유색칼라 기내 미세번식에 미치는 식물생장조절물질의 영향
Effect of Plant Growth Regulators on in vitro Micropropagation of Colored Calla Lily(Zantedeschia spp.)
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유 색 칼 라 세 품 종 (Sunlight, Chiante, Pink Persuation)의 기내 급속증식체계를 확립코자 정단분열조직을 배양함에 있어 callus, 신초 및 뿌리 분화에 미치는 식물생장조절물질의 효과와 sucrose가 기내괴경 형성 및 비대에 미치는 효과를 조사하였다. 식물체는 캘러스를 통하거나 또는 치상체에서 직접 분화되는 두 경로로 형성되었으며 2.0 mg/L BA 단용처리에서 Sunlight 품종은 53.3% 캘러스를, Chiante 품종은 56.7% 직접 신초를 형성하였다. 2.0-3.0 mg/L BA 단용 처리는 품종별로 식물체 재분화 빈도가 20-70% 로 차이가 있었으나 세 품종 모두 3.0 mg/L BA단용 처리에서 신초 분화가 양호하였다. 다아체 형성에 미치는 cytokinin의 효과는 Sunlight 품종은 1.0 mg/L 2ip 처리로 16개체를, Chiante 품종은 5.0 mg/L BA를 처리로 14개체를, Pink Persuasion 품종은 1.0mg/L BA 처리로 12개체를 형성하였다. NAA는 세 품종 뿌리분화에 효과가 없었으며 Sunlight 와 Chiante 품종은 1.0 mg/L IAA에서, Pink Persuasion 품종은 2.0 mg/L IBA가 효과적이었다. 괴경형성에 미치는 sucrose는 Sunlight 품종은 90 g/L, Chiante 및 Pink Persuasion 품종은 70 g/L 가 괴경형성 및 비대에 효과적이었다.
Abstract
To establish rapid micropropagation through organogenesis from apices-derived callus ordirect adventitious shoot of three calla lily cultivars(Zantedeschiaspp. cv. Sunlight, cv.Chiante, cv. Pink Persuation) were cultured on Murashige and Skoog mediumsupplemented with different plant growth regulators. The formation rate of callus,organogenesis and in vitrotuber production among the three cultivars were tested. Calluswas obtained from cvs. Sunlight, Chiante and Pink Persuasion; the best cultivar wasSunlight. Sunlight induced 53.3% callus and Chiante had the highest rate of 56.7% directshoot regeneration on medium with 2.0 mg/L BA. Regeneration frequencies ranged from 20to 70 % on medium with 2.0 - 3.0 mg/L BA. The highest percentage of regeneration and thegreatest number of shoots were obtained on medium containing 3.0 mg/L BA in threecultivars. Cytokinins induced multiple shoot formation; 1.0 mg/L of 2ip, 5.0 mg/L of BA,and 1.0 m/L of BA induced 16, 14 and 12 multiple shoots in cvs. Sunlight, Chiante and PinkPersuasion, respectivly. 1.0 mg/L of IAA enhanced root growth in cvs. Sunlight and Chiantewhile cv. Pink Persuasion exhibited enhanced root growth at 2.0 mg/L of IBA. NAA,however, induced no change in root growth. The addition of 90 g/L sucrose enhanced in vitrotuber formation and following tuber expansion in cv. Sunlight, while 70 g/L of sucrose waseffective in cvs. Chiante and Pink Persuasion.
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