Use of Speckle Tracking Echocardiography to Detect Induced Regional Strain Changes in the Murine Myocardium by Acoustic Radiation Force
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BACKGROUND: It is difficult to simulate the abnormal myocardial strain patterns caused by ischemic coronary artery disease (CAD) which are a precursor to heart failure (HF) within an animal model. Simulation of these strain changes could contribute to better understanding of the early formative stages of HF. This is especially important in investigating the poorly understood pathogenesis of heart failure with preserved ejection fraction (HFpEF). Here, we discuss delivery of high intensity focused ultrasound (HIFU) in a murine model to alter left ventricular (LV) regional longitudinal strain (RLS), and use of speckle tracking echocardiography to detect these changes. METHODS: HIFU pulses (pressure amplitude 1.7 MPa) were generated by amplifying a sinusoidal waveform from a function generator into a piezoelectric transducer. These pulses were then directed extracorporeally towards the anterior LV surface of C57BI6 mice during three time periods (early, mid, and late diastole). Speckle tracking echocardiography was then used to quantify changes in RLS within six segments of the LV. RESULTS: We observed an increase in LV RLS with acoustic augmentation during all three time periods. This augmentation was most prominent near the anterior apical region in early diastole and near the posterior basilar region during late diastole. CONCLUSIONS: Our findings demonstrate the application of HIFU to non-invasively induce changes in RLS within a murine model. Our results also reflect the capability of speckle tracking echocardiography to analyze and quantify these changes. These findings represent the first demonstration of ultrasound-induced augmentation in LV RLS within a small animal model.
Abstract
BACKGROUND: It is difficult to simulate the abnormal myocardial strain patterns caused by ischemic coronary artery disease (CAD) which are a precursor to heart failure (HF) within an animal model. Simulation of these strain changes could contribute to better understanding of the early formative stages of HF. This is especially important in investigating the poorly understood pathogenesis of heart failure with preserved ejection fraction (HFpEF). Here, we discuss delivery of high intensity focused ultrasound (HIFU) in a murine model to alter left ventricular (LV) regional longitudinal strain (RLS), and use of speckle tracking echocardiography to detect these changes. METHODS: HIFU pulses (pressure amplitude 1.7 MPa) were generated by amplifying a sinusoidal waveform from a function generator into a piezoelectric transducer. These pulses were then directed extracorporeally towards the anterior LV surface of C57BI6 mice during three time periods (early, mid, and late diastole). Speckle tracking echocardiography was then used to quantify changes in RLS within six segments of the LV. RESULTS: We observed an increase in LV RLS with acoustic augmentation during all three time periods. This augmentation was most prominent near the anterior apical region in early diastole and near the posterior basilar region during late diastole. CONCLUSIONS: Our findings demonstrate the application of HIFU to non-invasively induce changes in RLS within a murine model. Our results also reflect the capability of speckle tracking echocardiography to analyze and quantify these changes. These findings represent the first demonstration of ultrasound-induced augmentation in LV RLS within a small animal model.
참고문헌 (29)
- D'Elia N, "Association between myocardial mechanics and ischemic LV remodeling", JACC Cardiovasc Imaging, 8, pp.1430~1443 (2015)
- Liszka J, "Two-dimensional speckle-tracking echocardiography assessment of left ventricular remodeling in patients after myocardial infarction and primary reperfusion", Arch Med Sci, 10, pp.1091~1100 (2014)
- Moaref A, "Evaluation of global and regional strain in patients with acute coronary syndrome without previous myocardial infarction", Int Cardiovasc Res J, 10, pp.6~11 (2016)
- Ernande L, "Longitudinal myocardial strain alteration is associated with left ventricular remodeling in asymptomatic patients with type 2 diabetes mellitus", J Am Soc Echocardiogr, 27, pp.479~488 (2014)
- Tsai WC, "Diagnostic value of segmental longitudinal strain by automated function imaging in coronary artery disease without left ventricular dysfunction", J Am Soc Echocardiogr, 23, pp.1183~1189 (2010)
- D'Andrea A, "Global longitudinal speckle-tracking strain is predictive of left ventricular remodeling after coronary angioplasty in patients with recent non-ST elevation myocardial infarction", Int J Cardiol, 153, pp.185~191 (2011)
- Zaliaduonyte-Peksiene D, "Does longitudinal strain predict left ventricular remodeling after myocardial infarction?", Echocardiography, 29, pp.419~427 (2012)
- Riehle C, "Small animal models of heart failure", Cardiovasc Res, 115, pp.1838~1849 (2019)
- Gao E, "A novel and efficient model of coronary artery ligation and myocardial infarction in the mouse", Circ Res, 107, pp.1445~1453 (2010)
- Valero-Muñoz M, "Murine models of heart failure with preserved ejection fraction : a"Fishing Expedition"", JACC Basic Transl Sci, 2, pp.770~789 (2017)
- Torr GR, "The acoustic radiation force", Am J Phys, 52, pp.402~408 (1984)
- Day AC, "Laser-assisted cataract surgery versus standard ultrasound phacoemulsification cataract surgery", Cochrane Database Syst Rev, 7 (2016)
- Marquet F, "Non-invasive cardiac pacing with image-guided focused ultrasound", Sci Rep, 6, pp.36534~ (2016)
- Bohs LN, "Speckle tracking for multi-dimensional flow estimation", Ultrasonics, 38, pp.369~375 (2000)
- Hiesinger W, "Myocardial tissue elastic properties determined by atomic force microscopy after stromal cell-derived factor 1α angiogenic therapy for acute myocardial infarction in a murine model", J Thorac Cardiovasc Surg, 143, pp.962~966 (2012)
- Couade M, "In vivo quantitative mapping of myocardial stiffening and transmural anisotropy during the cardiac cycle", IEEE Trans Med Imaging, 30, pp.295~305 (2011)
- "IACUC guidelines and procedures"[URL]
- Sharma K, "Heart failure with preserved ejection fraction: mechanisms, clinical features, and therapies", Circ Res, 115, pp.79~96 (2014)
- Kitzman DW, "Heart failure with preserved ejection fraction : a heterogenous disorder with multifactorial pathophysiology", J Am Coll Cardiol, 63, pp.457~459 (2014)
- Borlaug BA, "The pathophysiology of heart failure with preserved ejection fraction", Nat Rev Cardiol, 11, pp.507~515 (2014)
- Andrew Kohut, "Comprehensive Echocardiographic Assessment of the Right Ventricle in Murine Models", Journal of Cardiovascular Imaging, 24(3), pp.229~238 (2016), 한국심초음파학회
- Goswami SK, "Expression and activation of BKCa channels in mice protects against ischemia-reperfusion injury of isolated hearts by modulating mitochondrial function", Front Cardiovasc Med, 5, pp.194~ (2019)
- Patel NH, "Inhibition of BKCa negatively alters cardiovascular function", Physiol Rep, 6, pp.e13748~ (2018)
- Cook MJ, "The anatomy of the laboratory mouse", pp.89~93 (1965), Academic Press
- Hynynen K, "The threshold for thermally significant cavitation in dog's thigh muscle in vivo", Ultrasound Med Biol, 17, pp.157~169 (1991)
- Bigelow TA, "The thermal index: its strengths, weaknesses, and proposed improvements", J Ultrasound Med, 30, pp.714~734 (2011)
- Palmeri ML, "On the thermal effects associated with radiation force imaging of soft tissue", IEEE Trans Ultrason Ferroelectr Freq Control, 51, pp.551~565 (2004)
- Lee LA, "High intensity focused ultrasound effect on cardiac tissues : potential for clinical application", Echocardiography, 17, pp.563~566 (2000)
- Swaminathan A, "Feasibility of noninvasive 3 T MRI-guided myocardial ablation with high intensity focused ultrasound", J Cardiovasc Magn Reson, 11(Suppl 1), pp.O86~ (2009)