The Effects of Korean Cucurbitaceous Plants on the Alkaline Phosphatase Activity Associated with Sonic Hedgehog Pathway
0
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
In order to examine the effects of Korean cucurbitaceous plants on sonic hedgehog pathway and growth of cancer cells with over-activated hedgehog pathway, we measured the sonic hedgehog conditioned medium (shh-CM) induced alkaline phosphatase (ALP) activity and cell viability of pancreatic cancer cell lines by treatment of cucurbitaceous plants. Among the tested cucurbitaceous plants, Actinostemma lobatum Maxim, Cucumis sativus L., Momordica charantia L.,Schizopepon bryoniaefolius Maxim and Trichosanthes kirilowii Max, var. japonica Kitam showed the potent inhibitory effects (> 50 % at 20 μg/mL) on shh-CM induced ALP activity. We also evaluated the cell viability of pancreatic cancer cells treated with the cucurbitaceous plants. The tested cucurbitaceous plants showed the very weak effects on cancer cell proliferation but, T. kirilowii Max, var. japonica Kitam presented the inhibitory effect of 72.7 % on the proliferation of pancreatic cancer cells at 20 μg/mL. Taken together, we screened the effects of Korean cucurbitaceous plants on shh-CM induced ALP activity and cell viability of pancreatic cancers to search for the modulators of the hedgehog pathway leading to the inhibition of cancer cell proliferation. T. kirilowii Max, var. japonica Kitam, among the tested cucurbitaceous plants,showed the inhibitory effects on the shh-CM induced ALP activity and the proliferation of pancreatic cancer cells
Abstract
In order to examine the effects of Korean cucurbitaceous plants on sonic hedgehog pathway and growth of cancer cells with over-activated hedgehog pathway, we measured the sonic hedgehog conditioned medium (shh-CM) induced alkaline phosphatase (ALP) activity and cell viability of pancreatic cancer cell lines by treatment of cucurbitaceous plants. Among the tested cucurbitaceous plants, Actinostemma lobatum Maxim, Cucumis sativus L., Momordica charantia L.,Schizopepon bryoniaefolius Maxim and Trichosanthes kirilowii Max, var. japonica Kitam showed the potent inhibitory effects (> 50 % at 20 μg/mL) on shh-CM induced ALP activity. We also evaluated the cell viability of pancreatic cancer cells treated with the cucurbitaceous plants. The tested cucurbitaceous plants showed the very weak effects on cancer cell proliferation but, T. kirilowii Max, var. japonica Kitam presented the inhibitory effect of 72.7 % on the proliferation of pancreatic cancer cells at 20 μg/mL. Taken together, we screened the effects of Korean cucurbitaceous plants on shh-CM induced ALP activity and cell viability of pancreatic cancers to search for the modulators of the hedgehog pathway leading to the inhibition of cancer cell proliferation. T. kirilowii Max, var. japonica Kitam, among the tested cucurbitaceous plants,showed the inhibitory effects on the shh-CM induced ALP activity and the proliferation of pancreatic cancer cells
참고문헌 (15)
- Cheng, T. C., "Antiproliferation effect and apoptosis mechanism of prostate cancer cell PC-3 by flavonoids and saponins prepared from Gynostemma pentaphyllum", J. Agric. Food Chem, 59, pp.11319~11329 (2011)
- Dakeng, S., "Inhibition of Wnt signaling by cucurbitacin B in breast cancer cells : reduction of Wnt-associated proteins and reduced translocation of galectin-3-mediated β-catenin to the nucleus", J. Cell. Biochem, 113, pp.49~60 (2012)
- Hanahan, D., "Hallmarks of cancer : the next generation", Cell, 144, pp.646~674 (2011)
- Ingham, P. W., "Hedgehog signaling in animal development : paradigms and principles", Genes Dev, 15, pp.3059~3087 (2001)
- 정승민, "천화분이 MCF-7 유방암 세포주의 G2", M 세포주기 억제에 미치는 영향, 2011, pp.25(5), 857~862[DOI]
- Khan, N., "Cancer chemoprevention through dietary antioxidants : progress and promise", Antioxid. Redox Signal, 10, pp.475~510 (2008)
- Lauth, M., "Inhibition of GLI-mediated transcription and tumor cell growth by small-molecule antagonists", Proc. Natl. Acad. Sci USA, 104, pp.8455~8460 (2007)
- Lee, C. C., "Cytotoxicity of plants from Malaysia and Thailand used traditionally to treat cancer", J. Ethnopharmacol, 100, pp.237~243 (2005)
- Li, W., "Cyclic bisdesmosides from Actinostemma lobatum MAXIM(Cucurbitaceae)and their in vitro cytotoxicity", Fitoterapia, 83, pp.147~152 (2012)
- McGovern, P. E., "Anticancer activity of botanical compounds in ancient fermented beverages", Int. J. Oncol, 37, pp.5~14 (2010)
- Nakamura, T., "Induction of osteogenic differentiation by hedgehog proteins", Biochem. Biophys. Res. Commun, 237, pp.465~469 (1997)
- Thayer, S. P., "Hedgehog is an early and late mediator of pancratic cancer tumorigenesis", Nature, 425, pp.851~856 (2003)
- Tian, Y., "Osterix is required for sonic hedgehog-induced osteoblastic MC3T3-E1 cell differentiation", Cell Biochem. Biophys, 64, pp.169~176 (2012)
- Tsuda, N., "Synthetic microRNA designed to target glioma-associated antigen 1 transcription factor inhibits division and induces late apoptosis in pancreatic tumor cells", Clin. Cancer Res, 12, pp.6557~6564 (2006)
- Xu, F. G., "Blockade of hedgehog siganling pathway as a therapeutic strategy for pancreatic cancer", Cancer Lett, 283, pp.119~124 (2009)