Anti-proliferative and angio-suppressive effect of Stoechospermum marginatum (C. Agardh) Kutzing extract using various experimental models
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BACKGROUND/OBJECTIVES: Abundant consumption of seaweeds in the diet is epidemiologically linked to the reduction inrisk of developing cancer. In larger cases, however, identification of particular seaweeds that are accountable for these effectsis still lacking, hindering the recognition of competent dietary-based chemo preventive approaches. The aim of this researchwas to establish the antiproliferative potency and angiosuppressive mode of action of Stoechospermum marginatum seaweedmethanolic extract using various experimental models. MATERIALS/METHODS: Among the 15 seaweeds screened for antiproliferative activity against Ehrlich ascites tumor (EAT) cellline, Stoechospermum marginatum extract (SME) was found to be the most promising. Therefore, it was further investigatedfor its anti-proliferative activity in-vitro against choriocarcinoma (BeWo) and non-transformed Human embryonic kidney (HEK293) cells, and for its anti-migratory/tube formation activity against HUVEC cells in-vitro. Subsequently, the angiosuppressiveactivity of S. marginatum was established by inhibition of angiogenesis in in-vivo (peritoneal angiogenesis and chorioallantoicmembrane assay) and ex-vivo (rat cornea assay) models. RESULTS: Most brown seaweed extracts inhibited the proliferation of EAT cells, while green and red seaweed extracts weremuch less effective. According to the results, SME selectively inhibited proliferation of BeWo cells in-vitro in a dose-dependentmanner, but had a lesser effect on HEK 293 cells. SME also suppressed the migration and tube formation of HUVEC cellsin-vitro. In addition, SME was able to suppress VEGF-induced angiogenesis in the chorio allantoic membrane, rat cornea, andtumor induced angiogenesis in the peritoneum of EAT bearing mice. A decrease in the microvessel density count and CD31antigen staining of treated mice peritoneum provided further evidence of its angiosuppressive activity. CONCLUSIONS: Altogether, the data underline that VEGF mediated angiogenesis is the target for the angiosuppressive actionof SME and could potentially be useful in cancer prevention or treatment involving stimulated angiogenesis.
Abstract
BACKGROUND/OBJECTIVES: Abundant consumption of seaweeds in the diet is epidemiologically linked to the reduction inrisk of developing cancer. In larger cases, however, identification of particular seaweeds that are accountable for these effectsis still lacking, hindering the recognition of competent dietary-based chemo preventive approaches. The aim of this researchwas to establish the antiproliferative potency and angiosuppressive mode of action of Stoechospermum marginatum seaweedmethanolic extract using various experimental models. MATERIALS/METHODS: Among the 15 seaweeds screened for antiproliferative activity against Ehrlich ascites tumor (EAT) cellline, Stoechospermum marginatum extract (SME) was found to be the most promising. Therefore, it was further investigatedfor its anti-proliferative activity in-vitro against choriocarcinoma (BeWo) and non-transformed Human embryonic kidney (HEK293) cells, and for its anti-migratory/tube formation activity against HUVEC cells in-vitro. Subsequently, the angiosuppressiveactivity of S. marginatum was established by inhibition of angiogenesis in in-vivo (peritoneal angiogenesis and chorioallantoicmembrane assay) and ex-vivo (rat cornea assay) models. RESULTS: Most brown seaweed extracts inhibited the proliferation of EAT cells, while green and red seaweed extracts weremuch less effective. According to the results, SME selectively inhibited proliferation of BeWo cells in-vitro in a dose-dependentmanner, but had a lesser effect on HEK 293 cells. SME also suppressed the migration and tube formation of HUVEC cellsin-vitro. In addition, SME was able to suppress VEGF-induced angiogenesis in the chorio allantoic membrane, rat cornea, andtumor induced angiogenesis in the peritoneum of EAT bearing mice. A decrease in the microvessel density count and CD31antigen staining of treated mice peritoneum provided further evidence of its angiosuppressive activity. CONCLUSIONS: Altogether, the data underline that VEGF mediated angiogenesis is the target for the angiosuppressive actionof SME and could potentially be useful in cancer prevention or treatment involving stimulated angiogenesis.
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