Role of heat shock protein 70 in regulation of anti-inflammatory response to curcumin in 3T3-L1 adipocytes
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BACKGROUND/OBJECTIVES: Curcumin is a well-known phytochemical with anti- inflammator y effects. Heat shock protein (HSP) 70, an intracellular chaperone, inhibits proinflammator y signaling activation. Although curcumin has been shown to induce HSP70 expression in various cell types, whether HSP70 mediates the anti-inflammator y effects of curcumin in mature adipocytes remains unclear. MATERIALS/METHODS: To assess the role of HSP70 in regulating the anti-inflammator y response to curcumin in adipocytes, fully differentiated 3T3-L1 adipocytes were treated with curcumin, lipopolysaccharide (LPS), and/or the HSP70 inhibitor pifithrin-μ (PFT-μ). The expression levels of HSP70 and proinflammator y cytokines were then measured. RESULTS: Curcumin upregulated HSP70 expression at both protein and mRNA levels and attenuated LPS-induced Il6, Ptx3, and Ccl2 mRNA upregulation. PFT-μ tended to exacerbate the LPS-induced upregulation of Il6, Ptx3, Ccl2, and Tnfa mRNA expression. However, on curcumin pretreatment, the tendency of PFT-μ to upregulate LPS-induced proinflammator y cytokine expression decreased or disappeared. CONCLUSION: These results indicate that HSP70 is involved in the regulation of inflammator y responses but may not be crucial for the anti-inflammator y effects of curcumin in 3T3-L1 adipocytes.
Abstract
BACKGROUND/OBJECTIVES: Curcumin is a well-known phytochemical with anti- inflammator y effects. Heat shock protein (HSP) 70, an intracellular chaperone, inhibits proinflammator y signaling activation. Although curcumin has been shown to induce HSP70 expression in various cell types, whether HSP70 mediates the anti-inflammator y effects of curcumin in mature adipocytes remains unclear. MATERIALS/METHODS: To assess the role of HSP70 in regulating the anti-inflammator y response to curcumin in adipocytes, fully differentiated 3T3-L1 adipocytes were treated with curcumin, lipopolysaccharide (LPS), and/or the HSP70 inhibitor pifithrin-μ (PFT-μ). The expression levels of HSP70 and proinflammator y cytokines were then measured. RESULTS: Curcumin upregulated HSP70 expression at both protein and mRNA levels and attenuated LPS-induced Il6, Ptx3, and Ccl2 mRNA upregulation. PFT-μ tended to exacerbate the LPS-induced upregulation of Il6, Ptx3, Ccl2, and Tnfa mRNA expression. However, on curcumin pretreatment, the tendency of PFT-μ to upregulate LPS-induced proinflammator y cytokine expression decreased or disappeared. CONCLUSION: These results indicate that HSP70 is involved in the regulation of inflammator y responses but may not be crucial for the anti-inflammator y effects of curcumin in 3T3-L1 adipocytes.
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