a dose-dependent manner without any cytotoxicity. Since nuclear factor (NF)-κB plays a critical role in inflammatory casdades, activated status of NF-κB was estimated through the phosphorylation of p65, one subunit of NF-κB by western blot analysis. Phosphorylation of p65 was dose-dependently inhibited by luteolin treatment. Mitogen-activated protein kinases (MAPKs) are also important signaling molecules for COX-2 regulation. Luteolin treatment inhibited TPA-induced extracellular-signal regulated protein kinase (ERK) phosphorylation that is crucially related to inflammatory cascades regulation. These results suggest that luteolin attenuates TPA-induced COX-2 expression by blocking NF-κB activation through suppressed ERK phosphorylation in A549 cells.
Abstract
The inhibitory effect of luteolin on cyclooxygenase (COX)-2 expression was investigated in 12-O-tetradecanoyl phorbol-13-acetate (TPA), a prototype tumor promoter, activated human lung epithelial A549 cells. Luteolin mitigated TPA-stimulated COX-2 expression in a dose-dependent manner without any cytotoxicity. Since nuclear factor (NF)-κB plays a critical role in inflammatory casdades, activated status of NF-κB was estimated through the phosphorylation of p65, one subunit of NF-κB by western blot analysis. Phosphorylation of p65 was dose-dependently inhibited by luteolin treatment. Mitogen-activated protein kinases (MAPKs) are also important signaling molecules for COX-2 regulation. Luteolin treatment inhibited TPA-induced extracellular-signal regulated protein kinase (ERK) phosphorylation that is crucially related to inflammatory cascades regulation. These results suggest that luteolin attenuates TPA-induced COX-2 expression by blocking NF-κB activation through suppressed ERK phosphorylation in A549 cells.
참고문헌 (24)
- C. D. Munhoz, , Braz. J. Med. Biol. Res, 41, pp.1037~1046 (2008)
- H. Kolb, , Immunol. Today, 13, pp.157~160 (1992)
- S. A. Park, , Ann. N. Y. Acad. Sci, 1095, pp.545~553 (2007)
- H. K. Na, , Mol. Nutr. Food Res, 50, pp.152~159 (2006)
- J. H. Kim, , Chem. Biol. Interact, 171, pp.133~141 (2008)
- T. Kawamori, , Cancer Res, 58, pp.409~412 (1998)
- F, Yang, , J. Nutr, 128, pp.2334~2340 (1998)
- W. C. Sha, , J. Exp. Med, 187, pp.143~146 (1998)
- J. L. Devalia, , Respir. Med, 87, pp.405~408 (1993)
- J. A. Mitchell, , Br. J. Pharmacol, 113, pp.1008~1014 (1994)
- H. Inoue, , J. Biol. Chem, 270, pp.24965~24971 (1995)
- R. G. Allen, , Free Radic. Biol. Med, 28, pp.463~499 (2000)
- D. Gius, , Toxicol. Lett, 106, pp.93~106 (1999)
- S. Jang, , Proc. Natl. Acad. Sci. U. S. A, 105, pp.7534~7539 (2008)
- G. K. Harris, , J. Nutr, 136, pp.1517~1521 (2006)
- G. Seelinger, , Planta Med, 74, pp.1667~1677 (2008)
- 박충무, "Luteolin Inhibits Inflammatory Responses by Downregulating the JNK, NF-κB, and AP-1 Pathways in TNF-α Activated HepG2 Cells", Food Science and Biotechnology, 21(1), pp.279~283 (2012), 한국식품과학회
- Y. Cao, , J. Cell. Physiol, 190, pp.279~286 (2002)
- Y. J. Surh, , Food Chem. Toxicol, 40, pp.1091~1097 (2002)
- Y. J. Surh, , Nat. Rev. Cancer, 3, pp.768~780 (2003)
- V. Dixit, , Cell, 111, pp.615~619 (2002)
- B. B. Aggarwal, , Biochem. Pharmacol, 71, pp.1397~1421 (2006)
- S. Fujioka, , Mol. Cell Biol, 24, pp.7806~7819 (2004)
- G. L. Johnson, , Science, 298, pp.1911~1912 (2002)