서양민들레 부위별 추출물의 항산화 및 세포독성 연구
Antioxidant and Cytotoxic Potentials of Methanol Extracts from Taraxacum officinale F. H. Wigg. at Different Plant Parts
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초록
서양민들레의 부위별 성분 및 생리활성 차이를 검토하고자 폴리페놀 함량, 플라보노이드 함량, 항산화성 및 세포독성을 분석하였다. Folin-Denis방법에 따른 총 페놀 함량은 꽃 추출물에서 72.0 mg kg-1으로 가장 높게 나타났으며, 그 다음이 잎, 뿌리, 꽃대 추출물 순으로 나타났다(p＜0.05). 한편, 총 플라보노이드 함량은 총 페놀 함량과 같은경향을 보였으나 더 낮은 범위의 함량이 검출되었다. DPPH 라디컬 소거능은 추출물 농도가 증가할수록 높은 활성을보였으며 그 중 꽃 추출물 (IC50 값 = 624.3 mg kg-1)에서가장 높은 활성을 보였고 그 다음으로 잎, 뿌리, 꽃대 추출물 순으로 나타나(p＜0.05), 이는 페놀 및 플라보노이드함량이 DPPH 라디컬 소거능과 관련이 있음을 보여 주었다.
Abstract
A laboratory experiment was conducted to determine the content of phenolics and flavonoids, antioxidant activity and cytotoxicity from methanol extracts of different plant parts of T. officinale F. H. Wigg. Total phenolics [mg chlorogenic acid equivalents (FAE) kg -1DW] was highest in flower extracts (72.0 mg kg -1), followed by leaf, root, and stalk extracts of T. officinale (p < 0.05). The result of total flavonoid level [mg naringin equivalents kg -1DW] had same tendency to differential total phenolics contents among plant parts, but showed lower ranges of amount. The antioxidant activity of the methanol extracts from all the plant parts dose-dependently increased. DPPH (1,1-diphenyl-2-picryl hydrazyl radical) free radical scavenging activity was highest in flower extracts (IC50 value = 624.3 mg kg -1), and followed by leaf, root, and stalk extracts of T. officinale (p < 0.05). By means of MTT (3-(4,5-dimethylthiazol -2-yl)-2,5-diphenyltetrazolium bromide)assay, cell viability of Calu-6 for human pulmonary carcinoma and SNU-601 for human gastric carcinoma showed the lowest IC50 value in the flower extracts (IC50 value = 85.7 and 311.4 mg kg -1, respectively), indicating the highest cytotoxicity. The results suggested that total phenolics content and total flavonoids level in different plant parts of T. officinale were highly correlated with antioxidative (r 2=0.7280 to 0.9971) or with cytotoxic activities (r 2=0.5795 to 0.9515).
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