범부채 (Belamcanda chinensis)의 식물체 재분화에 미치는 식물생장조절제의 영향
Effect of plant growth regulators on plant regeneration from the Belamcanda chinensis
0
조회
0
다운로드
0
인용
0
참고문헌
초록
범부채 (Belamcanda chinensis)의 기내 식물체 재분화 체계를 확립하기 위하여, 생장조절물질이 자엽, 뿌리줄기,뿌리 절편체로부터 캘러스 유도와 식물체 재분화에 미치는 영향을 조사하였다. 조직별 캘러스 유도율은 자엽절편체 보다 뿌리절편체에서 더 왕성하게 관찰되었으며,3.0 mg/L 2,4-D의 단독처리와 1.0 mg/L 2,4-D와 3.0 mg/L BA의 혼합처리에서 캘러스가 가장 효과적으로 유도되었다. 또한, 3.0 mg/L 2,4-D를 단독처리 한 배지에서 유도된캘러스를 치상하여 4주간 배양하였을 때 가장 많은 신초가 유도되었으며, BA를 혼합 처리한 배지에서는 다발성신초가 유도되었고 신초의 생장이 양호하였다. 2,4-D와BA를 혼합처리 한 배지로부터 유도된 신초를 생장조절물질을 첨가하지 않은 MS 배지로 옮겨 주었을 때 보다더 정상적이며 성장이 양호한 뿌리의 분화가 관찰되었다. 재분화 식물체는 순화처리를 위하여 당과 식물생장조절물질이 첨가되지 않은 MS배지로 계대배양 한 후 인공토양의 화분으로 옮겨 이식하여 관찰한 결과 100%의생존률을 보였으며 정상적인 재분화 식물체를 대량으로획득하였다.
Abstract
To establish the optimum conditions of in vitro plant regeneration, the leaf, rhizome, and root explants of Belamcanda chinensis were cultured on the MS medium supplemented with different concentration of 2,4-D and BA. The callus induction was more effective in the root explants than the leaf and rhizome explants, and was the best on MS medium containing 3.0 mg/L 2,4-D or 1.0mg/L 2,4-D and 3.0 mg/L BA. The highest numbers of shoots were regenerated when callus were cultured on MS medium containing 3.0 mg/L 2,4-D for 4 weeks. However, the multiple shoots were induced on MS medium supplemented with the combination of 2,4-D and BA. The normal root formation from shoot was effective on the MS medium lacking any plant growth regulators. For acclimatization, the regenerated plantlets were cultured on MS medium without sucrose and plant growth regulators for 2 weeks, and then transferred to the pot.
참고문헌 (23)
- Boltenkov EV, "Effect of phytohormones on plant regeneration in callus culture of Iris ensata Thunb", Biol Bull, 34, pp.446~450 (2007)
- Hong J, "Lead compounds for antiinflammatory drugs isolated from the plants of the traditional oriental medicine in Korea", Inflamm Allergy Drug Targets, 7, pp.195~202 (2008)
- Hussey G, "In vitro release of axillary shoots from apical dominance in monocotyledonous plantlets", Ann Bot, 40, pp.1323~1325 (1976)
- Jung SH, "Anti-angiogenic and anti-tumor activities of isoflavonoids from the rhizomes of Belamcanda chinensis", Planta Med, 69, pp.617~622 (2003)
- Jung SH, "Antioxidant activities of isoflavones from the rhizomes of Belamcanda chinensis on carbon tetrachloride-induced hepatic injury in rats", Arch Pharm Res, 27, pp.184~188 (2004)
- Kang MS, "Effects of culture conditions on adventitious bud formation from callus of Gladious ‘Topaz’", J Kor Soc Hort Sci, 39, pp.338~342 (1998)
- 김효진, "섬시호(Bupleurum latissimum Nakai)의 조직배양을 통한 대량생산", 한국자원식물학회지, 20(4), pp.367~374 (2007), 한국자원식물학회
- Koh JC, "Callus culture and plant regeneration from shoot apex of Iris Sanguinea Donn ex. Horn. J Nat Sci Res Inst. Vol. 3", pp.93~98 (2005), Catholic University Institute of Natural Sciences
- Lee YM, "Our flower story; Belamcanda chinensis", Kyunghyang Magazine, 93, pp.106~107 (2001)
- Lim JD, "Multiple shoot formation of Gentiana axillariflora leveille by in vitro culture", Kor J Med Crop Sci, 8, pp.41~48 (2000)
- Madubanya LA, "Dierama luteoalbidum: liquid culture provides an efficient system for the ex situ conservation of an endangered and horticulturally valuable plant", S Afr J Bot, 72, pp.584~588 (2006)
- Meyer HJ, "In vitro multiplication of Ixia fkexuosa", HortScience, 23, pp.1070~1071 (1998)
- Murashige T, "A revised medium for rapid growth and bioassays with tobacco tissue culture", Physiol Plant, 15, pp.487~497 (1962)
- Park YJ, "Studies on the seed germination of blackberry lily (Belamcanda chinensis (L.) DC.) native to Korea", J Kor Flower Res Soc, 4, pp.35~40 (1995)
- Raja W, "In vitro microcorm formation in Saffron (Crocus sativus L.)", Acta Hortic, 739, pp.291~296 (2007)
- Ryu JH, "Induction of haploid plants by anther culture in sesame (Sesamum indicum L.)", Kor J Plant Tiss Cult, 19, pp.171~177 (1992)
- Shibli RA, "Somatic embryogenesis in the endermic Black Iris", Plant Cell Tiss Org Cult, 61, pp.15~21 (2000)
- Shim JK, "A taxonomic study on iridaceae in Korea", 박사, pp.1998
- Stefaniak B, "Somatic embryogenesis and plant regeneration of Gladiolus (Gladiolus hort.)", Plant Cell Rep, 13, pp.386~389 (1994)
- Wang Y, "Improved plant regeneration from suspension-cultured cell of Iris germanica L. 'Skating Party'", Hortscience, 34, pp.1271~1276 (1999)
- Yabuya T, "In vitro propagation of Japanese garden iris, Iris ensata Thunb", Euphytica, 57, pp.77~81 (1991)
- 윤인경, "꽃창포 화기조직 절편체 배양으로부터 식물체 분화에 미치는 광ㆍ온도ㆍ당의 영향", Journal of Plant Biotechnology, 30(1), pp.41~45 (2003), 한국식물생명공학회
- Zheng SJ, "Effect of cytokinins and lines on plant regeneration from long-term callus and suspension cultures of Allium cepa L", Euphytica, 108, pp.83~90 (1999)