Tangeretin Improves Glucose Uptake in a Coculture of Hypertrophic Adipocytes and Macrophages by Attenuating Inflammatory Changes
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Obesity is characterized by a state of chronic low-grade inflammation and insulin resistance, which are aggravated by the interaction between hypertrophic adipocytes and macrophages. In this study, we investigated the effects of tangeretin on inflammatory changes and glucose uptake in a coculture of hypertrophic adipocytes and macrophages. Tangeretin decreased nitric oxide production and the expression of interleukin (IL)-6, IL-1β, tumor necrosis factor-α, inducible nitric oxide synthase, and cyclooxygenase-2 in a coculture of 3T3-L1 adipocytes and RAW 264.7 cells. Tangeretin also increased glucose uptake in the coculture system, but did not affect the phosphorylation of insulin receptor substrate (IRS) and Akt. These results suggest that tangeretin improves insulin resistance by attenuating obesity-induced inflammation in adipose tissue.
Abstract
Obesity is characterized by a state of chronic low-grade inflammation and insulin resistance, which are aggravated by the interaction between hypertrophic adipocytes and macrophages. In this study, we investigated the effects of tangeretin on inflammatory changes and glucose uptake in a coculture of hypertrophic adipocytes and macrophages. Tangeretin decreased nitric oxide production and the expression of interleukin (IL)-6, IL-1β, tumor necrosis factor-α, inducible nitric oxide synthase, and cyclooxygenase-2 in a coculture of 3T3-L1 adipocytes and RAW 264.7 cells. Tangeretin also increased glucose uptake in the coculture system, but did not affect the phosphorylation of insulin receptor substrate (IRS) and Akt. These results suggest that tangeretin improves insulin resistance by attenuating obesity-induced inflammation in adipose tissue.
참고문헌 (26)
- Bijland S, "Role of AMP-activated protein kinase in adipose tissue metabolism and inflammation", Clin Sci, 124, pp.491~507 (2013)
- Chen KH, "Tangeretin suppresses IL-1beta-induced cyclooxygenase (COX)-2 expression through inhibition of p38 MAPK, JNK, and AKT activation in human lung carcinoma cells", Biochem Pharmacol, 73, pp.215~227 (2007)
- Choi SY, "Correlation between flavonoid content and the NO production inhibitory activity of peel extract from various citrus fruits", Biol Pharm Bull, 30, pp.772~778 (2007)
- Dandona P, "Inflammation:the link between insulin resistance, obesity and diabetes", Trends Immunol, 25, pp.4~7 (2004)
- Fernández-Real JM, "Insulin resistance and chronic cardiovascular inflammatory syndrome", Endocr Rev, 24, pp.278~301 (2003)
- Hotamisligil GS, "Increased adipose tissue expression of tumor necrosis factor-alpha in human obesity and insulin resistance", J Clin Invest, 95, pp.2409~2415 (1995)
- Itoh M, "Adipose tissue remodeling as homeostatic inflammation", Int J Inflam, 2011, pp.720926~ (2011)
- 전용필, "A Testa Extract of Black Soybean (Glycine max (L.) Merr.) suppresses Adipogenic Activity of Adipose-derived Stem Cells", 발생과 생식, 19(4), pp.235~242 (2015), 한국발생생물학회
- Kahn BB, "Obesity and insulin resistance", J Clin Invest, 106, pp.473~481 (2000)
- Kang SI, "Immature Citrus sunki peel extract exhibit antiobesity effects by β-oxidation and lipolysis in high-fat diet-induced obese mice", Biol Pharm Bull, 35, pp.223~316 (2012)
- Kanzaki M, "Insulin receptor signals regulating GLUT4 translocation and actin dynamics", Endocr J, 53, pp.267~293 (2006)
- Kim MS, "Tangeretin stimulates glucose uptake via regulation of AMPK signaling pathways in C2C12 myotubes and improves glucose tolerance in high-fat diet-induced obese mice", Mol Cell Endocrinol, 58, pp.127~234 (2012)
- Luca CD, "Inflammation and Insulin Resistance", FEBS Lett, 582, pp.97~105 (2008)
- Morley KL, "Tangeretin and nobiletin induce G1 cell cycle arrest but not apoptosis in human breast and colon cancer cells", Cancer Lett, 251, pp.168~178 (2007)
- Nonogaki K, "Interleukin-6stimulates hepatic triglyceride secretion in rats", Endocrinology, 136, pp.2143~2149 (1995)
- Onda K, "Polymethoxyflavonoids tangeretin and nobiletin increase glucose uptake in murine adipocytes", Phytother Res, 27, pp.312~316 (2013)
- Perreault M, "Targeted disruption of inducible nitric oxide synthase protects against obesitylinked insulin resistance in muscle", Nat Med, 7, pp.1138~1143 (2001)
- 김세재, "Resveratrol suppresses inflammatory responses and improves glucose uptake in adipocytes interacted with macrophages", Genes & Genomics, 38(2), pp.137~143 (2016), 한국유전학회[DOI]
- Spiegelman BM, "Adipogenesis and obesity:rounding out the big picture", Cell, 87, pp.377~389 (1996)
- Suganami T, "A paracrine loop between adipocytes and macrophages aggravates inflammatory changes: role of free fatty acids and tumor necrosis factor alpha", Arterioscler Thromb Vasc Biol, 25, pp.2062~2068 (2005)
- Sundaram R, "Effect of tangeretin, a polymethoxylated flavone on glucose metabolism in streptozotocin-induced diabetic rats", Phytomedicine, 21, pp.793~799 (2014)
- Wang P, "The secretory function of adipocytes in the physiology of white adipose tissue", J Cell Physiol, 216, pp.3~13 (2008)
- Weisberg SP, "Obesity is associated with macrophage accumulation in adipose tissue", J Clin Invest, 112, pp.1796~1808 (2003)
- Xu H, "Chronic inflammation in fat plays a crucial role in the development of obesity-related insulin resistance", J Clin Invest, 112, pp.1821~1830 (2003)
- Zhang HH, "Tumor necrosis factor-alpha stimulates lipolysis in differentiated human adipocytes through activation of extracellular signal-related kinase and elevation of intracellular cAMP", Diabetes, 51, pp.2929~2935 (2002)
- Zygmunt K, "Naringenin, a citrus flavonoid, increases muscle cell glucose uptake via AMPK", Biochem Biophys Res Commun, 398, pp.178~183 (2010)